1.

shRNA Quality Control

… genes [CDC2, CHUK, KIT, MEKK5, PIM1, STAT1]. HEK293T cells were plated at 2 X 105 per ml for 2ml into a 6-well plate one day before transfection. One ug Hush plasmids were used together with 4 ul TurboFectin 8.0 for transient transfection as suggested by OriGene transfection protocol. Seventy two hrs post transfection cells were lysed. Five ug protein were loaded on Invitrogen…

/products/rnai/shrna-plasmids/quality

3.

M1 and M2 Macrophage Markers

… the expression of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α. Additionally, STAT1 and STAT6 transcription factors are also involved in M1 polarization. STAT1 is activated by IFN-γ and promotes the expression of inducible nitric oxide synthase (iNOS), a hallmark of M1 macrophages. M2 macrophage polarization is driven by transcription factors such as STAT6, peroxisome proliferator-activated…

/research-areas/m1-and-m2-macrophage-markers