TNF alpha (TNF) CytoSection

CAT#: TS406983P5

Transient overexpression of TNF in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Product Images

Immunohistology staining on TNF overexpressed cytosection TS406983P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC206983, 5 micron sections, 40x magnification

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC206983
• Tag: C-MYC/DDK
• Detection Antibodies: DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags (TA592569)
• Target Detection Antibodies: TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: OTI1C1] (TA506513)
• Accession Number: NM_000594, NP_000585
• Other Names: DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
• Storage: Room Temperature
• Stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• Preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• Note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Reference Data

• RefSeq: NP_000585
• Locus ID: 7124
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus