THOC2 (NM_001081550) Human Untagged Clone

CAT#: SC315976

THOC2 (untagged)-Human THO complex 2 (THOC2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: THOC2
• Synonyms: CXorf3; dJ506G2.1; hTREX120; MRX12; MRX35; THO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315976 representing NM_001081550.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCCGCGGCTGTGGTGGTTCCCGCAGAGTGGATAAAGAACTGGGAGAAATCAGGGAGAGGCGAA
  TTTTTGCATTTATGTCGGATCCTCAGTGAAAATAAAAGCCATGATAGTTCAACATACAGAGATTTCCAG
  CAAGCTCTCTATGAGTTGTCATATCATGTAATTAAAGGAAATCTAAAGCATGAACAGGCATCTAATGTT
  CTTAGTGACATTAGTGAATTTCGTGAGGATATGCCCTCCATTCTTGCTGATGTATTCTGCATATTAGAC
  ATTGAGACAAATTGTTTAGAAGAAAAAAGCAAGAGAGACTATTTTACACAGTTGGTATTAGCATGTTTG
  TATTTAGTTTCAGACACAGTTCTAAAGGAACGCCTGGATCCAGAAACACTGGAATCATTAGGGCTTATC
  AAACAATCACAGCAATTCAATCAAAAGTCAGTTAAAATCAAGACAAAACTCTTTTATAAGCAGCAAAAA
  TTCAATTTGTTAAGAGAAGAGAATGAAGGTTATGCCAAGCTGATTGCTGAATTGGGGCAAGATTTATCT
  GGAAGTATTACTAGTGATTTAATCTTAGAAAATATCAAATCTTTAATAGGATGCTTTAATCTGGATCCC
  AATAGAGTTTTGGATGTCATTTTAGAAGTGTTTGAATGCAGGCCAGAACACGATGACTTCTTTATATCT
  TTGTTAGAATCTTACATGAGTATGTGTGAACCGCAAACACTGTGTCATATTCTTGGGTTCAAATTCAAG
  TTTTACCAGGAACCAAATGGCGAGACACCATCATCTTTATACAGAGTTGCAGCAGTACTTCTACAATTT
  AATCTTATTGATTTAGATGATCTTTATGTACATCTTCTTCCGGCTGATAATTGCATTATGGATGAACAC
  AAACGAGAAATTGCGGAAGCTAAGCAAATTGTTAGAAAGCTTACGATGGTTGTGTTGTCTTCTGAAAAA
  ATGGATGAGCGAGAGAAAGAAAAGGAAAAAGAAGAGGAGAAAGTAGAGAAACCACCTGATAACCAAAAA
  CTTGGCTTGTTGGAAGCCTTATTAAAGATTGGTGATTGGCAACATGCACAGAACATTATGGATCAGATG
  CCTCCATACTATGCAGCTTCACACAAGCTAATAGCCCTTGCTATTTGCAAGCTCATTCATATAACTATT
  GAGCCTCTCTACCGAAGAGTTGGAGTTCCTAAAGGTGCTAAAGGCTCACCTGTTAATGCTTTGCAAAAC
  AAGAGAGCACCAAAACAAGCTGAGAGCTTTGAAGATTTGAGGAGAGACGTGTTCAATATGTTCTGTTAC
  CTTGGTCCTCACCTTTCTCACGATCCCATTTTATTTGCAAAAGTGGTGCGCATAGGCAAGTCATTTATG
  AAGGAGTTTCAGTCTGATGGAAGCAAACAAGAAGATAAAGAAAAAACGGAAGTTATCCTTAGCTGTTTG
  CTTAGCATTACTGACCAGGTACTACTTCCATCTCTTTCTTTGATGGACTGCAATGCTTGTATGTCTGAG
  GAACTATGGGGAATGTTTAAAACATTTCCATATCAGCATAGATATCGTCTGTATGGCCAGTGGAAGAAT
  GAAACTTATAACAGTCACCCACTTTTAGTAAAAGTTAAAGCTCAAACAATAGACAGAGCCAAATATATC
  ATGAAGCGCCTAACCAAGGAAAATGTGAAGCCTTCTGGAAGACAAATTGGGAAGTTGAGCCACAGCAAT
  CCAACCATTTTGTTTGATTATATCTTGTCACAAATACAGAAGTATGATAACTTAATAACACCTGTAGTA
  GATTCATTGAAATACCTCACTTCACTGAATTATGATGTCTTGGCCTATTGTATCATTGAAGCTTTAGCT
  AATCCAGAAAAGGAAAGAATGAAACATGATGACACAACCATCTCAAGCTGGCTTCAGAGTCTGGCTAGT
  TTCTGTGGTGCAGTTTTTCGTAAATATCCAATTGATCTTGCTGGTCTTCTTCAGTATGTTGCCAATCAG
  CTAAAGGCGGGCAAAAGTTTTGACCTGCTTATATTGAAAGAAGTGGTACAAAAAATGGCAGGAATAGAA
  ATTACAGAGGAAATGACAATGGAGCAACTAGAGGCTATGACTGGTGGAGAGCAGCTAAAAGCTGAGGGT
  GGTTATTTTGGTCAGATCAGAAACACTAAAAAATCCTCTCAGAGATTAAAGGATGCTCTATTGGACCAT
  GATCTTGCCCTTCCTCTCTGTCTGCTTATGGCTCAGCAGAGAAATGGGGTAATCTTTCAGGAAGGTGGA
  GAGAAACATTTGAAACTTGTGGGAAAGCTCTATGACCAGTGTCATGATACCCTGGTGCAGTTTGGTGGG
  TTTTTAGCATCTAATCTGAGCACAGAAGATTATATAAAGCGAGTGCCTTCAATTGATGTACTCTGTAAT
  GAATTTCATACACCCCATGATGCAGCATTTTTCCTGTCTAGGCCAATGTATGCCCATCATATTTCGTCA
  AAGTATGATGAACTTAAAAAATCAGAAAAGGGAAGTAAACAGCAACATAAAGTTCATAAGTACATTACA
  TCATGTGAGATGGTGATGGCGCCTGTCCATGAAGCAGTGGTCTCCTTACATGTTTCCAAAGTCTGGGAT
  GACATCAGCCCTCAATTCTATGCTACATTCTGGTCATTGACAATGTATGACCTTGCAGTTCCACACACC
  AGCTATGAACGAGAAGTCAATAAACTTAAAGTCCAGATGAAAGCAATTGATGACAATCAGGAAATGCCC
  CCAAATAAAAAGAAAAAAGAGAAGGAGCGCTGTACTGCCCTTCAGGACAAGCTTCTTGAAGAAGAAAAG
  AAACAGATGGAACATGTACAGAGAGTTCTACAGAGATTGAAACTGGAAAAGGACAACTGGCTTTTAGCA
  AAATCTACCAAAAATGAGACCATCACAAAATTTCTACAGCTGTGTATATTTCCTCGATGTATTTTTTCA
  GCAATTGATGCTGTTTACTGTGCTCGTTTTGTTGAATTGGTACATCAACAGAAAACTCCAAATTTTTCC
  ACACTTCTTTGCTATGATCGAGTTTTCTCTGACATAATTTACACAGTTGCAAGCTGTACTGAAAATGAA
  GCCAGTCGATACGGAAGGTTTCTTTGCTGCATGTTAGAGACTGTGACCAGGTGGCATAGTGATAGAGCC
  ACATATGAAAAGGAATGTGGAAACTATCCAGGATTCCTTACCATATTACGGGCAACTGGATTTGATGGT
  GGAAATAAGGCTGATCAATTAGACTATGAAAATTTTCGACATGTTGTACATAAATGGCATTACAAACTA
  ACCAAGGCATCGGTACATTGCCTTGAAACAGGCGAATATACTCACATCAGGAATATCTTGATTGTGCTA
  ACAAAAATACTTCCTTGGTACCCAAAAGTTTTGAATCTGGGTCAAGCTTTGGAAAGAAGAGTACACAAA
  ATCTGCCAAGAAGAAAAAGAGAAGAGGCCAGATCTATATGCATTGGCTATGGGCTACTCTGGGCAGTTG
  AAAAGTAGAAAGTCATACATGATACCTGAAAATGAGTTTCATCACAAAGACCCCCCTCCGAGGAATGCA
  GTTGCCAGTGTGCAAAATGGGCCTGGTGGTGGGCCTTCTTCATCATCAATAGGAAGTGCATCTAAATCG
  GATGAAAGCAGTACTGAGGAGACTGATAAATCAAGGGAGAGATCTCAGTGTGGTGTGAAAGCTGTTAAT
  AAAGCTTCTAGTACCACACCTAAAGGGAATTCAAGCAATGGAAATAGTGGCTCTAACAGCAACAAAGCT
  GTTAAAGAAAATGACAAAGAAAAAGGGAAAGAGAAAGAAAAAGAGAAAAAAGAAAAGACTCCAGCTACT
  ACTCCAGAGGCCAGGGTACTTGGTAAAGATGGTAAAGAAAAACCAAAGGAAGAGCGGCCAAATAAAGAT
  GAAAAAGCAAGAGAGACCAAGGAAAGAACGCCGAAGTCTGACAAAGAGAAAGAAAAATTCAAGAAGGAA
  GAAAAAGCTAAAGATGAGAAATTTAAGACCACTGTCCCCAACGCAGAATCAAAATCAACTCAAGAAAGG
  GAAAGAGAGAAGGAGCCATCCAGAGAAAGAGATATAGCAAAGGAAATGAAATCAAAGGAAAATGTTAAA
  GGAGGAGAAAAAACACCAGTTTCTGGGTCCTTGAAATCACCTGTTCCCAGATCAGATATTCCAGAGCCT
  GAAAGGGAACAAAAACGCCGCAAAATTGATACTCACCCTTCTCCATCACATTCCTCCACAGTAAAGGAC
  AGTCTCATCGAACTCAAGGAATCTTCAGCAAAGCTCTACATTAATCATACTCCTCCACCACTGTCCAAG
  AGTAAGGAGAGAGAAATGGACAAGAAAGATTTGGACAAGTCAAGGGAAAGATCCAGAGAAAGAGAGAAA
  AAAGATGAAAAGGACAGGAAAGAGCGGAAAAGGGATCACTCAAACAACGACCGTGAAGTGCCACCGGAC
  TTAACCAAGAGACGTAAAGAGGAGAATGGAACAATGGGGGTTTCAAAACATAAAAGTGAAAGTCCTTGT
  GAATCTCCTTATCCAAATGAGAAAGACAAGGAAAAAAATAAGTCAAAATCTTCAGGCAAAGAAAAAGGC
  AGTGATTCATTTAAATCTGAGAAGATGGATAAAATCTCCTCCGGTGGCAAAAAGGAGTCCAGGCATGAT
  AAAGAAAAGATAGAAAAGAAAGAGAAACGGGACAGTTCAGGAGGAAAGGAAGAGAAGAAACATCATAAG
  TCCTCGGACAAGCACAGATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081550
• Insert Size: 4782 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081550.1
• RefSeq Size: 5625 bp
• RefSeq ORF: 4782 bp
• Locus ID: 57187
• UniProt ID: Q8NI27
• Cytogenetics: Xq25
• Protein Pathways: Spliceosome
• MW: 182.8 kDa
• Gene Summary: The TREX multiprotein complex binds specifically to spliced mRNAs to facilitate mRNA export. The protein encoded by this gene is a member of the THO complex, a subset of the TREX complex. The encoded protein interacts with the THOC1 protein.[provided by RefSeq, Jun 2010]