KIF17 Human Gene Knockout Kit (CRISPR)

CAT#: KN209079

KIF17 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: KIF17
• Locus ID: 57576
• Components:

  KN209079G1, KIF17 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GACAACCTTCACCGCCTCGG

  KN209079G2, KIF17 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTCGCTCCCGCTGGTTCAT

  KN209079D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AAGGCAGCGG ACGCGAAAGG AGCCCAGCCA GGGCTGGCGC TTCCCGCAAG CGGCCGCGCG TGGCTGCGGG GATCACTCGG GCGAAGCTCG CGGGCCAGGC GGGGCGTTCC CGGGGCCGCT CTTGTGACGC CACGGGCGTT GCCCAGCAAC CGCGAACGCC GCGGCGGTGA GCTTGCGGGC AGGCGGCTGG GGCGGCTCCG GGCTCGGGTT CTGCGGCGGC GCCTGCAGCG CGGATCCCCC ACGTCCCCGC TCGGGCCGGG CCTCAGAAAC GGGCGAGCCC GGGCGCGGGA GGAAGCGGCG GCCCCGGCTC GGGTCCTGCG ACGCCCAGCT CCCCTCCGGG GCGCCCCCCG AGGGGTCCCC GCCCCCCGCC GCGGCCCCGC CCCTGCCTCA AGGCCCCGCC CCCCGTGGCC GGCGCTGGCC CCGCCCCTTG GCCCGGGCTG CTGGGAGTCC CCGGCGGGGC GGGGGTCAGC TCTGGTCCCG TTGGTCCTGG GCGCGGCGCC GCTGCGCTGC CAGCCCGTGG TGACTGTGGA CTGCGCGCGC GCCCAGTGCT GCATCCAGAA CCCGGGCGCC GCCGACGAGC CGCCCAAGCA GTTCACCTTC GACGGCGCCT ACCACGTGGA CCACGTCACC GAGCAGATCT ACAACGAGAT CGCCTATCCG CTGGTGGAGG TGAGGGCGCC CGGCAGGCCG CCCTGCAGGC GGCAGGGCAT GAGGCCGCCC CGAGAGTCCC CAGGGGGCTG CGCTGCCCCC CAGCCAGGAG GAAAGGCCCT CGAGGGGCGC AGGCGGACGG GAACAGGTAG GTCCGGAGGC TTTTGTCCAG GCGGGACACA ACCAGAGCCT GCCTCTAATG TCGCCTCCGG GTCTGCACTA TCCCCAGCGT CTCCGCCTCC CGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCAAGTAGC TGGGATTACA GGTGCCTGCC ACCACGCCCG TCTAATTTTT GTGTTTTTAG TACAGATGGG GTTTCACCAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001122819, NM_001287212, NM_020816
• UniProt ID: Q9P2E2
• Synonyms: KIF3X; KIF17B; KLP-2; OSM-3
• Summary: Transports vesicles containing N-methyl-D-aspartate (NMDA) receptor 2B along microtubules.[UniProtKB/Swiss-Prot Function]