VAV2 (NM_003371) Human Untagged Clone

CAT#: SC310084

VAV2 (untagged)-Human vav 2 guanine nucleotide exchange factor (VAV2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAV2
• Synonyms: VAV-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310084 representing NM_003371.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCAGTGGCGGCAGTGCGGCCGCTGGCTCATCGATTGCAAGGTCCTGCCGCCCAACCACCGGGTG
  GTGTGGCCCTCGGCCGTGGTCTTCGACCTGGCGCAGGCGCTGCGCGACGGGGTCCTTCTGTGCCAGCTG
  CTGCACAACCTCTCCCCCGGCTCCATCGACCTCAAGGACATCAACTTCCGGCCGCAGATGTCCCAGTTT
  CTGTGTTTGAAGAACATACGCACCTTCCTGAAAGTCTGCCACGATAAATTTGGATTAAGGAACAGCGAG
  CTGTTTGACCCCTTTGACCTCTTCGATGTGCGAGACTTTGGAAAGGTCATCTCCGCGGTGTCGAGGCTC
  TCCCTGCACAGCATCGCGCAGAACAAAGGGATCAGGCCTTTTCCCTCAGAGGAGACCACAGAGAATGAC
  GATGACGTCTACCGCAGCCTGGAGGAGCTGGCCGACGAGCATGACCTGGGGGAGGACATCTACGACTGC
  GTCCCGTGTGAGGATGGAGGGGACGACATCTACGAGGACATCATCAAGGTGGAGGTGCAGCAGCCCATG
  AAAATGGGCATGACTGAAGATGACAAGAGGAACTGCTGCCTGCTGGAGATCCAGGAGACCGAGGCCAAG
  TACTACCGCACCCTGGAGGACATTGAGAAGAACTACATGAGCCCCCTGCGGCTGGTGCTGAGCCCGGCG
  GACATGGCAGCTGTCTTCATTAACCTGGAGGACCTGATCAAGGTGCATCACAGCTTCCTGAGGGCCATC
  GACGTGTCCGTGATGGTGGGGGGCAGCACGCTGGCCAAGGTCTTCCTCGATTTCAAGGAAAGGCTTCTG
  ATCTACGGGGAGTACTGCAGCCACATGGAGCACGCCCAGAACACACTGAACCAGCTCCTGGCCAGCCGG
  GAGGACTTCAGGCAGAAAGTCGAGGAGTGCACACTGAAGGTCCAGGATGGAAAATTTAAGCTGCAAGAC
  CTGCTGGTGGTCCCCATGCAGAGGGTGCTCAAATACCACCTGCTCTTGAAGGAGCTTCTGAGCCATTCT
  GCGGAACGGCCTGAGAGGCAGCAGCTCAAAGAAGCACTGGAAGCCATGCAGGACTTGGCGATGTACATC
  AATGAAGTTAAACGGGACAAGGAGACCTTGAGGAAAATCAGCGAATTTCAGAGTTCTATAGAAAATTTG
  CAAGTGAAACTGGAGGAATTTGGAAGACCAAAGATTGACGGGGAACTGAAAGTCCGGTCCATAGTCAAC
  CACACCAAGCAGGACAGGTACTTGTTCCTGTTTGACAAGGTGGTCATCGTCTGCAAGCGGAAGGGCTAC
  AGCTACGAGCTCAAGGAGATCATCGAGCTGCTGTTCCACAAGATGACCGACGACCCCATGAACAACAAG
  GACGTCAAGAAGTGGTCCTACGGCTTCTACCTAATTCACCTTCAAGGAAAGCAGGGCTTCCAGTTTTTC
  TGCAAAACAGAAGATATGAAGAGGAAGTGGATGGAGCAGTTTGAGATGGCCATGTCAAACATCAAGCCA
  GACAAAGCCAATGCCAACCACCACAGTTTCCAGATGTACACGTTTGACAAGACCACCAACTGCAAAGCC
  TGCAAAATGTTCCTCAGGGGCACCTTCTACCAGGGATACATGTGTACCAAGTGTGGCGTCGGGGCACAC
  AAGGAGTGCCTGGAAGTGATACCTCCCTGCAAGTTCACTTCTCCTGCAGATCTGGACGCCTCCGGAGCG
  GGACCAGGTCCCAAGATGGTGGCCATGCAGAATTACCATGGCAACCCAGCCCCTCCCGGGAAGCCTGTG
  CTGACCTTCCAGACGGGCGACGTGCTTGAGCTGCTGAGGGGCGACCCTGAGTCTCCGTGGTGGGAGGGT
  CGTCTGGTACAAACCAGGAAGTCAGGGTATTTCCCCAGCTCATCTGTGAAGCCCTGCCCTGTGGATGGA
  AGGCCGCCCATCAGCCGGCCGCCATCCCGGGAGATCGACTACACTGCATACCCCTGGTTTGCAGGTAAC
  ATGGAGAGGCAGCAGACGGACAACCTGCTCAAGTCCCACGCCAGCGGGACCTACCTGATCAGGGAGCGG
  CCTGCCGAGGCTGAGCGCTTTGCAATAAGCATCAAGTTCAATGATGAGGTGAAGCACATCAAGGTGGTG
  GAGAAGGACAACTGGATCCACATCACAGAGGCCAAGAAATTCGACAGCCTCCTGGAGTTGGTGGAGTAC
  TACCAGTGCCACTCACTGAAGGAGAGCTTCAAGCAGCTGGACACCACACTCAAGTACCCCTACAAGTCC
  CGGGAACGTTCGGCCTCCAGGGCCTCCAGCCGGTCCCCAGTGTTCACGCCCCGCGTCATCGGCACAGCT
  GTGGCCAGGTATAACTTTGCCGCCCGAGATATGAGGGAGCTTTCGCTGCGGGAGGGTGACGTGGTGAGG
  ATCTACAGCCGCATCGGCGGAGACCAGGGCTGGTGGAAGGGCGAGACCAACGGACGGATTGGCTGGTTT
  CCTTCAACGTACGTAGAAGAGGAGGGCATCCAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_003371
• Insert Size: 2520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003371.3
• RefSeq Size: 4748 bp
• RefSeq ORF: 2520 bp
• Locus ID: 7410
• UniProt ID: P52735
• Cytogenetics: 9q34.2
• Domains: RhoGEF, SH2, SH3, CH, PH, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway
• MW: 97 kDa
• Gene Summary: VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (2) lacks three single exons in the CDS, as compared to variant 1. The reading frame is not changed and the resulting isoform (2) lacks three internal segments, as compared to isoform 1.