Grp75 (HSPA9) Human Gene Knockout Kit (CRISPR)

CAT#: KN201397

HSPA9 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Grp75
• Locus ID: 3313
• Components:

  KN201397G1, Grp75 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGTCCCAGTTCCTCACCTGG

  KN201397G2, Grp75 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ATTCTCCAGTATAGGTCAGG

  KN201397D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ACGTCCACGC CCGGGGGGTG TGCACGCGCC CGGGCTCGCC CCAGGGAACG CTGGCGTGCA CTCGGGCACC CAGTGAGCGC CGCAGTTAGT CTCCACCCAT GTTTTCCGCC ACTCCTTGCG CACATGCGCT CTAGGTTCTT AGCTGGATGT CTGTTGCGCC AACAGGCTCC TCCTCCCGCG CAACTGACGC AAGAAGACTG TAATCGGTTC GCAATTTATC CCGTGTGACC TTGAGTCTTT CCTGTCGCCG AGGACCGGGG CTGTCCGAGA TACGGCCTTC ATATCCCTCT TCCTCCCCTG GACTCTTTCT GAGCTCAGAG CCGCCGCAGC CGGGACAGGA GGGCAGGCTT TCTCCAACCA TCATGCTGCG GAGCATATTA CCTGTACGCC CTGGCTCCGG GAGCGGCAGT CGAGTATCCT CTGGTCAGGC GGCGCGGGCG GCGCCTCAGC GGAAGAGCGG GCCTCTGGGC CGCAGTGACC AACCCCCGCC CCTCACCCCA CGTGGTTGGA GGTTTCCAGA AGCGCTGCCG CCACCGCATC GCGCAGCTCT TTGCCGTCGG AGCGCTTGTT TGCTGCCTCG TACTCCTCCA TTTATCCGCC CTTCCATAGT TAGCGCTGCC GGGTCAAGAT TTTGCCCAAG GTTTTGCCTC CGGCTTGGGC CCTGCTGAGT CACACCCCTC GCGATCTAGA GTGTCATTTC AAAATTACCA GGTAGTGGTC CTCATGGCCT GTTCTGTGGC TGAATGGAAA GGTTTGGCCT TGGGGGTGAG GGATGCAGTC TATTGGGAAA GCCACTATTT TCAGTTTTAT TTTTGGCTCC TCTACAGAAT ACAGAGTGTG TAAGGATCCC GTTGATACGG TGAAATAAAA TAAGAAAGCT CTGAGTCGGA ACTCCAGTTT TCGTGCTTAG CAACATTTTC TCAAAATCTG TGGTGTAAGA TTGTACTCGT TTGTTACTTC AGAAAAAAAA GCAGATCGTG GGGCGGAGAA AAGGCTATGG AGTGGTTACT TGTTTTCTAA AATAAAAGAC AAATGCTTTA GAGAACAGGA ATTGTATGTG GGCATTAAGG AAGGCTTGGA AGGAGCCTGA GGTTAAGTTG TAATAAAGGT CTTTTATCCT AGTGAAACGC CACTACTTTT CTTTTTTCTT TCAAAGACCC TTAGTAACTC TAGTTCTTTT AAACTAATTG CACGGCAAGG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_004134
• UniProt ID: P38646
• Synonyms: CSA; GRP-75; GRP75; HEL-S-124m; HSPA9B; MOT; MOT2; MTHSP75; PBP74
• Summary: This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]