shRNA Quality Control
… genes [CDC2, CHUK, KIT, MEKK5, PIM1, STAT1]. HEK293T cells were plated at 2 X 105 per ml for 2ml into a 6-well plate one day before transfection. One ug Hush plasmids were used together with 4 ul TurboFectin 8.0 for transient transfection as suggested by OriGene transfection protocol. Seventy two hrs post transfection cells were lysed. Five ug protein were loaded on Invitrogen…