Human NRAS activation kit by CRISPRa

CAT#: GA103268

NRAS CRISPRa kit - CRISPR gene activation of human NRAS proto-oncogene, GTPase

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: NRAS
• Locus ID: 4893
• Kit Components:

  GA103268G1, NRAS gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103268G2, NRAS gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103268G3, NRAS gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002524
• UniProt ID: P01111
• Synonyms: ALPS4; CMNS; N-ras; NCMS; NRAS1; NS6
• Summary: This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]