ASCL2 (NM_005170) Human Mass Spec Standard

CAT#: PH309250

ASCL2 MS Standard C13 and N15-labeled recombinant protein (NP_005161)

Product Images

Coomassie blue staining of purified ASCL2 protein (Cat# TP309250). The protein was produced from HEK293T cells transfected with ASCL2 cDNA clone (Cat# RC209250) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209250
• Predicted MW: 20.2 kDa
• Protein Sequence:
  Sequence Link

  >RC209250 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDGGTLPRSAPPAPPVPVGCAARRRPASPELLRCSRRRRPATAETGGGAAAVARRNERERNRVKLVNLGF
  QALRQHVPHGGASKKLSKVETLRSAVEYIRALQRLLAEHDAVRNALAGGLRPQAVRPSAPRGPPGTTPVA
  ASPSRASSSPGRGGSSEPGSPRSAYSSDDSGCEGALSPAERELLDFSSWLGGY
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005161
• RefSeq Size: 1864
• RefSeq ORF: 579
• Synonyms: ASH2; bHLHa45; HASH2; MASH2
• Locus ID: 430
• UniProt ID: Q99929
• Cytogenetics: 11p15.5
• Summary: This gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Transcription Factors