BORIS (CTCFL) (NM_001269042) Human Untagged Clone

CAT#: SC332896

CTCFL (untagged) - Homo sapiens CCCTC-binding factor (zinc finger protein)-like (CTCFL), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BORIS
• Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332896 representing NM_001269042.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGCAGCCACTGAGATCTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAGTTGATGCCG
  GAAAAAGGCCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGACCATCGGAGCCCTAGTGAG
  TTGGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTCCTGGAGGAAGAAGTGGAGCTGGTGCTG
  GCCCCCTCGGAGGAGAGCGAGAAGTACATCCTGACCCTGCAGACGGTGCACTTCACTTCTGAAGCTGTG
  GAGTTGCAGGATATGAGCTTGCTGAGCATACAGCAGCAAGAAGGGGTGCAGGTGGTGGTGCAACAGCCT
  GGCCCTGGGTTGCTGTGGCTTGAGGAAGGGCCCCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATC
  CAGCAAGAGCTGTACTCCCCGCAAGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTGATG
  GTGGCCAGTGAAGACAGTAAGTTAGCGGTGAGCCTGGCTGAAACTACTGGACTGATCAAGCTCGAGGAA
  GAGCAGGAGAAGAACCAGTTATTGGCTGAAAGAACAAAGGAGCAGCTCTTTTTTGTGGAAACAATGTCA
  GGAGATGAAAGAAGTGACGAAATTGTTCTCACAGTTTCAAATTCAAATGTGGAAGAACAAGAGGATCAA
  CCTACAGCTGGTCAAGCAGATGCTGAAAAGGCCAAATCTACAAAAAATCAAAGAAAGACAAAGGGAGCA
  AAAGGAACCTTCCACTGTGATGTCTGCATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATG
  AAAACTCACACCAGTGAGAAGCCTCACCTGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTG
  CTGCGGAACCATGTTAACACCCACACAGGAACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTT
  GTCACCAGTGGAGAACTCGTCCGACACAGGCGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCC
  ATGTGCAAGTATGCCAGTGTGGAGGCAAGTAAATTGAAGCGCCATGTCCGATCCCACACTGGGGAGCGC
  CCCTTTCAGTGTTGCCAGTGCAGCTATGCCAGCAGAGATACCTACAAGCTGAAACGCCACATGAGAACG
  CACTCAGGTGAGAAGCCTTACGAATGCCACATCTGCCACACCCGCTTCACCCAGAGCGGGACCATGAAA
  ATACATATTCTGCAGAAACACGGCGAAAATGTCCCCAAATACCAGTGTCCCCATTGTGCCACCATCATT
  GCACGGAAAAGCGACCTACGTGTGCATATGCGCAACTTGCATGCTTACAGCGCTGCAGAGCTGAAATGC
  CGCTACTGTTCTGCTGTCTTCCATGAACGCTATGCCCTCATTCAGCACCAGAAAACTCATAAGAATGAG
  AAGAGGTTCAAGTGCAAACACTGCAGTTATGCCTGCAAGCAGGAACGTCATATGACCGCTCACATTCGT
  ACCCACACTGGAGAGAAACCATTCACCTGCCTTTCTTGCAATAAATGTTTCCGACAGAAGCAACTTCTA
  AACGCTCACTTCAGGAAATACCACGATGCAAATTTCATCCCGACTGTTTACAAATGCTCCAAGTGTGGC
  AAAGGCTTTTCCCGCTGGATTAACCTGCACAGACATTCGGAGAAGTGTGGATCAGGGGAAGCAAAGTCG
  GCTGCTTCAGGAAAGGGAAGAAGAACAAGAAAGAGGAAGCAGACCATCCTGAAGGAAGCCACAAAGGGT
  CAGAAGGAAGCTGCGAAGGGATGGAAGGAAGCCGCGAACGGAGACGCTGCTGCTGAGGAGGCTTCCACC
  ACGAAGGGAGAACAGTTCCCAGGAGAGATGTTTCCTGTCGCCTGCAGAGAAACCACAGCCAGAGTCAAA
  GAGGAAGTGGATGAAGGCGTGACCTGTGAAATGCTCCTCAACACGATGGATAAGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001269042
• Insert Size: 1989 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001269042.1
• RefSeq Size: 3598 bp
• RefSeq ORF: 1989 bp
• Locus ID: 140690
• UniProt ID: Q8NI51
• Cytogenetics: 20q13.31
• Protein Families: Transcription Factors
• MW: 75.6 kDa
• Gene Summary: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
  Transcript Variant: This variant (4) has an alternate splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal aa in the C-terminal region, compared to isoform 1.