Casein Kinase 2 beta (CSNK2B) (NM_001320) Human Mass Spec Standard

CAT#: PH311299

CSNK2B MS Standard C13 and N15-labeled recombinant protein (NP_001311)

Product Images

Coomassie blue staining of purified CSNK2B protein (Cat# TP311299). The protein was produced from HEK293T cells transfected with CSNK2B cDNA clone (Cat# RC211299) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC211299
• Predicted MW: 24.9 kDa
• Protein Sequence:
  Sequence Link

  >RC211299 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSSEEVSWISWFCGLRGNEFFCEVDEDYIQDKFNLTGLNEQVPHYRQALDMILDLEPDEELEDNPNQSD
  LIEQAAEMLYGLIHARYILTNRGIAQMLEKYQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKC
  MDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLYGFKIHPMAYQLQLQAASNFKSP
  VKTIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001311
• RefSeq Size: 1149
• RefSeq ORF: 645
• Synonyms: CK2B; CK2N; Ckb1; Ckb2; CSK2B; G5A; POBINDS
• Locus ID: 1460
• UniProt ID: P67870, N0E4C7
• Cytogenetics: 6p21.33
• Summary: This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Tight junction, Wnt signaling pathway