SARS-CoV-2 Spike S1 Recombinant Protein with His tag

CAT#: TP723917

SARS-CoV-2 Spike S1 Recombinant Protein with His tag

Product Images

Recombinant SARS-COV-2 Spike S1 Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.

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  Immobilized Recombinant SARS-COV-2 Spike S1 at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.5-8.7 ng/mL.

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  The purity of SARS-COV-2 Spike S1 Protein with His tag (Cat.TP723917) was greater than 95% as determined by SEC-HPLC.

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  Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 11.4 nM as determined in a SPR assay (Nicoya OpenSPR).

Specifications

Product Data

• Species: SARS-CoV-2
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  VNLTTRTQLP PAYTNSFTRG VYYPDKVFRS SVLHSTQDLF LPFFSNVTWF HAIHVSGTNG TKRFDNPVLP FNDGVYFAST EKSNIIRGWI FGTTLDSKTQ SLLIVNNATN VVIKVCEFQF CNDPFLGVYY HKNNKSWMES EFRVYSSANN CTFEYVSQPF LMDLEGKQGN FKNLREFVFK NIDGYFKIYS KHTPINLVRD LPQGFSALEP LVDLPIGINI TRFQTLLALH RSYLTPGDSS SGWTAGAAAY YVGYLQPRTF LLKYNENGTI TDAVDCALDP LSETKCTLKS FTVEKGIYQT SNFRVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF ELLHAPATVC GPKKSTNLVK NKCVNFNFNG LTGTGVLTES NKKFLPFQQF GRDIADTTDA VRDPQTLEIL DITPCSFGGV SVITPGTNTS NQVAVLYQDV NCTEVPVAIH ADQLTPTWRV YSTGSNVFQT RAGCLIGAEH VNNSYECDIP IGAGICASYQ TQTNSPRRAR
• Tag: C-6xHis
• Purity: >90% by SDS-PAGE.
• Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Endotoxin: < 1.0 EU/μg of the protein by LAL method.
• Protein Description: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val16-Arg685) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
• Storage: Store at -20°C to -80°C.
• Stability: 3 months

Reference Data

• Locus ID: 43740568