RAGE (AGER) (NM_172197) Human Untagged Clone

CAT#: SC306735

AGER (untagged)-Human advanced glycosylation end product-specific receptor (AGER), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAGE
• Synonyms: RAGE; SCARJ1; sRAGE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172197 edited
  GGTCCCTGGAAGGAAGCAGGATGGCAGCCGGAACAGCAGTTGGAGCCTGGGTGCTGGTCC
  TCAGTCTGTGGGGGGCAGTAGTAGGTGCTCAAAACATCACAGCCCGGATTGGCGAGCCAC
  TGGTGCTGAAGTGTAAGGGGGCCCCCAAGAAACCACCCCAGCGGCTGGAATGGAAACTGG
  GAGGAGGCCCCTGGGACAGTGTGGCTCGTGTCCTTCCCAACGGCTCCCTCTTCCTTCCGG
  CTGTCGGGATCCAGGATGAGGGGATTTTCCGGTGCCAGGCAATGAACAGGAATGGAAAGG
  AGACCAAGTCCAACTACCGAGTCCGTGTCTACCAGATTCCTGGGAAGCCAGAAATTGTAG
  ATTCTGCCTCTGAACTCACGGCTGGTGTTCCCAATAAGGTGGGGACATGTGTGTCAGAGG
  GAAGCTACCCTGCAGGGACTCTTAGCTGGCACTTGGATGGGAAGCCCCTGGTGCCTAATG
  AGAAGGGAGTATCTGTGAAGGAACAGACCAGGAGACACCCTGAGACAGGGCTCTTCACAC
  TGCAGTCGGAGCTAATGGTGACCCCAGCCCGGGGAGGAGATCCCCGTCCCACCTTCTCCT
  GTAGCTTCAGCCCAGGCCTTCCCCGACACCGGGCCTTGCGCACAGCCCCCATCCAGCCCC
  GTGTCTGGGAGCCTGTGCCTCTGGAGGAGGTCCAATTGGTGGTGGAGCCAGAAGGTGGAG
  CAGTAGCTCCTGGTGGAACCGTAACCCTGACCTGTGAAGTCCCTGCCCAGCCCTCTCCTC
  AAATCCACTGGATGAAGGATGTGAGTGACCTGGAGAGAGGGGCTGGGAGAACCAGGCGAG
  GAGGGGCCAACTGCAGGCTCTGTGGGAGGATCAGGGCTGGGAACTCTAGCCCTGGCCCTG
  GGGATCCTGGGAGGCCTGGGGACAGCCGCCCTGCTCATTGGGGTCATCTTGTGGCAAAGG
  CGGCAACGCCGAGGAGAGGAGAGGAAGGCCCCAGAAAACCAGGAGGAAGAGGAGGAGCGT
  GCAGAACTGAATCAGTCGGAGGAACCTGAGGCAGGCGAGAGTAGTACTGGAGGGCCTTGA
  GGGGCCCACAGACAGATCCCATCCATCAGCTCCCTTTTCTTTTTCCCTTGAACTGTTCTG
  GCCTCAGACCAACTCTCTCCTGTATAATCTCTCTCCTGTATAACCCCACCTTGCCAAGCT
  TTCTTCTACAACCAGAGCCCCCACAATGATGATTAAACACCTGACACATCTCAAAAAAAA
  AAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172197 unedited
  GGTGCTATATGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCCATTACGGCCGGGGG
  TCCCTGGAAGGAAGCAGGATGGCAGCCGGAACAGCAGTTGGAGCCTGGGTGCTGGTCCTC
  AGTCTGTGGGGGGCAGTAGTAGGTGCTCAAAACATCACAGCCCGGATTGGCGAGCCACTG
  GTGCTGAAGTGTAAGGGGGCCCCCAAGAAACCACCCCAGCGGCTGGAATGGAAACTGGGA
  GGAGGCCCCTGGGACAGTGTGGCTCGTGTCCTTCCCAACGGCTCCCTCTTCCTTCCGGCT
  GTCGGGATCCAGGATGAGGGGATTTTCCGGTGCCAGGCAATGAACAGGAATGGAAAGGAG
  ACCAAGTCCAACTACCGAGTCCGTGTCTACCAGATTCCTGGGAAGCCAGAAATTGTAGAT
  TCTGCCTCTGAACTCACGGCTGGTGTTCCCAATAAGGTGGGGACATGTGTGTCAGAGGGA
  AGCTACCCTGCAGGGACTCTTAGCTGGCACTTGGATGGGAAGCCCCTGGTGCCTAATGAG
  AAGGGAGTATCTGTGAAGGAACAGACCAGGAGACACCCTGAGACAGGGCTCTTCACACTG
  CAGTCGGAGCTAATGGTGACCCCAGCCCGGGGAGGAGATCCCCGTCCCACCTTCTCCTGT
  AGCTTTCAGCCCCAGGCCCTTCCCCGACACCGGGCCTTGCGCACAGCCCCCCATCCAGCC
  CCCGTGTCTGGGAGCCTGTGCCTCTGGAGGAGGTCCAATTGGTGGTGGAGCCAGAAGTGG
  AGCAGTAGCTCCTGGTGGAACCGTAACCCTGACCTGTGAAGTCCCTGCCCAGCCCTCTCC
  TCAATCCACTGATGAAGATGTGAGTGACCTGGAGAGAGGGGCTGGGAGACCAGCGAGAGG
  GCCAACTGCAGCCTCTGTGGGAGGATCAGGGCCTGGGAACCTTCTAGCCCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172197 unedited
  ATGACGTATGCACCCTCAGGGCCGGAAGGCACCTGGGGTAGTGTTGTCACAGGGCACTGC
  CACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGAGGCC
  GAGGCGGCCGACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGATGTGTCAGGTGTTT
  AATCATCATTGTGGGGGCTCTGGTTGTAGAAGAAAGCTTGGCAAGGTGGGGTTATACAGG
  AGAGAGATTATACAGGAGAGAGTTGGTCTGAGGCCAGAACAGTTCAAGGGAAAAAGAAAA
  GGGAGCTGATGGATGGGATCTGTCTGTGGGCCCCTCAAGGCCCTCCAGTACTACTCTCGC
  CTGCCTCAGGTTCCTCCGACTGATTCAGTTCTGCACGCTCCTCCTCTTCCTCCTGGTTTT
  CTGGGGCCTTCCTCTCCTCTCCTCGGCGTTGCCGCCTTTGCCACAAGATGACCCCAATGA
  GCAGGGCGGCTGTCCCCAGGCCTCCCAGGATCCCCAGGGCCAGGGCTAGAGTTCCCAGCC
  CTGATCCTCCCACAGAGCCTGCAGTTGGCCCCTCCTCGCCTGGTTCTCCCAGCCCCTCTC
  TCCAGGTCACTCACATCCTTCATCCAGTGGATTTGAGGAGAGGGCTGGGCAGGGACTTCA
  CAGGTCAGGGTTACGGTTCCACCAGGAGCTACTGCTCCACCTTCTGGCTCCACCACCAAT
  TGGACCTCCTCCAGAGGCACAGGCTCCCAGACACGGGGCTGGATGGGGGCTGTGCGCAAG
  GCCCGGTGTCGGGGAAGGCCTGGGCTGAAGCTACAGGAGAAGTGGGACGGGGATCTCCTC
  CCCGGGCTGGGGTCACCATTAGCTCCGACTGCAGTGTGAAGAGCCTGTCTCAGGTGTCTC
  TGTCTGTCTCACAGATACTCCTCTCATAGGCACCAGGCTTCCATCCAGTGCCAGCTAAAA
  AGTT
  
• Restriction Sites: Please inquire
• ACCN: NM_172197
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172197.1, NP_751947.1
• RefSeq Size: 1259 bp
• RefSeq ORF: 1029 bp
• Locus ID: 177
• UniProt ID: Q15109
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]
  Transcript Variant: This variant (7, also known as RAGE_v16) uses alternate splice sites at several internal coding exons compared to variant 1. This results in a frame-shift and a shorter isoform (7, also known as hRAGEsec) with a distinct C-terminus compared to isoform 1. Sequence Note: This Refseq, containing two in-frame translation initiation codons (at nt 8-10 and nt 101-103), is annotated with a CDS starting from the downstream AUG (dAUG) because the AGE receptor encoded by this gene is a known type 1 transmembrane protein requiring signal peptide for its function, and a signal peptide of 22 aa is predicted for the dAUG initiated protein. Translation initiation from the upstream AUG (uAUG) will add an extra 31 aa to the N-terminus, and no signal peptide is predicted for the uAUG initiated protein.