Mouse Msh3 activation kit by CRISPRa

CAT#: GA202720

Msh3 CRISPRa kit - CRISPR gene activation of mouse mutS homolog 3

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Msh3
• Locus ID: 17686
• Kit Components:

  GA202720G1, Msh3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA202720G2, Msh3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA202720G3, Msh3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001311120, NM_010829
• UniProt ID: P13705
• Synonyms: D13Em1; Rep-3; Rep3
• Summary: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity).[UniProtKB/Swiss-Prot Function]