GRK1 (NM_002929) Human Mutant ORF Clone

CAT#: RC402682

GRK1 Mutant (R438H), Myc-DDK-tagged ORF clone of Homo sapiens G protein-coupled receptor kinase 1 (GRK1) as transfection-ready DNA

Specifications

Product Data

• Mutation Description: R438H
• Affected Codon#: 438
• Affected NT#: 1313
• Tag: Myc-DDK
• Effect: Reiniis pimenos
• Symbol: GRK1
• Synonyms: GPRK1; RHOK; RK
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_002929
• ORF Size: 1689 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC402682 representing NM_002929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCGGGTCTTTGGAGACCGTGGTGGCCAACTCTGCCTTCATCGCCGCCCGAGGCAGCTTTGACG
  GCAGCAGCTCCCAACCCTCCCGGGACAAGAAGTACCTGGCCAAGCTCAAGCTGCCCCCGCTGTCCAAGTG
  TGAGTCCCTCCGCGACAGCCTCAGCCTGGAGTTTGAGAGTGTGTGCTTGGAGCAGCCCATCGGCAAGAAG
  CTCTTTCAGCAGTTCCTACAATCGGCAGAGAAGCACCTGCCGGCCCTGGAGCTCTGGAAAGACATCGAGG
  ACTATGACACGGCAGACAATGACCTCCAGCCACAGAAGGCCCAGACCATCCTGGCCCAGTACCTGGACCC
  CCAGGCCAAACTCTTCTGCAGCTTCCTGGATGAGGGGATAGTGGCGAAGTTTAAGGAGGGGCCTGTGGAG
  ATCCAGGACGGGCTCTTCCAGCCCCTGCTGCAGGCCACCCTGGCACACCTGGGCCAAGCCCCCTTCCAGG
  AGTACCTGGGCAGCCTGTACTTCCTGAGGTTCCTGCAGTGGAAGTGGCTGGAAGCCCAGCCCATGGGGGA
  GGACTGGTTCCTGGACTTCAGGGTCCTGGGGAAAGGGGGCTTCGGGGAGGTGTCGGCCTGCCAGATGAAG
  GCGACCGGCAAGCTGTATGCCTGCAAGAAGCTGAACAAGAAGCGGCTGAAGAAGAGGAAGGGCTACCAGG
  GTGCTATGGTGGAGAAGAAGATTCTGATGAAAGTACACAGCAGGTTCATCGTGTCTCTGGCCTATGCGTT
  TGAAACCAAAGCCGACCTCTGTCTGGTGATGACCATCATGAACGGAGGTGACATCAGGTACCACATCTAC
  AACGTGAATGAGGAGAACCCTGGCTTCCCGGAGCCGCGCGCCCTCTTCTACACGGCGCAGATCATCTGCG
  GCCTGGAGCACCTGCACCAGAGGCGGATCGTCTACCGCGACCTCAAGCCCGAGAACGTGCTGCTGGACAA
  TGACGGCAATGTCCGGATCTCTGACCTTGGGCTGGCCGTGGAGCTGCTGGACGGACAGAGCAAGACCAAG
  GGCTACGCAGGGACCCCAGGTTTCATGGCCCCCGAGCTCCTGCAGGGCGAGGAGTACGACTTCTCCGTGG
  ACTACTTTGCCCTGGGGGTCACCCTGTATGAGATGATTGCGGCCAGAGGACCCTTCCGAGCCCGTGGAGA
  GAAGGTGGAGAACAAGGAGCTGAAGCACCGGATCATCTCAGAGCCCGTGAAGTACCCTGATAAGTTCAGC
  CAGGCCAGCAAGGACTTCTGCGAGGCGCTGCTGGAGAAGGACCCGGAGAAGCACCTGGGGTTCAGAGATG
  AGACCTGCGACAAGCTCCGTGCCCACCCCCTCTTCAAGGACCTTAACTGGAGGCAGCTGGAGGCTGGGAT
  GCTGATGCCCCCTTTCATCCCAGACTCCAAAACTGTCTACGCAAAGGATATTCAGGACGTGGGTGCCTTT
  TCCACCGTCAAAGGTGTGGCCTTTGACAAAACAGACACAGAATTCTTTCAGGAATTTGCCACTGGCAACT
  GCCCCATCCCCTGGCAGGAGGAGATGATCGAGACGGGCATCTTTGGCGAGCTGAACGTGTGGCGCTCGGA
  CGGTCAGATGCCGGACGACATGAAGGGCATCTCCGGGGGCTCCAGCTCCTCGTCCAAGTCAGGGATGTGT
  CTGGTTTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC402682 representing NM_002929
  Red=Cloning site Green=Tags(s)
  
  MDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKK
  LFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVE
  IQDGLFQPLLQATLAHLGQAPFQEYLGSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGEVSACQMK
  ATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIY
  NVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTK
  GYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFS
  QASKDFCEALLEKDPEKHLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAF
  STVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMC
  LVS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NP_002920
• RefSeq Size: 1689 bp
• RefSeq ORF: 1692 bp
• Locus ID: 6011
• Cytogenetics: 13q34
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 61.9 kDa
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]