NMDAR2A (GRIN2A) (NM_001134408) Human Untagged Clone

CAT#: SC325409

GRIN2A (untagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NMDAR2A
• Synonyms: EPND; FESD; GluN2A; LKS; NMDAR2A; NR2A
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001134408, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAGAGTGGGCTATTGGACCCTGCTGGTGCTGCCGGCCCTTCTGGTCTGGCGCGGTCCGGCGCCGA
  GCGCGGCGGCGGAGAAGGGTCCCCCCGCGCTAAATATTGCGGTGATGCTGGGTCACAGCCACGACGTGAC
  AGAGCGCGAACTTCGAACACTGTGGGGCCCCGAGCAGGCGGCGGGGCTGCCCCTGGACGTGAACGTGGTA
  GCTCTGCTGATGAACCGCACCGACCCCAAGAGCCTCATCACGCACGTGTGCGACCTCATGTCCGGGGCAC
  GCATCCACGGCCTCGTGTTTGGGGACGACACGGACCAGGAGGCCGTAGCCCAGATGCTGGATTTTATCTC
  CTCCCACACCTTCGTCCCCATCTTGGGCATTCATGGGGGCGCATCTATGATCATGGCTGACAAGGATCCG
  ACGTCTACCTTCTTCCAGTTTGGAGCGTCCATCCAGCAGCAAGCCACGGTCATGCTGAAGATCATGCAGG
  ATTATGACTGGCATGTCTTCTCCCTGGTGACCACTATCTTCCCTGGCTACAGGGAATTCATCAGCTTCGT
  CAAGACCACAGTGGACAACAGCTTTGTGGGCTGGGACATGCAGAATGTGATCACACTGGACACTTCCTTT
  GAGGATGCAAAGACACAAGTCCAGCTGAAGAAGATCCACTCTTCTGTCATCTTGCTCTACTGTTCCAAAG
  ACGAGGCTGTTCTCATTCTGAGTGAGGCCCGCTCCCTTGGCCTCACCGGGTATGATTTCTTCTGGATTGT
  CCCCAGCTTGGTCTCTGGGAACACGGAGCTCATCCCAAAAGAGTTTCCATCGGGACTCATTTCTGTCTCC
  TACGATGACTGGGACTACAGCCTGGAGGCGAGAGTGAGGGACGGCATTGGCATCCTAACCACCGCTGCAT
  CTTCTATGCTGGAGAAGTTCTCCTACATCCCCGAGGCCAAGGCCAGCTGCTACGGGCAGATGGAGAGGCC
  AGAGGTCCCGATGCACACCTTGCACCCATTTATGGTCAATGTTACATGGGATGGCAAAGACTTATCCTTC
  ACTGAGGAAGGCTACCAGGTGCACCCCAGGCTGGTGGTGATTGTGCTGAACAAAGACCGGGAATGGGAAA
  AGGTGGGCAAGTGGGAGAACCATACGCTGAGCCTGAGGCACGCCGTGTGGCCCAGGTACAAGTCCTTCTC
  CGACTGTGAGCCGGATGACAACCATCTCAGCATCGTCACCCTGGAGGAGGCCCCATTCGTCATCGTGGAA
  GACATAGACCCCCTGACCGAGACGTGTGTGAGGAACACCGTGCCATGTCGGAAGTTCGTCAAAATCAACA
  ATTCAACCAATGAGGGGATGAATGTGAAGAAATGCTGCAAGGGGTTCTGCATTGATATTCTGAAGAAGCT
  TTCCAGAACTGTGAAGTTTACTTACGACCTCTATCTGGTGACCAATGGGAAGCATGGCAAGAAAGTTAAC
  AATGTGTGGAATGGAATGATCGGTGAAGTGGTCTATCAACGGGCAGTCATGGCAGTTGGCTCGCTCACCA
  TCAATGAGGAACGTTCTGAAGTGGTGGACTTCTCTGTGCCCTTTGTGGAAACGGGAATCAGTGTCATGGT
  TTCAAGAAGTAATGGCACCGTCTCACCTTCTGCTTTTCTAGAACCATTCAGCGCCTCTGTCTGGGTGATG
  ATGTTTGTGATGCTGCTCATTGTTTCTGCCATAGCTGTTTTTGTCTTTGAATACTTCAGCCCTGTTGGAT
  ACAACAGAAACTTAGCCAAAGGGAAAGCACCCCATGGGCCTTCTTTTACAATTGGAAAAGCTATATGGCT
  TCTTTGGGGCCTGGTGTTCAATAACTCCGTGCCTGTCCAGAATCCTAAAGGGACCACCAGCAAGATCATG
  GTATCTGTATGGGCCTTCTTCGCTGTCATATTCCTGGCTAGCTACACAGCCAATCTGGCTGCCTTCATGA
  TCCAAGAGGAATTTGTGGACCAAGTGACCGGCCTCAGTGACAAAAAGTTTCAGAGACCTCATGACTATTC
  CCCACCTTTTCGATTTGGGACAGTGCCTAATGGAAGCACGGAGAGAAACATTCGGAATAACTATCCCTAC
  ATGCATCAGTACATGACCAAATTTAATCAGAAAGGAGTAGAGGACGCCTTGGTCAGCCTGAAAACGGGGA
  AGCTGGACGCTTTCATCTACGATGCCGCAGTCTTGAATTACAAGGCTGGGAGGGATGAAGGCTGCAAGCT
  GGTGACCATCGGGAGTGGGTACATCTTTGCCACCACCGGTTATGGAATTGCCCTTCAGAAAGGCTCTCCT
  TGGAAGAGGCAGATCGACCTGGCCTTGCTTCAGTTTGTGGGTGATGGTGAGATGGAGGAGCTGGAGACCC
  TGTGGCTCACTGGGATCTGCCACAACGAGAAGAACGAGGTGATGAGCAGCCAGCTGGACATTGACAACAT
  GGCGGGCGTATTCTACATGCTGGCTGCCGCCATGGCCCTTAGCCTCATCACCTTCATCTGGGAGCACCTC
  TTCTACTGGAAGCTGCGCTTCTGTTTCACGGGCGTGTGCTCCGACCGGCCTGGGTTGCTCTTCTCCATCA
  GCAGGGGCATCTACAGCTGCATTCATGGAGTGCACATTGAAGAAAAGAAGAAGTCTCCAGACTTCAATCT
  GACGGGATCCCAGAGCAACATGTTAAAACTCCTCCGGTCAGCCAAAAACATTTCCAGCATGTCCAACATG
  AACTCCTCAAGAATGGACTCACCCAAAAGAGCTGCTGACTTCATCCAAAGAGGTTCCCTCATCATGGACA
  TGGTTTCAGATAAGGGGAATTTGATGTACTCAGACAACAGGTCCTTTCAGGGGAAAGAGAGCATTTTTGG
  AGACAACATGAACGAACTCCAAACATTTGTGGCCAACCGGCAGAAGGATAACCTCAATAACTATGTATTC
  CAGGGACAACATCCTCTTACTCTCAATGAGTCCAACCCTAACACGGTGGAGGTGGCCGTGAGCACAGAAT
  CCAAAGCGAACTCTAGACCCCGGCAGCTGTGGAAGAAATCCGTGGATTCCATACGCCAGGATTCACTATC
  CCAGAATCCAGTCTCCCAGAGGGATGAGGCAACAGCAGAGAATAGGACCCACTCCCTAAAGAGCCCTAGG
  TATCTTCCAGAAGAGATGGCCCACTCTGACATTTCAGAAACGTCAAATCGGGCCACGTGCCACAGGGAAC
  CTGACAACAGTAAGAACCACAAAACCAAGGACAACTTTAAAAGGTCAGTGGCCTCCAAATACCCCAAGGA
  CTGTAGTGAGGTCGAGCGCACCTACCTGAAAACCAAATCAAGCTCCCCTAGAGACAAGATCTACACTATA
  GATGGTGAGAAGGAGCCTGGTTTCCACTTAGATCCACCCCAGTTTGTTGAAAATGTGACCCTGCCCGAGA
  ACGTGGACTTCCCGGACCCCTACCAGGATCCCAGTGAAAACTTCCGCAAGGGGGACTCCACGCTGCCAAT
  GAACCGGAACCCCTTGCATAATGAAGAGGGGCTTTCCAACAACGACCAGTATAAACTCTACTCCAAGCAC
  TTCACCTTGAAAGACAAGGGTTCCCCGCACAGTGAGACCAGCGAGCGATACCGGCAGAACTCCACGCACT
  GCAGAAGCTGCCTTTCCAACATGCCCACCTATTCAGGCCACTTCACCATGAGGTCCCCCTTCAAGTGCGA
  TGCCTGCCTGCGGATGGGGAACCTCTATGACATCGATGAAGACCAGATGCTTCAGGAGACAGGGATGACC
  AACGCTTGGTTATTGGGAGATGCCCCTCGGACCCTTACAAACACTCGTTGCCATCCCAGGCGGTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001134408
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134408.1, NP_001127880.1
• RefSeq Size: 4745 bp
• RefSeq ORF: 3846 bp
• Locus ID: 2903
• UniProt ID: Q12879
• Cytogenetics: 16p13.2
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Ion Channels: Sodium, Transmembrane
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Calcium signaling pathway, Long-term potentiation, Neuroactive ligand-receptor interaction, Systemic lupus erythematosus
• Gene Summary: This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (3) has a shorter 5' UTR and is alternatively spliced at the 3' end, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.