pLenti-N1CR2-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

CAT#: PS100132

pLenti-N1CR2-C-mGFP-P2A-Puro, Lenti vector with N1CR2 promoter, C-terminal mGFP tag and P2A-Puro, 10ug

Product Images

Plasmid Map of pLenti-N1CR2-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector.

Specifications

Product Data

• C-Tag: mGFP
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Features: This Lenti vector is a third generation lentiviral vector.
  
  • The ORF cloned in this vector can be expressed as a tagged protein with C-terminal mGFP tag.
  • The expression of the cloned ORF is driven by N1CR2 promoter, a constitutive promoter.
  • P2APuro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphenicol (34ug/ml)
  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cutandligation process.
  • EF51 Primer can be used to sequence the target sequence after cloning.
  • mGFP_R Primer can be used to sequence the target sequence after cloning.