CLOCK (NM_001267843) Human Untagged Clone

CAT#: SC332887

CLOCK (untagged) - Homo sapiens clock circadian regulator (CLOCK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLOCK
• Synonyms: bHLHe8; KAT13D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC332887 representing NM_001267843.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGATGACAGTAGTATTTTTGAT
  GGGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTATCTAGAAACAAATCTGAAAAGAAACGT
  AGAGATCAATTTAATGTTCTCATTAAAGAACTGGGATCCATGCTTCCTGGTAATGCTAGAAAGATGGAC
  AAATCTACTGTTCTGCAGAAAAGCATTGATTTTTTACGAAAACATAAAGAAATCACTGCACAGTCAGAT
  GCTAGTGAAATTCGACAGGACTGGAAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTA
  GAGGCTCTTGATGGTTTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGTGTA
  ACTTCATTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAAGGG
  GAACATTCAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGTGATTCATTAACCCCAGAATAT
  TTAAAATCAAAAAATCAGTTAGAATTCTGTTGTCACATGCTGCGAGGAACAATAGACCCAAAGGAGCCA
  TCTACCTATGAATATGTAAAATTTATAGGAAATTTCAAATCTTTAAACAGTGTATCCTCTTCAGCACAC
  AATGGTTTTGAAGGAACTATACAACGCACACATAGGCCATCTTATGAAGATAGAGTTTGTTTTGTAGCT
  ACTGTCAGGTTAGCTACACCTCAGTTCATCAAGGAAATGTGCACTGTTGAAGAACCCAATGAAGAGTTT
  ACATCTAGACATAGTTTAGAATGGAAGTTTCTGTTTCTAGATCACAGGGCACCACCCATAATAGGGTAT
  TTGCCATTTGAAGTTCTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAATTTGGCA
  AAATGTCATGAGCACTTAATGCAATATGGGAAAGGCAAATCATGTTATTATAGGTTCCTGACTAAGGGG
  CAACAGTGGATTTGGCTTCAGACTCATTATTATATCACTTACCATCAGTGGAATTCAAGGCCAGAGTTT
  ATTGTTTGTACTCACACTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGACGAGAACTTGGCATT
  GAAGAGTCTCTTCCTGAGACAGCTGCTGACAAAAGCCAAGATTCTGGGTCAGATAATCGTATAAACACA
  GTCAGTCTCAAGGAAGCATTGGAAAGGTTTGATCACAGCCCAACCCCTTCTGCCTCTTCTCGGAGTTCA
  AGAAAATCATCTCACACGGCCGTCTCAGACCCTTCCTCAACACCAACCAAGATCCCGACGGATACGAGC
  ACTCCACCCAGGCAGCATTTACCAGCTCATGAGAAGATGGTGCAAAGAAGGTCATCATTTAGTAGTCAG
  TCCATAAATTCCCAGTCTGTTGGTTCATCATTAACACAGCCAGTGATGTCTCAAGCTACAAATTTACCA
  ATTCCACAAGGCATGTCCCAGTTTCAGTTTTCAGCTCAATTAGGAGCCATGCAACATCTGAAAGACCAA
  TTGGAACAACGGACACGCATGATAGAAGCAAATATTCATCGGCAACAAGAAGAACTAAGAAAAATTCAA
  GAACAACTTCAGATGGTCCATGGTCAGGGGCTGCAGATGTTTTTGCAACAATCAAATCCTGGGTTGAAT
  TTTGGTTCCGTTCAACTTTCTTCTGGAAATTCATCTAATATCCAGCAACTTGCACCTATAAATATGCAA
  GGCCAAGTTGTTCCTACTAACCAGATTCAAAGTGGAATGAATACTGGACACATTGGCACAACTCAGCAC
  ATGATACAACAACAGACTTTACAGAGTACATCAACTCAGAGTCAACAAAATGTACTGAGTGGGCACAGT
  CAGCAAACATCTCTACCCAGTCAGACACAGAGCACTCTTACAGCCCCACTGTATAACACTATGGTGATT
  TCTCAGCCTGCAGCCGGAAGCATGGTCCAGATTCCATCTAGTATGCCACAAAACAGCACCCAGAGTGCT
  GCAGTAACTACATTCACTCAGGACAGGCAGATAAGATTTTCTCAAGGTCAACAACTTGTGACCAAATTA
  GTGACTGCTCCTGTAGCTTGTGGGGCAGTCATGGTACCTAGTACTATGCTTATGGGCCAGGTGGTGACT
  GCATATCCTACTTTTGCTACACAACAGCAACAGTCACAGACATTGTCAGTAACGCAGCAGCAGCAGCAG
  CAGAGCTCCCAGGAGCAGCAGCTCACTTCAGTTCAGCAACCATCTCAGGCTCAGCTGACCCAGCCACCG
  CAACAATTTTTACAGACTTCTAGGTTGCTCCATGGGAATCCCTCAACTCAACTCATTCTCTCTGCTGCA
  TTTCCTCTACAACAGAGCACCTTCCCTCAGTCACATCACCAGCAACATCAGTCTCAGCAACAGCAGCAA
  CTCAGCCGGCACAGGACTGACAGCTTGCCCGACCCTTCCAAGGTTCAACCACAGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001267843
• Insert Size: 2541 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267843.1
• RefSeq Size: 10981 bp
• RefSeq ORF: 2541 bp
• Locus ID: 9575
• UniProt ID: O15516
• Cytogenetics: 4q12
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 95.3 kDa
• Gene Summary: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.