IQGAP3 (NM_178229) Human Untagged Clone

CAT#: SC307142

IQGAP3 (untagged)-Human IQ motif containing GTPase activating protein 3 (IQGAP3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IQGAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC307142 representing NM_178229.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGAGGAGAGCAGCGGGCCCAGGCTGGGCAGCCTATGAACGCCTCACAGCTGAGGAGATGGATGAG
  CAGAGGCGGCAGAATGTTGCCTATCAGTACCTGTGCCGGCTGGAGGAGGCCAAGCGCTGGATGGAGGCC
  TGCCTGAAGGAGGAGCTTCCTTCCCCGGTGGAGCTGGAGGAGAGCCTTCGGAATGGAGTGCTGCTGGCC
  AAGCTAGGCCACTGTTTTGCACCCTCCGTGGTTCCCTTGAAGAAGATCTACGATGTGGAGCAGCTGCGG
  TACCAGGCAACTGGCTTACATTTCCGTCACACAGACAACATCAACTTTTGGCTATCTGCAATAGCCCAC
  ATCGGTCTGCCTTCGACCTTCTTCCCAGAGACCACGGACATCTATGACAAAAAGAACATGCCCCGGGTA
  GTCTACTGCATCCATGCTCTCAGTCTCTTCCTCTTCCGGCTGGGATTGGCCCCTCAGATACATGATCTA
  TACGGGAAAGTGAAATTCACAGCTGAGGAACTCAGCAACATGGCGTCCGAACTGGCCAAATATGGCCTC
  CAGCTGCCTGCCTTCAGCAAGATCGGGGGCATCTTGGCCAATGAGCTCTCGGTGGATGAGGCTGCAGTC
  CATGCAGCTGTTCTTGCCATCAATGAAGCAGTGGAGCGAGGGGTGGTGGAGGACACCCTGGCTGCCTTG
  CAGAATCCCAGTGCTCTTCTGGAGAATCTCCGAGAGCCTCTGGCAGCCGTCTACCAAGAGATGCTGGCC
  CAGGCCAAGATGGAGAAGGCAGCCAATGCCAGGAACCATGATGACAGAGAAAGCCAGGACATCTATGAC
  CACTACCTAACTCAGGCTGAAATCCAGGGCAATATCAACCATGTCAACGTCCATGGGGCTCTAGAAGTT
  GTTGATGATGCCCTGGAAAGACAGAGCCCTGAAGCCTTGCTCAAGGCCCTTCAAGACCCTGCCCTGGCC
  CTGCGAGGGGTGAGGAGAGACTTTGCTGACTGGTACCTGGAGCAGCTGAACTCAGACAGAGAGCAGAAG
  GCACAGGAGCTGGGCCTGGTGGAGCTTCTGGAAAAGGAGGAAGTCCAGGCTGGTGTGGCTGCAGCCAAC
  ACAAAGGGTGATCAGGAACAAGCCATGCTCCACGCTGTGCAGCGGATCAACAAAGCCATCCGGAGGGGA
  GTGGCGGCTGACACTGTGAAGGAGCTGATGTGCCCTGAGGCCCAGCTGCCTCCAGTGTACCCTGTTGCA
  TCGTCTATGTACCAGCTGGAGCTGGCAGTGCTCCAGCAGCAGCAGGGGGAGCTTGGCCAGGAGGAGCTC
  TTCGTGGCTGTGGAGATGCTCTCAGCTGTGGTCCTGATTAACCGGGCCCTGGAGGCCCGGGATGCCAGT
  GGCTTCTGGAGCAGCCTGGTGAACCCTGCCACAGGCCTGGCTGAGGTGGAAGGAGAAAATGCCCAGCGT
  TACTTCGATGCCCTGCTGAAATTGCGACAGGAGCGTGGGATGGGTGAGGACTTCCTGAGCTGGAATGAC
  CTGCAGGCCACCGTGAGCCAGGTCAATGCACAGACCCAGGAAGAGACTGACCGGGTCCTTGCAGTCAGC
  CTCATCAATGAGGCTCTGGACAAAGGCAGCCCTGAGAAGACTCTGTCTGCCCTACTGCTTCCTGCAGCT
  GGCCTAGATGATGTCAGCCTCCCTGTCGCCCCTCGGTACCATCTCCTCCTTGTGGCAGCCAAAAGGCAG
  AAGGCCCAGGTGACAGGGGATCCTGGAGCTGTGCTGTGGCTTGAGGAGATCCGCCAGGGAGTGGTCAGA
  GCCAACCAGGACACTAATACAGCTCAGAGAATGGCTCTTGGTGTGGCTGCCATCAATCAAGCCATCAAG
  GAGGGCAAGGCAGCCCAGACTGAGCGGGTGTTGAGGAACCCCGCAGTGGCCCTTCGAGGGGTAGTTCCC
  GACTGTGCCAACGGCTACCAGCGAGCCCTGGAAAGTGCCATGGCAAAGAAACAGCGTCCAGCAGACACA
  GCTTTCTGGGTTCAACATGACATGAAGGATGGCACTGCCTACTACTTCCATCTGCAGACCTTCCAGGGG
  ATCTGGGAGCAACCTCCTGGCTGCCCCCTCAACACCTCTCACCTGACCCGGGAGGAGATCCAGTCAGCT
  GTCACCAAGGTCACTGCTGCCTATGACCGCCAACAGCTCTGGAAAGCCAACGTCGGCTTTGTTATCCAG
  CTCCAGGCCCGCCTCCGTGGCTTCCTAGTTCGGCAGAAGTTTGCTGAGCATTCCCACTTTCTGAGGACC
  TGGCTCCCAGCAGTCATCAAGATCCAGGCTCATTGGCGGGGTTATAGGCAGCGGAAGATTTACCTGGAG
  TGGTTGCAGTATTTTAAAGCAAACCTGGATGCCATAATCAAGATCCAGGCCTGGGCCCGGATGTGGGCA
  GCTCGGAGGCAATACCTGAGGCGTCTGCACTACTTCCAGAAGAATGTTAACTCCATTGTGAAGATCCAG
  GCATTTTTCCGAGCCAGGAAAGCCCAAGATGACTACAGGATATTAGTGCATGCACCCCACCCTCCTCTC
  AGTGTGGTACGCAGATTTGCCCATCTCTTGAATCAAAGCCAGCAAGACTTCTTGGCTGAGGCAGAGCTG
  CTGAAGCTCCAGGAAGAGGTAGTTAGGAAGATCCGATCCAATCAGCAGCTGGAGCAGGACCTCAACATC
  ATGGACATCAAGATTGGCCTGCTGGTGAAGAACCGGATCACTCTGCAGGAAGTGGTCTCCCACTGCAAG
  AAGCTGACCAAGAGGAATAAGGAACAGCTGTCAGATATGATGGTTCTGGACAAGCAGAAGGGTTTAAAG
  TCGCTGAGCAAAGAGAAACGGCAGAAACTAGAAGCATACCAACACCTCTTCTACCTGCTCCAGACTCAG
  CCCATCTACCTGGCCAAGCTGATCTTTCAGATGCCACAGAACAAAACCACCAAGTTCATGGAGGCAGTG
  ATTTTCAGCCTGTACAACTATGCCTCCAGCCGCCGAGAGGCCTATCTCCTGCTCCAGCTGTTCAAGACA
  GCACTCCAGGAGGAAATCAAGTCAAAGGTGGAGCAGCCCCAGGACGTGGTGACAGGCAACCCAACAGTG
  GTGAGGCTGGTGGTGAGATTCTACCGTAATGGGCGGGGACAGAGTGCCCTGCAGGAGATTCTGGGCAAG
  GTTATCCAGGATGTGCTAGAAGACAAAGTGCTCAGCGTCCACACAGACCCTGTCCACCTCTATAAGAAC
  TGGATCAACCAGACTGAGGCCCAGACAGGGCAGCGCAGCCATCTCCCATATGATGTCACCCCGGAGCAG
  GCCTTGAGCCACCCCGAGGTCCAGAGACGACTGGACATCGCCCTACGCAACCTCCTCGCCATGACTGAT
  AAGTTCCTTTTAGCCATCACCTCATCTGTGGACCAAATTCCGTATGGGATGCGATATGTGGCCAAAGTC
  CTGAAGGCAACTCTGGCAGAGAAATTCCCTGACGCCACAGACAGCGAGGTCTATAAGGTGGTCGGGAAC
  CTCCTGTACTACCGCTTCCTGAACCCAGCTGTGGTGGCTCCTGACGCCTTCGACATTGTGGCCATGGCA
  GCTGGTGGAGCCCTGGCTGCCCCCCAGCGCCATGCCCTGGGGGCTGTGGCTCAGCTCCTACAGCACGCT
  GCGGCTGGCAAGGCCTTCTCTGGGCAGAGCCAGCACCTACGGGTCCTGAATGACTATCTGGAGGAAACA
  CACCTCAAGTTCAGGAAGTTCATCCATAGAGCCTGCCAGGTGCCAGAGCCAGAGGAGCGTTTTGCAGTG
  GACGAGTACTCAGACATGGTGGCTGTGGCCAAACCCATGGTGTACATCACCGTGGGGGAGCTGGTCAAC
  ACGCACAGGCTGTTGCTGGAGCACCAGGACTGCATTGCCCCTGATCACCAAGACCCCCTGCATGAGCTC
  CTGGAGGATCTTGGGGAGCTGCCCACCATCCCTGACCTTATTGGTGAGAGCATCGCTGCAGATGGGCAC
  ACGGACCTGAGCAAGCTAGAAGTGTCCCTGACGCTGACCAACAAGTTTGAAGGACTAGAGGCAGATGCT
  GATGACTCCAACACCCGTAGCCTGCTTCTGAGCACCAAGCAGCTGTTGGCCGATATCATACAGTTCCAT
  CCTGGGGACACCCTCAAGGAGATCCTGTCCCTCTCGGCTTCCAGAGAGCAAGAAGCAGCCCACAAGCAG
  CTGATGAGCCGACGCCAGGCCTGTACAGCCCAGACACCGGAGCCACTGCGACGACACCGCTCACTGACA
  GCTCACTCCCTCCTGCCACTGGCAGAGAAGCAGCGGCGCGTCCTGCGGAACCTACGCCGACTTGAAGCC
  CTGGGGTTGGTCAGCGCCAGAAATGGCTACCAGGGGCTAGTGGACGAGCTGGCCAAGGACATCCGCAAC
  CAGCACAGACACAGGCACAGGCGGAAGGCAGAGCTGGTGAAGCTGCAGGCCACATTACAGGGCCTGAGC
  ACTAAGACCACCTTCTATGAGGAGCAGGGTGACTACTACAGCCAGTACATCCGGGCCTGCCTGGACCAC
  CTGGCCCCCGACTCCAAGAGTTCTGGGAAGGGGAAGAAGCAGCCTTCTCTTCATTACACTGCTGCTCAG
  CTCCTGGAAAAGGGTGTCTTGGTGGAAATTGAAGATCTTCCCGCCTCTCACTTCAGAAACGTCATCTTT
  GACATCACGCCGGGAGATGAGGCAGGAAAGTTTGAAGTAAATGCCAAGTTCCTGGGTGTGGACATGGAG
  CGATTTCAGCTTCACTATCAGGATCTCCTGCAGCTCCAGTATGAGGGTGTGGCTGTCATGAAACTCTTC
  AACAAGGCCAAAGTCAATGTCAACCTTCTCATCTTCCTCCTCAACAAGAAGTTTTTGCGGAAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_178229
• Insert Size: 4896 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178229.3
• RefSeq Size: 6069 bp
• RefSeq ORF: 4896 bp
• Locus ID: 128239
• UniProt ID: Q86VI3
• Cytogenetics: 1q22
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 184.6 kDa