RALGDS (NM_001271774) Human Untagged Clone

CAT#: SC333105

RALGDS (untagged) - Homo sapiens ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RALGDS
• Synonyms: RalGEF; RGDS; RGF
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC333105 representing NM_001271774.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTGCCTGTGGGGGCACTCCACAGCCCCTGCCCACACCCTCTCCTCCCCGCCTCTGCTGTTCTGCTCC
  CTGCCCTGTGCCCTCCACTTGCAGCCAGGGACTGGGCATCCACCCGGCCAGGTTCCCAGGAAGAGCTCC
  ACGCAGGAGATCGGTGAGGAGCTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTG
  CACCACGGAGGCAACAAGGGGCAGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAG
  ACTTGCAAGGTGCGGACCGTGAAGGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTC
  CAGGGCAGCGACCTCTCCTACGTCACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAG
  GTCCTGGACCTGCTGTTCAAAAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGGTGGACCCCAG
  GACCAACTTAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAGGATTTCTGT
  CAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAGGCTCAGAC
  CTGGAGCGCCGTGCCCACCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGAGCCTGAG
  GCTCTGTCACCAGTGCCAGCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCTCGAGCA
  CCCAGCCCAGTGCCGGCTCCAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAGAGCTA
  GAAGTAGCTCCAGCACCAGCTCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATCGGCT
  CCAGCGCCAGCTCTGGAACTAGAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAGCTG
  GAGCCAGCTCCAGCACCAGTTCCATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACGGG
  CTGAGTGAGGAGAAGCCTCACCTCTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGATG
  GATGCGGAACTGTTCAAGAAGGTGGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAG
  AAGGGCAAGGAGCACCTGGCGCCCACCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGT
  GTCATCACCACCTGCCTCGGGAACCGAAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGG
  ATCGAGGTGGCCAGGGAGTGCCGGATCCTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTG
  CAGAGCAACTCCATCCACCGTCTGAAGAAGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTT
  CAGAAGCTGTCAGAGATCTTCTCAGATGAGAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAG
  GGCACCTCCAAGTTTGCCACCCTGGAGATGAACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGC
  ATCATCCAGGGCACCGTTCCCTACCTGGGCACGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATG
  AAGGACTATCTGTATGGCAGACTCATCAACTTTGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAG
  ATCAAGCTGCTGCAGTCGGCCTGCAACAACTACAGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTC
  CGGGCCGTGGAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAG
  TCAGCCAGCAACACCCTCAGGACCAAGAAGAACACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCC
  CCCAGCACTGAGCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCC
  TACCTCAGCAGCGGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCCGGCTCCTCTAGCTCCGACGTG
  GAGGAGATCAACATCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAATCAGCC
  TCACAGTCATCCCCGGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCACCTCGTCCTCCTCAGCCTCC
  ACCACGCCCGTGGCTGCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTCTGCAACTCCAGCTCCGCG
  CTGCCGCTCTACAACCAGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCCTGGACGTGGACAATGGC
  AACATGTACAAGAGCATCCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAATCCGCAAGGCCATGGAC
  AAACACAACCTGGAGGAGGAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTCTCAGATGACCGGAAG
  CTGAAGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTCTACCGCCAACTATGACTTTGTCCTC
  AAGAAGCGGACCTTCACCAAGGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACCCTCCCTCGCATGAAG
  CAGAAAGGACTCAAGATTGCCAAGGGCATCTTCTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271774
• Insert Size: 2658 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271774.1
• RefSeq Size: 3621 bp
• RefSeq ORF: 2658 bp
• Locus ID: 5900
• UniProt ID: Q12967
• Cytogenetics: 9q34.13-q34.2
• Protein Pathways: Colorectal cancer, Pancreatic cancer, Pathways in cancer
• MW: 97.3 kDa
• Gene Summary: Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and represents use of an alternate promoter, compared to variant 1. This variant also uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1.