DBR1 Human Gene Knockout Kit (CRISPR)

CAT#: KN200024BN

DBR1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: DBR1
• Locus ID: 51163
• Components:

  KN200024G1, DBR1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200024G2, DBR1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200024BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_016216
• UniProt ID: Q9UK59
• Synonyms: debranching enzyme homolog 1; debranching enzyme homolog 1 (S. cerevisiae); RNA lariat debranching enzyme
• Summary: The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011]