GRK4 (NM_182982) Human Mutant ORF Clone

CAT#: RC403754

GRK4 Mutant (V486A), Myc-DDK-tagged ORF clone of Homo sapiens G protein-coupled receptor kinase 4 (GRK4), transcript variant 1 as transfection-ready DNA

Specifications

Product Data

• Mutation Description: V486A
• Affected Codon#: 486
• Affected NT#: 1457
• Tag: Myc-DDK
• Effect: Essenil hyperension, ssoiion wih
• Symbol: GRK4
• Synonyms: GPRK2L; GPRK4; GRK4a; IT11
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_182982
• ORF Size: 1734 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC403754 representing NM_182982
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGGAGGATATGGCAAAAAAA
  GTGGTCGTAGTAAAAAATGGAAGGAGATACTGACACTGCCTCCTGTCAGCCAGTGCAGTGAGCTTAGACA
  TTCCATTGAAAAGGATTATAGCAGTCTTTGTGACAAGCAACCGATAGGAAGACGTCTCTTCAGGCAGTTC
  TGTGATACCAAACCCACTCTAAAGAGGCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCG
  ATGATGAGGACCGAAGTGATTGTGGACTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCC
  TTTACCAGAAATACCTCCAGATGTTGTGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAA
  AAAGCCTTTGAGGAATGTACTAGAGTTGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAG
  AAAGCTCATATTTTTCTCAGTTTTTACAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATT
  TAGACATTACAGAGTTCTAGGAAAAGGCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGA
  AAAATGTATGCCTGCAAAAAGCTACAAAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAA
  ATGAGAAAAGAATTCTGGAGAAAGTGCAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAA
  AGATGCCTTGTGCTTGGTGCTCACCATTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGC
  AATCCCGGCTTTGATGAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTAC
  AGAGGGAAAGAATTGTATACAGAGACTTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCG
  GATTTCAGACCTCGGTTTGGCCACAGAGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTC
  GGCTACATGGCACCTGAAGTTGTCAATAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCT
  GTCTGATCTATGAAATGATTCAGGGACATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGA
  GGTCGATCAAAGAATCAAGAATGATACCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATC
  TGCAGGATGTTACTCACCAAGAATCCAAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGA
  AGCAGCACCCCGTGTTCAAGGACATCAACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTG
  TCCTGATCCTCATGCCGTTTACTGTAAGGACGTCCTGGATATCGAGCAGTTCTCGGCGGTGAAAGGGATC
  TACCTGGACACCGCAGATGAAGACTTCTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGA
  ATGAGATGATCGAATCTGGGTGTTTCAAAGACATCAACAAAAGTGAAAGTGAGGAAGCTTTGCCATTAGA
  TCTAGACAAGAACATACATACCCCGGTTTCCAGACCAAACAGAGGCTTCTTCTATAGACTCTTCAGAAGA
  GGGGGCTGCCTGACCATGGTCCCCAGTGAGAAGGAAGTGGAACCCAAGCAATGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC403754 representing NM_182982
  Red=Cloning site Green=Tags(s)
  
  MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQF
  CDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSK
  KAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATG
  KMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLG
  NPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTV
  GYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSI
  CRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSAVKGI
  YLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRR
  GGCLTMVPSEKEVEPKQC
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NP_892027
• RefSeq Size: 1734 bp
• RefSeq ORF: 1737 bp
• Locus ID: 2868
• Cytogenetics: 4p16.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 63.6 kDa
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]