U1A (SNRPA) (NM_004596) Human Untagged Clone

CAT#: SC320243

SNRPA (untagged)-Human small nuclear ribonucleoprotein polypeptide A (SNRPA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: U1A
• Synonyms: Mud1; U1-A; U1A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004596.3 CTTTGTGGTTTGGTCTCAGGGAAGTAGCAGGCGCCGGTTGAGAGAACTACGGCCCTGTCG
  GAAGGTAACCTCCGGTGCAAACGACCATCGGCGGCAGGCGAGCGGTACGCTTGGCGTCCG
  GGCCTTCCTGGGCCCGTCTGAGGAAACTTGCTGCTCGAGGCCAGGCTGCCTAGGACCTGT
  CCCTTTTTTCTATACTGGCTCCCACATCCGGGTTTTTTCTCCGGGACGGCCCTTCGGATG
  CTTGGGCCAATGGGAATCGCCATTTAGGGTGCTCCGCCCACCGGGTCGCGTAGAGCATCC
  TGGAAGTCGTAGTAAATCTCTCGAGAGTTCTCTCCGCACGCGGGCTGGAGAAGCGGGTCC
  TACGCACGCTTTGTTGTCGCGCTTTGCCTCCGTCCTTCCCCCTACTCCCGCCTTACCTGA
  CTTCCTTTTCGGAGGAAGATCCTTGAGCAGCCGACGTTGGGACAAAGGATTTGGAGAAAC
  CCAGGGCTAAAGTCACGTTTTTCCTCCTTTAAGACTTACCTCAACACTTCACTCCATGGC
  AGTTCCCGAGACCCGCCCTAACCACACTATTTATATCAACAACCTCAATGAGAAGATCAA
  GAAGGATGAGCTAAAAAAGTCCCTGTACGCCATCTTCTCCCAGTTTGGCCAGATCCTGGA
  TATCCTGGTATCACGGAGCCTGAAGATGAGGGGCCAGGCCTTTGTCATCTTCAAGGAGGT
  CAGCAGCGCCACCAACGCCCTGCGCTCCATGCAGGGTTTCCCTTTCTATGACAAACCTAT
  GCGTATCCAGTATGCCAAGACCGACTCAGATATCATTGCCAAGATGAAAGGCACCTTCGT
  GGAGCGGGACCGCAAGCGGGAGAAGAGGAAGCCCAAGAGCCAGGAGACCCCGGCCACCAA
  GAAGGCTGTGCAAGGCGGGGGAGCCACCCCCGTGGTGGGGGCTGTCCAGGGGCCTGTCCC
  GGGCATGCCGCCGATGACTCAGGCGCCCCGCATTATGCACCACATGCCGGGCCAGCCGCC
  CTACATGCCGCCCCCTGGTATGATCCCCCCGCCAGGCCTTGCACCTGGCCAGATCCCACC
  AGGGGCCATGCCCCCGCAGCAGCTTATGCCAGGACAGATGCCCCCTGCCCAGCCTCTTTC
  TGAGAATCCACCGAATCACATCTTGTTCCTCACCAACCTGCCAGAGGAGACCAACGAGCT
  CATGCTGTCCATGCTTTTCAATCAGTTCCCTGGCTTCAAGGAGGTCCGTCTGGTACCCGG
  GCGGCATGACATCGCCTTCGTGGAGTTTGACAATGAGGTACAGGCAGGGGCAGCTCGCGA
  TGCCCTGCAGGGCTTTAAGATCACGCAGAACAACGCCATGAAGATCTCCTTTGCCAAGAA
  GTAGCACCTTTTCCCCCCATGCCTGCCCCTTCCCCTGTTCTGGGGCCACCCCTTTCCCCC
  TTGGCTCAGCCCCCTGAAGGTAAGTCCCCCCTTGGGGGCCTTCTTGGAGCCGTGTGTGAG
  TGAGTGGTCGCCACACAGCATTGTACCCAGAGTCTGTCCCCAGACATTGCACCTGGCGCT
  GTTAGGCCGGAATTAAAGTGGCTTTTTGAGGTTTGGTTTTTCACAAAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004596
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004596.3, NP_004587.1
• RefSeq Size: 1626 bp
• RefSeq ORF: 849 bp
• Locus ID: 6626
• UniProt ID: P09012
• Cytogenetics: 19q13.2
• Domains: RRM
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome
• Gene Summary: The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]