pEX-C-His Bacterial Expression Vector

CAT#: PS100076

pEX-C-His, bacterial expression vector with C-terminal His tag, 10ug

Product Images

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Specifications

Product Data

• C-Tag: His
• E. coli Selection: Ampicillin (100 ug/mL)
• Features:

  This vector is for E.coli expression.

  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as tagged protein with a C-terminal His tag.
  • Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vector.
  • Detection and purification of the transgene are best done using an anti-His antibody or a nickel affinity column.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal His-tagging vector, PS100030
• Schematic of the multiple cloning sites: