Mouse Grin2a activation kit by CRISPRa

CAT#: GA201741

Grin2a CRISPRa kit - CRISPR gene activation of mouse glutamate receptor, ionotropic, NMDA2A (epsilon 1)

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Grin2a
• Locus ID: 14811
• Kit Components:

  GA201741G1, Grin2a gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA201741G2, Grin2a gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA201741G3, Grin2a gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_008170
• UniProt ID: P35436
• Synonyms: GluN2A; NMDAR2A; NR2A
• Summary: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium (PubMed:1374164). Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B (By similarity). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (PubMed:7816096, PubMed:8987814).[UniProtKB/Swiss-Prot Function]