RALGDS (NM_001271775) Human Untagged Clone

CAT#: SC333106

RALGDS (untagged) - Homo sapiens ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RALGDS
• Synonyms: RalGEF; RGDS; RGF
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC333106 representing NM_001271775.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGCCCGGGAAGCAGGCCAGGTTTGTGCCAGGCCTGCCGTGCCCAGGGGGAGGAAGGGCTCTGTGTTC
  TTTGCCTGTGTCTCTGTGGTGACCGCCAGGAGAAGGGCCGTCGCCCGTCGTGCCGCTCTCCAAAGCCCG
  ACACCTTGGCTGGCACCTCTGCCAGCACCGGCCACCACCGAGAGCTCCACGCAGGAGATCGGTGAGGAG
  CTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGG
  CAGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTG
  AAGGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTAC
  GTCACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAA
  AGGTACGGTAGATGTGACGCCCTCACGGCCTCCTCTAGATACGGCTGCATCCTCCCCTATTCCGACGAG
  GATGGTGGACCCCAGGACCAACTTAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTAC
  TCGGAGGATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAAC
  ATGCCAGGCTCAGACCTGGAGCGCCGTGCCCACCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATT
  GAGGCAGAGCCTGAGGCTCTGTCACCAGTGCCAGCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTA
  ACACCAGCTCGAGCACCCAGCCCAGTGCCGGCTCCAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCT
  CCAGGTTCAGAGCTAGAAGTAGCTCCAGCACCAGCTCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTG
  GGACTAGAATCGGCTCCAGCGCCAGCTCTGGAACTAGAGCCAGCTCCAGAACAGGATCCAGCTCCCTCA
  CAAACTCTAGAGCTGGAGCCAGCTCCAGCACCAGTTCCATCATTACAGCCTTCCTGGCCTTCACCTGTG
  GTTGCAGAGAACGGGCTGAGTGAGGAGAAGCCTCACCTCTTGGTGTTCCCTCCAGATCTGGTGGCAGAG
  CAGTTTACACTGATGGATGCGGAACTGTTCAAGAAGGTGGTGCCCTACCACTGCCTGGGCTCCATCTGG
  TCCCAGCGGGACAAGAAGGGCAAGGAGCACCTGGCGCCCACCATCCGCGCCACTGTCACCCAGTTCAAC
  AGTGTGGCCAACTGTGTCATCACCACCTGCCTCGGGAACCGAAGCACGAAAGCCCCAGACAGGGCCAGG
  GTGGTGGAGCACTGGATCGAGGTGGCCAGGGAGTGCCGGATCCTCAAGAACTTCTCGTCACTGTATGCC
  ATCCTCTCTGCCCTGCAGAGCAACTCCATCCACCGTCTGAAGAAGACGTGGGAAGACGTTTCCAGGGAC
  AGTTTCCGGATCTTTCAGAAGCTGTCAGAGATCTTCTCAGATGAGAACAACTACTCATTGAGCCGGGAG
  CTGCTCATCAAGGAGGGCACCTCCAAGTTTGCCACCCTGGAGATGAACCCCAAGAGAGCCCAGAAACGG
  CCGAAGGAGACGGGCATCATCCAGGGCACCGTTCCCTACCTGGGCACGTTCCTCACCGACCTGGTGATG
  CTGGACACTGCCATGAAGGACTATCTGTATGGCAGACTCATCAACTTTGAGAAGAGGAGGAAGGAGTTC
  GAGGTGATCGCCCAGATCAAGCTGCTGCAGTCGGCCTGCAACAACTACAGCATCGCGCCAGATGAGCAA
  TTTGGGGCCTGGTTCCGGGCCGTGGAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCGTGCGAGCTG
  GAGCCCCCATCCGAGTCAGCCAGCAACACCCTCAGGACCAAGAAGAACACAGCCATTGTCAAGCGCTGG
  AGCGACCGCCAGGCCCCCAGCACTGAGCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTGTGACCAG
  CTCAGGTGTGGCCCCTACCTCAGCAGCGGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCCGGCTCC
  TCTAGCTCCGACGTGGAGGAGATCAACATCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAAAGAAG
  TTCTGGGAATCAGCCTCACAGTCATCCCCGGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCACCTCG
  TCCTCCTCAGCCTCCACCACGCCCGTGGCTGCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTCTGC
  AACTCCAGCTCCGCGCTGCCGCTCTACAACCAGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCCTG
  GACGTGGACAATGGCAACATGTACAAGAGCATCCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAATC
  CGCAAGGCCATGGACAAACACAACCTGGAGGAGGAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTC
  TCAGATGACCGGAAGCTGAAGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTCTACCGCCAAC
  TATGACTTTGTCCTCAAGAAGCGGACCTTCACCAAGGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACC
  CTCCCTCGCATGAAGCAGAAAGGACTCAAGATTGCCAAGGGCATCTTCTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271775
• Insert Size: 2742 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271775.1
• RefSeq Size: 3714 bp
• RefSeq ORF: 2742 bp
• Locus ID: 5900
• UniProt ID: Q12967
• Cytogenetics: 9q34.13-q34.2
• Protein Pathways: Colorectal cancer, Pancreatic cancer, Pathways in cancer
• MW: 100.3 kDa
• Gene Summary: Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]
  Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region and represents use of an alternate promoter, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1.