ABCG1 (NM_207627) Human Untagged Clone

CAT#: SC308615

ABCG1 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 1 (ABCG1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCG1
• Synonyms: ABC8; WHITE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308615 representing NM_207627.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGGGAACGCAGGGGTGGACAAAACAGAGAAAGCCCTGCCCTCAGAATGCCAGCAGTTACTCTGCA
  GAGATGACGGAGCCCAAGTCGGTGTGTGTCTCGGTGGATGAGGTGGTGTCCAGCAACATGGAGGCCACT
  GAGACGGACCTGCTGAATGGACATCTGAAAAAAGTAGATAATAACCTCACGGAAGCCCAGCGCTTCTCC
  TCCTTGCCTCGGAGGGCAGCTGTGAACATTGAATTCAGGGACCTTTCCTATTCGGTTCCTGAAGGACCC
  TGGTGGAGGAAGAAAGGATACAAGACCCTCCTGAAAGGAATTTCCGGGAAGTTCAATAGTGGTGAGTTG
  GTGGCCATTATGGGTCCTTCCGGGGCCGGGAAGTCCACGCTGATGAACATCCTGGCTGGATACAGGGAG
  ACGGGCATGAAGGGGGCCGTCCTCATCAACGGCCTGCCCCGGGACCTGCGCTGCTTCCGGAAGGTGTCC
  TGCTACATCATGCAGGATGACATGCTGCTGCCGCATCTCACTGTGCAGGAGGCCATGATGGTGTCGGCA
  CATCTGAAGCTTCAGGAGAAGGATGAAGGCAGAAGGGAAATGGTCAAGGAGATACTGACAGCGCTGGGC
  TTGCTGTCTTGCGCCAACACGCGGACCGGGAGCCTGTCAGGTGGTCAGCGCAAGCGCCTGGCCATCGCG
  CTGGAGCTGGTGAACAACCCTCCAGTCATGTTCTTCGATGAGCCCACCAGCGGCCTGGACAGCGCCTCC
  TGCTTCCAGGTGGTCTCGCTGATGAAAGGGCTCGCTCAAGGGGGTCGCTCCATCATTTGCACCATCCAC
  CAGCCCAGCGCCAAACTCTTCGAGCTGTTCGACCAGCTTTACGTCCTGAGTCAAGGACAATGTGTGTAC
  CGGGGAAAAGTCTGCAATCTTGTGCCATATTTGAGGGATTTGGGTCTGAACTGCCCAACCTACCACAAC
  CCAGCAGATTTTGTCATGGAGGTTGCATCCGGCGAGTACGGTGATCAGAACAGTCGGCTGGTGAGAGCG
  GTTCGGGAGGGCATGTGTGACTCAGACCACAAGAGAGACCTCGGGGGTGATGCCGAGGTGAACCCTTTT
  CTTTGGCACCGGCCCTCTGAAGAGGACTCCTCGTCCATGGAAGGCTGCCACAGCTTCTCTGCCAGCTGC
  CTCACGCAGTTCTGCATCCTCTTCAAGAGGACCTTCCTCAGCATCATGAGGGACTCGGTCCTGACACAC
  CTGCGCATCACCTCGCACATTGGGATCGGCCTCCTCATTGGCCTGCTGTACTTGGGGATCGGGAACGAA
  GCCAAGAAGGTCTTGAGCAACTCCGGCTTCCTCTTCTTCTCCATGCTGTTCCTCATGTTCGCGGCCCTC
  ATGCCTACTGTTCTGACATTTCCCCTGGAGATGGGAGTCTTTCTTCGGGAACACCTGAACTACTGGTAC
  AGCCTGAAGGCCTACTACCTGGCCAAGACCATGGCAGACGTGCCCTTTCAGATCATGTTCCCAGTGGCC
  TACTGCAGCATCGTGTACTGGATGACGTCGCAGCCGTCCGACGCCGTGCGCTTTGTGCTGTTTGCCGCG
  CTGGGCACCATGACCTCCCTGGTGGCACAGTCCCTGGGCCTGCTGATCGGAGCCGCCTCCACGTCCCTG
  CAGGTGGCCACTTTCGTGGGCCCAGTGACAGCCATCCCGGTGCTCCTGTTCTCGGGGTTCTTCGTCAGC
  TTCGACACCATCCCCACGTACCTACAGTGGATGTCCTACATCTCCTATGTCAGGTATGGGTTCGAAGGG
  GTCATCCTCTCCATCTATGGCTTAGACCGGGAAGATCTGCACTGTGACATCGACGAGACGTGCCACTTC
  CAGAAGTCGGAGGCCATCCTGCGGGAGCTGGACGTGGAAAATGCCAAGCTGTACCTGGACTTCATCGTA
  CTCGGGATTTTCTTCATCTCCCTCCGCCTCATTGCCTATTTTGTCCTCAGGTACAAAATCCGGGCAGAG
  AGGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_207627
• Insert Size: 2007 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207627.1
• RefSeq Size: 3142 bp
• RefSeq ORF: 2007 bp
• Locus ID: 9619
• UniProt ID: P45844
• Cytogenetics: 21q22.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 74.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (5) differs in the 5' end-region compared to variant 1, which includes a part of the coding region. The resulting isoform (5) has a distinct and shorter N-terminus, as compared to isoform 1.