ELOVL1 (NM_022821) Human Untagged Clone

CAT#: SC319359

ELOVL1 (untagged)-Human ELOVL fatty acid elongase 1 (ELOVL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELOVL1
• Synonyms: CGI-88; IKSHD; Ssc1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022821.2 GGCAGGCAGGCTGGCCCCGGGGACTTCTCTCTGGCCCTGCTCCCTCCGAGCGCTCCGCCG
  TTGCCCGCCTGGCCCCTACGGAGTCCTTAGCCAGGATGGAGGCTGTTGTGAACTTGTACC
  AAGAGGTGATGAAGCACGCAGATCCCCGGATCCAGGGCTACCCTCTGATGGGGTCCCCCT
  TGCTAATGACCTCCATTCTCCTGACCTACGTGTACTTCGTTCTCTCACTTGGGCCTCGCA
  TCATGGCTAATCGGAAGCCCTTCCAGCTCCGTGGCTTCATGATTGTCTACAACTTCTCAC
  TGGTGGCACTCTCCCTCTACATTGTCTATGAGTTCCTGATGTCGGGCTGGCTGAGCACCT
  ATACCTGGCGCTGTGACCCTGTGGACTATTCCAACAGCCCTGAGGCACTTAGGATGGTTC
  GGGTGGCCTGGCTCTTCCTCTTCTCCAAGTTCATTGAGCTGATGGACACAGTGATCTTTA
  TTCTCCGAAAGAAAGACGGGCAGGTGACCTTCCTACATGTCTTCCATCACTCTGTGCTTC
  CCTGGAGCTGGTGGTGGGGGGTAAAGATTGCCCCGGGAGGAATGGGCTCTTTCCATGCCA
  TGATAAACTCTTCCGTGCATGTCATAATGTACCTGTACTACGGATTATCTGCCTTTGGCC
  CTGTGGCACAACCCTACCTTTGGTGGAAAAAGCACATGACAGCCATTCAGCTGATCCAGT
  TTGTCCTGGTCTCACTGCACATCTCCCAGTACTACTTTATGTCCAGCTGTAACTACCAGT
  ACCCAGTCATTATTCACCTCATCTGGATGTATGGCACCATCTTCTTCATGCTGTTCTCCA
  ACTTCTGGTATCACTCTTATACCAAGGGCAAGCGGCTGCCCCGTGCACTTCAGCAAAATG
  GAGCTCCAGGTATTGCCAAGGTCAAGGCCAACTGAGAAGCATGGCCTAGATAGGCGCCCA
  CCTAAGTGCCTCAGGACTGCACCTTAGGGCAGTGTCCGTCAGTGCCCTCTCCACCTACAC
  CTGTGACCAAGGCTTATGTGGTCAGGACTGAGCAGGGGACTGGCCCTCCCCTCCCCACAG
  CTGCTCTACAGGGACCACGGCTTTGGTTCCTCACCCACTTCCCCCGGGCAGCTCCAGGGA
  TGTGGCCTCATTGCTGTCTGCCACTCCAGAGCTGGGGGCTAAAAGGGCTGTACAGTTATT
  TCCCCCTCCCTGCCTTAAAACTTGGGAGAGGAGCACTCAGGGCTGGCCCCACAAAGGGTC
  TCGTGGCCTTTTTCCTCACACAGAAGAGGTCAGCAATAATGTCACTGTGGACCCAGTCTC
  ACTCCTCCACCCCACACACTGAAGCAGTAGCTTCTGGGCCAAAGGTCAGGGTGGGCGGGG
  GCCTGGGAATACAGCCTGTGGAGGCTGCTTACTCAACTTGTGTCTTAATTAAAAGTGACA
  GAGGAAACCCCGAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_022821
• Insert Size: 840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022821.2, NP_073732.1
• RefSeq Size: 1501 bp
• RefSeq ORF: 840 bp
• Locus ID: 64834
• UniProt ID: Q9BW60
• Cytogenetics: 1p34.2
• Domains: ELO
• Protein Families: Transmembrane
• Gene Summary: Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that exhibits activity toward saturated and monounsaturated acyl-CoA substrates, with the highest activity towards C22:0 acyl-CoA. May participate in the production of both saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Important for saturated C24:0 and monounsaturated C24:1 sphingolipid synthesis. Indirectly inhibits RPE65 via production of VLCFAs.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein.