SCN2A (NM_001040143) Human Untagged Clone

CAT#: SC310931

SCN2A (untagged)-Human sodium channel, voltage-gated, type II, alpha subunit (SCN2A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCN2A
• Synonyms: BFIC3; BFIS3; BFNIS; DEE11; EA9; EIEE11; HBA; HBSCI; HBSCII; Na(v)1.2; NAC2; Nav1.2; SCN2A1; SCN2A2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021007 edited
  AGCAGGATGAAAAGATGGCACAGTCAGTGCTGGTACCGCCAGGACCTGACAGCTTCCGCT
  TCTTTACCAGGGAATCCCTTGCTGCTATTGAACAACGCATTGCAGAAGAGAAAGCTAAGA
  GACCCAAACAGGAACGCAAGGATGAGGATGATGAAAATGGCCCAAAGCCAAACAGTGACT
  TGGAAGCAGGAAAATCTCTTCCATTTATTTATGGAGACATTCCTCCAGAGTATGGTGTCA
  GTGCCCCTGGAGGATCTGGACCCCTACTATATCAATAAGAAAACGTTTATAGTATTGAAT
  AAAGGGAAAGCAATCTCTCGATTCAGTGCCACCCCTGCCCTTTACATTTTAACTCCCTTC
  AACCCTATTAGAAAATTAGCTATTAAGATTTTGGTACATTCTTTATTCAATATGCTCATT
  ATGTGCACGATTCTTACCAACTGTGTATTTATGACCATGAGTAACCCTCCAGACTGGACA
  AAGAATGTGGAGTATACCTTTACAGGAATTTATACTTTTGAATCACTTATTAAAATACTT
  GCAAGGGGCTTTTGTTTAGAAGATTTCACATTTTTACGGGATCCATGGAATTGGTTGGAT
  TTCACAGTCATTACTTTTGCGTATGTAACAGAATTTGTAAACCTAGGCAATGTTTCAGCT
  CTTCGAACTTTCAGAGTCTTGAGAGCTTTGAAAACTATTTCTGTAATTCCAGGCCTGAAG
  ACCATTGTGGGGGCCCTGATCCAGTCAGTGAAGAAGCTTTCTGATGTCATGATCTTGACT
  GTGTTCTGTCTAAGCGTGTTTGCGCTAATAGGATTGCAGTTGTTCATGGGCAACCTACGA
  AATAAATGTTTGCAATGGCCTCCAGATAATTCTTCCTTTGAAATAAATATCACTTCCTTC
  TTTAACAATTCATTGGATGGGAATGGTACTACTTTCAATAGGACAGTGAGCATATTTAAC
  TGGGATGAATATATTGAGGATAAAAGTCACTTTTATTTTTTAGAGGGGCAAAATGATGCT
  CTGCTTTGTGGCAACAGCTCAGATGCAGGCCAGTGTCCTGAAGGATACATCTGTGTGAAG
  GCTGGTAGAAACCCCAACTATGGCTACACGAGCTTTGACACCTTTAGTTGGGCCTTTTTG
  TCCTTATTTCGTCTCATGACTCAAGACTTCTGGGAAAACCTTTATCAACTGACACTACGT
  GCTGCTGGGAAAACGTACATGATATTTTTTGTGCTGGTCATTTTCTTGGGCTCATTCTAT
  CTAATAAATTTGATCTTGGCTGTGGTGGCCATGGCCTATGAGGAACAGAATCAGGCCACA
  TTGGAAGAGGCTGAACAGAAGGAAGCTGAATTTCAGCAGATGCTCGAACAGTTGAAAAAG
  CAACAAGAAGAAGCTCAGGCGGCAGCTGCAGCCGCATCTGCTGAATCAAGAGACTTCAGT
  GGTGCTGGTGGGATAGGAGTTTTTTCAGAGAGTTCTTCAGTAGCATCTAAGTTGAGCTCC
  AAAAGTGAAAAAGAGCTGAAAAACAGAAGAAAGAAAAAGAAACAGAAAGAACAGTCTGGA
  GAAGAAGAGAAAAATGACAGAGTCCGAAAATCGGAATCTGAAGACAGCATAAGAAGAAAA
  GGTTTCCGTTTTTCCTTGGAAGGAAGTAGGCTGACATATGAAAAGAGATTTTCTTCTCCA
  CACCAGTCCTTACTGAGCATCCGTGGCTCCCTTTTCTCTCCAAGACGCAACAGTAGGGCG
  AGCCTTTTCAGCTTCAGAGGTCGAGCAAAGGACATTGGCTCTGAGAATGACTTTGCTGAT
  GATGAGCACAGCACCTTTGAGGACAATGACAGCCGAAGAGACTCTCTGTTCGTGCCGCAC
  AGACATGGAGAACGGCGCCACAGCAATGTCAGCCAGGCCAGCCGTGCCTCCAGGGTGCTC
  CCCATCCTGCCCATGAATGGGAAGATGCATAGCGCTGTGGACTGCAATGGTGTGGTCTCC
  CTGGTCGGGGGCCCTTCTACCCTCACATCTGCTGGGCAGCTCCTACCAGAGGGCACAACT
  ACTGAAACAGAAATAAGAAAGAGACGGTCCAGTTCTTATCATGTTTCCATGGATTTATTG
  GAAGATCCTACATCAAGGCAAAGAGCAATGAGTATAGCCAGTATTTTGACCAACACCATG
  GAAGAACTTGAAGAATCCAGACAGAAATGCCCACCATGCTGGTATAAATTTGCTAATATG
  TGTTTGATTTGGGACTGTTGTAAACCATGGTTAAAGGTGAAACACCTTGTCAACCTGGTT
  GTAATGGACCCATTTGTTGACCTGGCCATCACCATCTGCATTGTCTTAAATACACTCTTC
  ATGGCTATGGAGCACTATCCCATGACGGAGCAGTTCAGCAGTGTACTGTCTGTTGGAAAC
  CTGGTCTTCACAGGGATCTTCACAGCAGAAATGTTTCTCAAGATAATTGCCATGGATCCA
  TATTATTACTTTCAAGAAGGCTGGAATATTTTTGATGGTTTTATTGTGAGCCTTAGTTTA
  ATGGAACTTGGTTTGGCAAATGTGGAAGGATTGTCAGTTCTCCGATCATTCCGGCTGCTC
  CGAGTTTTCAAGTTGGCAAAATCTTGGCCAACTCTAAATATGCTAATTAAGATCATTGGC
  AATTCTGTGGGGGCTCTAGGAAACCTCACCTTGGTATTGGCCATCATCGTCTTCATTTTT
  GCTGTGGTCGGCATGCAGCTCTTTGGTAAGAGCTACAAAGAATGTGTCTGCAAGATTTCC
  AATGATTGTGAACTCCCACGCTGGCACATGCATGACTTTTTCCACTCCTTCCTGATCGTG
  TTCCGCGTGCTGTGTGGAGAGTGGATAGAGACCATGTGGGACTGTATGGAGGTCGCTGGC
  CAAACCATGTGCCTTACTGTCTTCATGATGGTCATGGTGATTGGAAATCTAGTGGTTCTG
  AACCTCTTCTTGGCCTTGCTTTTGAGTTCCTTCAGTTCTGACAATCTTGCTGCCACTGAT
  GATGATAACGAAATGAATAATCTCCAGATTGCTGTGGGAAGGATGCAGAAAGGAATCGAT
  TTTGTTAAAAGAAAAATACGTGAATTTATTCAGAAAGCCTTTGTTAGGAAGCAGAAAGCT
  TTAGATGAAATTAAACCGCTTGAAGATCTAAATAATAAAAAAGACAGCTGTATTTCCAAC
  CATACCACCATAGAAATAGGCAAAGACCTCAATTATCTCAAAGACGGAAATGGAACTACT
  AGTGGCATAGGCAGCAGTGTAGAAAAATATGTCGTGGATGAAAGTGATTACATGTCATTT
  ATAAACAACCCTAGCCTCACTGTGACAGTACCAATTGCTGTTGGAGAATCTGACTTTGAA
  AATTTAAATACTGAAGAATTCAGCAGCGAGTCAGATATGGAGGAAAGCAAAGAGAAGCTA
  AATGCAACTAGTTCATCTGAAGGCAGCACGGTTGATATTGGAGCTCCCGCCGAGGGAGAA
  CAGCCTGAGGTTGAACCTGAGGAATCCCTTGAACCTGAAGCCTGTTTTACAGAAGACTGT
  GTACGGAAGTTCAAGTGTTGTCAGATAAGCATAGAAGAAGGCAAAGGGAAACTCTGGTGG
  AATTTGAGGAAAACATGCTATAAGATAGTGGAGCACAATTGGTTCGAAACCTTCATTGTC
  TTCATGATTCTGCTGAGCAGTGGGGCTCTGGCCTTTGAAGATATATACATTGAGCAGCGA
  AAAACCATTAAGACCATGTTAGAATATGCTGACAAGGTTTTCACTTACATATTCATTCTG
  GAAATGCTGCTAAAGTGGGTTGCATATGGTTTTCAAGTGTATTTTACCAATGCCTGGTGC
  TGGCTAGACTTCCTGATTGTTGATGTCTCACTGGTTAGCTTAACTGCAAATGCCTTGGGT
  TACTCAGAACTTGGTGCCATCAAATCCCTCAGAACACTAAGAGCTCTGAGGCCACTGAGA
  GCTTTGTCCCGGTTTGAAGGAATGAGGGTTGTTGTAAATGCTCTTTTAGGAGCCATTCCA
  TCTATCATGAATGTACTTCTGGTTTGTCTGATCTTTTGGCTAATATTCAGTATCATGGGA
  GTGAATCTCTTTGCTGGCAAGTTTTACCATTGTATTAATTACACCACTGGAGAGATGTTT
  GATGTAAGCGTGGTCAACAACTACAGTGAGTGCAAAGCTCTCATTGAGAGCAATCAAACT
  GCCAGGTGGAAAAATGTGAAAGTAAACTTTGATAACGTAGGACTTGGATATCTGTCTCTA
  CTTCAAGTAGCCACGTTTAAGGGATGGATGGATATTATGTATGCAGCTGTTGATTCACGA
  AATGTAGAATTACAACCCAAGTATGAAGACAACCTGTACATGTATCTTTATTTTGTCATC
  TTTATTATTTTTGGTTCATTCTTTACCTTGAATCTTTTCATTGGTGTCATCATAGATAAC
  TTCAACCAACAGAAAAAGAAGTTTGGAGGTCAAGACATTTTTATGACAGAAGAACAGAAG
  AAATACTACAATGCAATGAAAAAACTGGGTTCAAAGAAACCACAAAAACCCATACCTCGA
  CCTGCTAACAAATTCCAAGGAATGGTCTTTGATTTTGTAACCAAACAAGTCTTTGATATC
  AGCATCATGATCCTCATCTGCCTTAACATGGTCACCATGATGGTGGAAACCGATGACCAG
  AGTCAAGAAATGACAAACATTCTGTACTGGATTAATCTGGTGTTTATTGTTCTGTTCACT
  GGAGAATGTGTGCTGAAACTGATCTCTCTTCGTTACTACTATTTCACTATTGGATGGAAT
  ATTTTTGATTTTGTGGTGGTCATTCTCTCCATTGTAGGAATGTTTCTGGCTGAACTGATA
  GAAAAGTATTTTGTGTCCCCTACCCTGTTCCGAGTGATCCGTCTTGCCAGGATTGGCCGA
  ATCCTACGTCTGATCAAAGGAGCAAAGGGGATCCGCACGCTGCTCTTTGCTTTGATGATG
  TCCCTTCCTGCGTTGTTTAACATCGGCCTCCTTCTTTTCCTGGTCATGTTCATCTACGCC
  ATCTTTGGGATGTCCAATTTTGCCTATGTTAAGAGGGAAGTTGGGATCGATGACATGTTC
  AACTTTGAGACCTTTGGCAACAGCATGATCTGCCTGTTCCAAATTACAACCTCTGCTGGC
  TGGGATGGATTGCTAGCACCTATTCTTAATAGTGGACCTCCAGACTGTGACCCTGACAAA
  GATCACCCTGGAAGCTCAGTTAAAGGAGACTGTGGGAACCCATCTGTTGGGATTTTCTTT
  TTTGTCAGTTACATCATCATATCCTTCCTGGTTGTGGTGAACATGTACATCGCGGTCATC
  CTGGAGAACTTCAGTGTTGCTACTGAAGAAAGTGCAGAGCCTCTGAGTGAGGATGACTTT
  GAGATGTTCTATGAGGTTTGGGAGAAGTTTGATCCCGATGCGACCCAGTTTATAGAGTTT
  GCCAAACTTTCTGATTTTGCAGATGCCCTGGATCCTCCTCTTCTCATAGCAAAACCCAAC
  AAAGTCCAGCTCATTGCCATGGATCTGCCCATGGTGAGTGGTGACCGGATCCACTGTCTT
  GACATCTTATTTGCTTTTACAAAGCGTGTTTTGGGTGAGAGTGGAGAGATGGATGCCCTT
  CGAATACAGATGGAAGAGCGATTCATGGCATCAAACCCCTCCAAAGTCTCTTATGAGCCC
  ATTACGACCACGTTGAAACGCAAACAAGAGGAGGTGTCTGCTATTATTATCCAGAGGGCT
  TACAGACGCTACCTCTTGAAGCAAAAAGTTAAAAAGGTATCAAGTATATACAAGAAAGAC
  AAAGGCAAAGAATGTGATGGAACACCCATCAAAGAAGATACTCTCATTGATAAACTGAAT
  GAGAATTCAACTCCAGAGAAAACCGATATGACGCCTTCCACCACGTCTCCACCCTCGTAT
  GATAGTGTGACCAAACCAGAAAAAGAAAAATTTGAAAAAGACAAATCAGAAAAGGAAGAC
  AAAGGGAAAGATATCAGGGAAAGTAAAAAGTAAAAAGAAACCAAGAATTTTCCATTTTGT
  GATCAATTGTTTACAGCCCGTGATGGTGATGTGTTTGTGTCAACAGGACTCCCACAGGAG
  GTCTATGCCAAACTGACTGTTTTTACAAATGTATACTTAAGGTCAGTGCCTATAACAAGA
  CAGAGACCTCTGGTCAGCAAACTGGAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001040143
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040143.1, NP_001035233.1
• RefSeq Size: 8571 bp
• RefSeq ORF: 6018 bp
• Locus ID: 6326
• UniProt ID: Q99250
• Cytogenetics: 2q24.3
• Protein Families: Druggable Genome, Ion Channels: Sodium, Transmembrane
• Gene Summary: Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with four repeat domains, each of which is composed of six membrane-spanning segments, and one or more regulatory beta subunits. Voltage-gated sodium channels function in the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. Allelic variants of this gene are associated with seizure disorders and autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
  Transcript Variant: This variant (3) contains an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) contains one amino acid substitution relative to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.