GABA A Receptor beta 3 (GABRB3) (NM_000814) Human Untagged Clone

CAT#: SC324637

GABRB3 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABA A Receptor beta 3
• Synonyms: DEE43; ECA5; EIEE43
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000814.4 GCAGCAGCAGGAGCAGCCCCGGCTGCGGGTCGCGACGGCGGCGGGGCGCCCCCTCCCCCG
  TGCCGGGGCGCGGCGGAGGGATGTGGGGCCTTGCGGGAGGAAGGCTTTTCGGCATCTTCT
  CGGCCCCGGTGCTGGTGGCTGTGGTGTGCTGCGCCCAGAGTGTGAACGATCCCGGGAACA
  TGTCCTTTGTGAAGGAGACGGTGGACAAGCTGTTGAAAGGCTACGACATTCGCCTAAGAC
  CCGACTTCGGGGGTCCCCCGGTCTGCGTGGGGATGAACATCGACATCGCCAGCATCGACA
  TGGTTTCCGAAGTCAACATGGATTATACCTTAACCATGTATTTTCAACAATATTGGAGAG
  ATAAAAGGCTCGCCTATTCTGGGATCCCTCTCAACCTCACGCTTGACAATCGAGTGGCTG
  ACCAGCTATGGGTGCCCGACACATATTTCTTAAATGACAAAAAGTCATTTGTGCATGGAG
  TGACAGTGAAAAACCGCATGATCCGTCTTCACCCTGATGGGACAGTGCTGTATGGGCTCA
  GAATCACCACGACAGCAGCATGCATGATGGACCTCAGGAGATACCCCCTGGACGAGCTGA
  ACTGCACTCTGGAAATTGAAAGCTATGGCTACACCACGGATGACATTGAGTTTTACTGGC
  GAGGCGGGGACAAGGCTGTTACCGGAGTGGAAAGGATTGAGCTCCCGCAGTTCTCCATCG
  TGGAGCACCGTCTGGTCTCGAGGAATGTTGTCTTCGCCACAGGTGCCTATCCTCGACTGT
  CACTGAGCTTTCGGTTGAAGAGGAACATTGGATACTTCATTCTTCAGACTTATATGCCCT
  CTATACTGATAACGATTCTGTCGTGGGTGTCCTTCTGGATCAATTATGATGCATCTGCTG
  CTAGAGTTGCCCTCGGGATCACAACTGTGCTGACAATGACAACCATCAACACCCACCTTC
  GGGAGACCTTGCCCAAAATCCCCTATGTCAAAGCCATTGACATGTACCTTATGGGCTGCT
  TCGTCTTTGTGTTCCTGGCCCTTCTGGAGTATGCCTTTGTCAACTACATTTTCTTTGGAA
  GAGGCCCTCAAAGGCAGAAGAAGCTTGCAGAAAAGACAGCCAAGGCAAAGAATGACCGTT
  CAAAGAGCGAAAGCAACCGGGTGGATGCTCATGGAAATATTCTGTTGACATCGCTGGAAG
  TTCACAATGAAATGAATGAGGTCTCAGGCGGCATTGGCGATACCAGGAATTCAGCAATAT
  CCTTTGACAACTCAGGAATCCAGTACAGGAAACAGAGCATGCCTCGAGAAGGGCATGGGC
  GATTCCTGGGGGACAGAAGCCTCCCGCACAAGAAGACCCATCTACGGAGGAGGTCTTCAC
  AGCTCAAAATTAAAATACCTGATCTAACCGATGTGAATGCCATAGACAGATGGTCCAGGA
  TCGTGTTTCCATTCACTTTTTCTCTTTTCAACTTAGTTTACTGGCTGTACTATGTTAACT
  GAGTGACTGTACTTGATTTTTCAAAGACTTCATTTAACACTGAGTGAAATATTACTCTGC
  CTGTCAAGTTTTTATACCTGTACACACACAGACACACAAGCAGACACACACATATATACA
  TACGCAATTGTATATATATGTGAACTTTCTCAGCATATATATAAAATACACGTGTATATG
  AGGATGTATGTGTATATGTTTATACACACAGGAGTCAGTGCCCATGTGTATGGAAGACAA
  ATACACATACATATATACATTTTGCAGCTATGGACAATTTACCACAGGATGCATATTAAA
  GAAAGTCATAGTTTTTTTCTTTTTTAATTGAAAGGGACAAGTATCATCTAAATATTATGC
  CTTGAGAATGAGGGCGTGAAACACAATATCATCCCCAAATGTGTCTTGTATTATCATAAG
  TTAGATGTTTTAGTTTAAAAATCAGAAAGACATTCTTAGTTAATCTTTGAAAACTCATAC
  AGTGGTATTGCTAGTTTAAAATGAGTCACTTACTTCATATCCTCTCGTTCAGTTTAGTAA
  GCAAAGGCTTCTTGGCTTCTCTGGTGATGGGGTTTGTTTTCATCGGGCATACGTTTTCTG
  CAATGGTTTAGTGGCTGGGGTGAGCCACTGGCAGTGTGCTTACCTGTTGTCTGAAACATA
  GATAGATCCCACGTTGATGTCTGAACGACCGTCTTTTGAAAACTCATCGGGAGTGAATGG
  CATCTCGTTGTAAGTACTCTAATATACAGTGTGTAGTTTGTTTCTGTTGTTCACTTGGAG
  TGGATCCAGCTTCACTGTCATGTGCGAACACAGTGACACGTTTGGCCAGTGACATTTCAA
  TCACTGAAAATGTGCTCTACATCTCGTATGGATTTCTAGGCCTGATATCCAACAGAAAGC
  ATAGACGTCTCAGGTTATTCGTTACTCTAAGGTAAAACCATCTAGGATGATTTTCCCCCT
  TGCAGTTATGTTATCATTCTTATAACATTGTATGTTAATAGAAAATATATTTGCATAATA
  TGCATATATATGTATATTTACCAAGATTTTGTTTCTTACGCTTGCTATCATGGCAGCATG
  CGATGTCATATTTTCCTTTATGTGATGTAACTACTTTCTGTTATCTAGAAATTAAGATTG
  AAGCTAAAACACTTCTACTGTTCAATTTCAGAAACTAAGAATCATCCTCATGCCTTTATT
  TCTGTATCTGACATATTTCATAAGCACATCCAACTACTCCTAGACTGACTAGGATTCTGC
  AGGAACATGACCCGTACACACCACGCGTCACCCAACGACCCATGACCGTTCTCTGAGGCA
  AAGGAGGGCAACCTGACAGCAAACACAGTCACTGTTGGTTCCTTCTGATCCACAGCCTCA
  TCAGTATTTGGACTTTTTAAAGCTCGTAGAAACAAGACAAGGTGCACCGGTTTCATAGAC
  GCAACCTTAACTTACTATTTAGATGAGATCTTTCTAAAGAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000814
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000814.4, NP_000805.1
• RefSeq Size: 5811 bp
• RefSeq ORF: 1422 bp
• Locus ID: 2562
• UniProt ID: P28472
• Cytogenetics: 15q12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (1) encodes isoform 1, which is of the same length as isoform 2, but has a distinct N-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.