Human SHMT1 activation kit by CRISPRa

CAT#: GA104403

SHMT1 CRISPRa kit - CRISPR gene activation of human serine hydroxymethyltransferase 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: SHMT1
• Locus ID: 6470
• Kit Components:

  GA104403G1, SHMT1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104403G2, SHMT1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104403G3, SHMT1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001281786, NM_004169, NM_148918
• UniProt ID: P34896
• Synonyms: CSHMT; SHMT
• Summary: This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]