PTEN Human Gene Knockout Kit (CRISPR)

CAT#: KN202627BN

PTEN - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: PTEN
• Locus ID: 5728
• Components:

  KN202627G1, PTEN gRNA vector 1 in pCas-Guide CRISPR vector

  KN202627G2, PTEN gRNA vector 2 in pCas-Guide CRISPR vector

  KN202627BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  Homologous arm and mBFP-Neo sequences:

  pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet

  AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACTGA CTGACTGACT GCGTCTCAAC CTCTCCTGAA AGGGAAGGTG GAAGCCGTGG GCTCGGGCGG GAGCCGGCTG AGGCGCGGCG GCGGCGGCGG CACCTCCCGC TCCTGGAGCG GGGGGGAGAA GCGGCGGCGG CGGCGGCCGC GGCGGCTGCA GCTCCAGGGA GGGGGTCTGA GTCGCCTGTC ACCATTTCCA GGGCTGGGAA CGCCGGAGAG TTGGTCTCTC CCCTTCTACT GCCTCCAACA CGGCGGCGGC GGCGGCTGGC ACATCCAGGG ACCCGGGCCG GTTTTAAACC TCCCGTGCGC CGCCGCCGCA CCCCCCGTGG CCCGGGCTCC GGAGGCCGCC GGCGGAGGCA GCCGTTCGGA GGATTATTCG TCTTCTCCCC ATTCCGCTGC CGCCGCTGCC AGGCCTCTGG CTGCTGAGGA GAAGCAGGCC CAGTCGCTGC AACCATCCAG CAGCCGCCGC AGCAGCCATT ACCCGGCTGC GGTCCAGAGC CAAGCGGCGG CAGAGCGAGG GGCATCAGCT ACCGCCAAGT CCAGAGCCAT TTCCATCCTG CAGAAGAAGC CCCGCCACCA GCAGCTTCTG CCATCTCTCT CCTCCTTTTT CTTCAGCCAC AGGCTCCCAG ACACTAGCAT GAGCGAGCTG ATTAAGGAGA ACATGCACAT GAAGCTGTAC ATGGAGGGCA CCGTGGACAA CCATCACTTC AAGTGCACAT CCGAGGGCGA AGGCAAGCCC TACGAGGGCA CCCAGACCAT GAGAATCAAG GTGGTCGAGG GCGGCCCTCT CCCCTTCGCC TTCGACATCC TGGCTACTAG CTTCCTCTAC GGCAGCAAGA CCTTCATCAA CCACACCCAG GGCATCCCCG ACTTCTTCAA GCAGTCCTTC CCTGAGGGCT TCACATGGGA GAGAGTCACC ACATACGAAG ACGGGGGCGT GCTGACCGCT ACCCAGGACA CCAGCCTCCA GGACGGCTGC CTCATCTACA ACGTCAAGAT CAGAGGGGTG AACTTCACAT CCAACGGCCC TGTGATGCAG AAGAAAACAC TCGGCTGGGA GGCCTTCACC GAGACGCTGT ACCCCGCTGA CGGCGGCCTG GAAGGCAGAA ACGACATGGC CCTGAAGCTC GTGGGCGGGA GCCATCTGAT CGCAAACATC AAGACCACAT ATAGATCCAA GAAACCCGCT AAGAACCTCA AGATGCCTGG CGTCTACTAT GTGGACTACA GACTGGAAAG AATCAAGGAG GCCAACAACG AGACCTACGT CGAGCAGCAC GAGGTGGCAG TGGCCAGATA CTGCGACCTC CCTAGCAAAC TGGGGCACTA AATCGATCAT ATTCAATAAC CCTTAATATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TTAGGTCTGA AGAGGAGTTT ACGTCCAGCC AAGCTTAGGA TCTCGACCTC GAAATTCTAC CGGGTAGGGG AGGCGCTTTT CCCAAGGCAG TCTGGAGCAT GCGCTTTAGC AGCCCCGCTG GGCACTTGGC GCTACACAAG TGGCCTCTGG CCTCGCACAC ATTCCACATC CACCGGTAGG CGCCAACCGA CTCCGTTCTT TGGTGGCCCC TTCGCGCCAC CTTCTACTCC TCCCCTAGTC AGGAAGTTCC CCCCCGCCCC GCAGCTCGCG TCGTGCAGGA CGTGACAAAT GGAAGTAGCA CGTCTCACTA GTCTCGTGCA GATGGACAGC ACCGCTGAGC AATGGAAGCG GGTAGGCCTT TGGGGCAGCG GCCAATAGCA GCTTTGCTCC TTCGCTTTCT GGGCTCAGAG GCTGGGAAGG GGTGGGTCCG GGGGCGGGCT CAGGGGCGGG CTCAGGGGCG GGGCGGGCGC CCGAAGGTCC TCCGGAGGCC CGGCATTCTG CACGCTTCAA AAGCGCACGT CTGCCGCGCT GTTCTCCTCT TCCTCATCTC CGGGCCTTTC GACCTGCATC CATCTAGATC TCGAGCAGCT GAAGCTTACC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGACGCCC GCCCCACGAC CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGCAT CGATGATATC AGATCCCCGG GATGCAGAAA TTGATGATCT ATTAAACAAT AAAGATGTCC ACTAAAATGG AAGTTTTTCC TGTCATACTT TGTTAAGAAG GGTGAGAACA GAGTACCTAC ATTTTGAATG GAAGGATTGG AGCTACGGGG GTGGGGGTGG GGTGGGATTA GATAAATGCC TGCTCTTTAC TGAAGGCTCT TTACTATTGC TTTATGATAA TGTTTCATAG TTGGATATCA TAATTTAAAC AAGCAAAACC AAATTAAGGG CCAGCTCATT CCTCCCACTC ATGATCTATA GATCTATAGA TCTCTCGTGG GATCATTGTT TTTCTCTTGA TTCCCACTTT GTGGTTCTAA GTACTGTGGT TTCCAAATGT GTCAGTTTCA TAGCCTGAAG AACGAGATCA GCAGCCTCTG TTCCACATAC ACTTCATTCT CAGTATTGTT TTGCCAAGTT CTAATTCCAT CAGAAGCTGG TCGAGATCCG GAACCCTTAA TATAACTTCG TATAATGTAT GCTATACGAA GTTATTAGGT CCCTCGAAGA GGTTCACTAG GCGCGCCAGA CGGATGCGAA AATGTCCGTA GTTTGGGTGA CTATAACATT TAACCCTGGT CAGGTTGCTA GGTCATATAT TTTGTGTTTC CTTTCTGTGT ATTCAACCTA GGGTGTGTTT GGCTAGACGG AACTCTTGCC TGGTTGCAAG TGTCAAGCCA CCGATTGCTT TCTTAGGCTA TCTATATGGT CTCTTCCTGA GGGCTATTGT CCGTTAATAC AGAATACAGT ACACTGTTAG TGGATTAGCG AGCTCGGTAA TCCGGTCTCC TAAATGAACA AAAAAGTAGA CGCTTTTTGA GGTTGAGCAT ATTTCGATTA AATCTTGGCT TAGGCCCTAG ATCAAGGGTT TAGATCAGAA TAAAATGAAA ATTAGTGTTG CACGTACGCA TATTGCATCA GAATCTTGCA GTGATTGTTT TAGTTTCCTG AGTTGCATTG ATAGATTCTT TTAAAATATG ACTGATTTGC ATAACTTTAG AAGCAGAATC ATTTTCAGTA TATATGGTGC ACATTGAGGG CAAAAAGTAG TTTTGTTAAT GTTTAAACTT AAGTTACCTA CAACTTTGAA CTGTATGTAG AAGTTTTGTA GTTTGAAGTC AATAGTGAGC AAGAGACGAC TGACTGACTG ACTGGAAAGA GGAAGGGCTG GAAGAGGAAG GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000314, NM_001304717, NM_001304718
• UniProt ID: P60484
• Synonyms: 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1
• Summary: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]