ERCC1 (NM_202001) Human Untagged Clone

CAT#: SC128251

ERCC1 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERCC1
• Synonyms: COFS4; RAD10; UV20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_202001 edited
  GGGGACCTGACGCTATGGAGCTCTCGGAGTTTTGTGGGGGACGGCTGTGAGTGGGGGGTT
  CCTGCTGCGGGATGAGAACGTAGACGCCAGTGGCTCACTCGCTCCTGGCACCTTCCCTTT
  CAGGCTCCAGATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCC
  AGCAAGGAAGAAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAA
  GCCCTTATTCCGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCC
  TCAGACCTACGCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCC
  CACAGGGTCAGAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAA
  ATCCAACAGCATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCG
  CAACGTGCCCTGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTG
  TGCCCTGTTCCTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCT
  GCAGAGCCTGGGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGA
  TCCCCAGCAGGCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGAT
  CCTCGCCTGGAGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCA
  GAAACCAGCGGACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGGGTGACTGA
  ATGTCTGACCACCGTGAAGTCAGTCAACAAAACGGACAGTCAGACCCTCCTGACCACATT
  TGGATCTCTGGAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCCCAGGCCT
  GGGCCCTCAGAAAGTAAGAGCTCTGGGAAAGAACCCAAGGAGTTGGGGGAAGGAGAGAGC
  CCCAAATAAACACAACCTGAGACCCCAAAGTTTTAAGGTGAAAAAAGAACCAAAGACCAG
  ACACAGTGGCTTCCGCCTGTAATCCCAACATTTTGGGAGGCCAAGGCGGGAGGACTGCTT
  GAGGCCAGAAGTTGGAGACCAGCCTGGGCAAGTGGACACCTCATTTTTACTAAAAAAAAA
  AAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_202001 unedited
  NGGCCGGTTAGATATTTGTATACGACTCATATAGGCGGCCGCGCATTCCCGGGATATCGT
  CGACCCACGCGTCCGTGGGGACCTGACGCTATGGAGCTCTCGGAGTTTTGTGGGGGACGG
  CTGTGAGTGGGGGGTTCCTGCTGCGGGATGAGAACGTAGACGCCAGTGGCTCACTCGCTC
  CNTGGCACCTTCCCTTTCAGGCTCCAGATGGACCCTGGGAAGGACAAAGAGGGGGTGCCC
  CAGCCCTCAGGGCCGCCAGCAAGGAAGAAATTTGTGATACCCCTCGACGAGGATGAGGTC
  CCTCCTGGAGTGGCCAAGCNNCCTTATTCCGATCTACACAGAGCCTTCCCACTGTGGACA
  CCTCGGCCCAGGCGGCCCCTCAGACCTACGCCGAATATGCCATCTCACAGCCTCTGGAAG
  GGGCTGGNGGGCCCACCGTGCCCCACAGGGTCAGAGCCCCTGGCAGGAGAGACGCCCAAC
  CAGGCCCTGAAACCCGGGGCAAAATCCAACAGCATCATTGTGAGCCCTCGGCAGAGGGGC
  AATCCCGTACTGAAGTTCGTGCGCAACGTGCCCTGNGAATTTGGCGACGTAATTCCCGAC
  TATGTGCTGGGCCAGAGCACCTGTGCCCTGTTCCTCAGCCTCCGCTACCACAACCTGCAC
  CCAGACTACATCCATGGGCGGCTGCAGAGCCTGGGGAAGAACTTCGCCTTGNCGGTCCTG
  CTTGTCCCAGTGGATGTGAAAGATCCCCCAGCAGCCCTCAAGGAGCTGGCTAAGATGTGT
  ATCCTGGCCGACTGCACATTGATCCTCGCCTGGAGCCCCGAGAAGCTGGGCGGTACCTGG
  AGACCCTACAGCCTATGAGCAGAAACCAACGGACCTCCTGATGGAGAAGCTAGAGCAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_202001
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_202001.1, NP_973730.1
• RefSeq Size: 1273 bp
• RefSeq ORF: 972 bp
• Locus ID: 2067
• UniProt ID: P07992
• Cytogenetics: 19q13.32
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair
• Gene Summary: The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR and uses a distinct splice pattern in the 3' coding region and 3' UTR, compared to variant 2. The resulting isoform (1) has a longer and distinct C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.