GRK1 (NM_002929) Human Untagged Clone

CAT#: SC128096

GRK1 (untagged)-Human G protein-coupled receptor kinase 1 (GRK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK1
• Synonyms: GPRK1; RHOK; RK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002929 edited
  CTCCTCATTCTTCTCCAAACCTTTATTGAGTACCTACTGTGTGCTGGAATAAGACAGGCA
  GGGCCATGCCCTCATGAAGCTGACAATCCTATTGGTGTGACCATCCCCAGGTGTGTCCCA
  GGTGTGTTGCAGGTGTGTCCGAGGTATGCCCCAGCTGTCCCAGGTGTGCCCCAGCTGTCT
  CAGATGTGCCCCAGCTGTCCCAGGTGTGTCACAGCTGCATTGCAGGTGTGCCCCAGTTGC
  ATTCCATGTGTGCTCCAAGTGTGTACCAGCTGTCCCAGGTGTGTCTCAGGTGTGCCCCAG
  CTGTATCCCAGGTGTGCCTCAGCTGTCTTAGGTGTGTCTCAGGTGCATCCCAGGTGTGTC
  TCAGATGTGCCCCAGCTGTCCCAGGTGTGCCCCAGCTGTCCCAGGTGTGCCCCAGCTGTC
  TCCAGTGTGTCCCAGCTGTGCCCCAGGTGTGTGTCCTAGGTGTGCCTCAGCTGTCTCAGG
  TGTGCCCCAGGCATATCCCAGGTGTGCCCCAGCTGTCCCAGGTGTGTCCTACGTGTGCAC
  CAGCTGTATCCCAGGTGTGCCCCAGGTGTGTCTCAGATGGGTCCCAAGTGTTCCCCAACT
  GCATTTCAGGTGTCTCAGGTGTGCCCAAGCTGTCCCAGGTGTGTCCAAGATGTGCCCCAG
  GTGTGTCTCAGGTGGGTCTCAAGTGCCCCAGCTGCATTTCAGGTGTCTCAGGTGTGCCCC
  CCAGTGCATCCCAGGTGTGTCCCAGGTGTGCCCCAGGTGCATCCCAGGTGTGTCCCAGGT
  GTGCCCCAGCTGTCTCAGGTGTCTCAGGTGTGCCCCAGGCATATCCCAGGTGTGCCTCAG
  CTCTCCCAGGTGTGTCCTACATGTGCACCAGCTGTATCTCAGGTGTGTCTCAGGTGTGCC
  CCAGATGTGCCCCCGGTGTGTCTCAGGTGGGTCCCAAGTGTTCCCCAGCTGCATTTCAAG
  TGTCTCAGGTGTGCCCCAGGTGTGCCCCCGCTGTCCCAGGTGTGTCCAAGATGTACCCCA
  GGTGTGTCCCAGCTGTCCCAAGTGTGTCTCAGGTGTGCCCCAGGTGTGTTCCAGGTGTTC
  CCCAGCTGTCCCAGCTGTCCCAGGTCTCAGGTGTGCCCCAGGTGTGTTCCAGGTGTTCAC
  CAGCTGTCCCAGCTGTCCCAGGTCTCAGGTGTGCCCCAGGTATGTTGCAGGTGTTCCCCA
  GCTGTCCCAGCTGTCCCAGGTGTGTCCCAGGTGTTCCCCAGGTGTGTCCCAGCTGTCCCA
  GGTGTGTCCCAGATGTGCCCCAGGTGTACCCCAGGTGTTTCTCAGGTGGATTCCAGGTGT
  GTCCCAGGTGAGCCCCAGCTGTATTCCATATGCGTCCCTCTGAGTGGGGCCTTGGTTTGA
  TGTAGCTCCGGGGATCTTCTGCTCCCTGGTCCTGGTGTCACCAGCAACTGCCTCTTGACA
  ATCCTGCCTTGCCTGCAAACCCCAGGTGAGAAGAAGACAAATGACTGGGAACTGACCCCT
  CAGTAAGCGCTGGTGGTCTCACCTACAGACCCCCAGGAAGCTGGTCACTGTGGGCTTCTT
  TTCCTCTCTAAATTCCTATTATCAGGTGGTTTTCTTTCTCATTTGCTATTTTCTTAAAAA
  TAAAAATAGGGAAAAACAGCCTTTGTAAATTACGGTTTCTTCCGGCTCCATCCTCTCCGT
  CAGGCCCACATCCCAAGGAAACAGCAGGCTTGAGCCTGGCTGCTGAAGCCAGGGGCTGGA
  TGGAGCAGCTCAGAACAGAGCTTTGAGTGCCTCTCCAGCCAGGGGCCCCAGAAGCCTGGT
  GGTTGTTTGTCCTTCTCAGGGGAAAAGTGAGGCGGCCCCTTGGAGGAAGGGGCCGGGCAG
  AATGATCTAATCGGATTCCAAGCAGCTCAGGGGATTGTCTTTTTCTAGCACCTTCTTGCC
  ACTCCTAAGCGTCCTCCGTGACCCCGGCTGGGATTTAGCCTGGTGCTGTGTCAGCCCCGG
  GCTCCCAGGGGCTTCCCAGTGGTCCCCAGGAACCCTCGACAGGGCCAGGGCGTCTCTCTC
  GTCCAGCAAGGGCAGGGACGGGCCACAGGCCAAGGGCAGCAGTCAGGCCTGCTCTGTCTG
  TGAACGCTCCCGGCTTGGCCTCGGCTGATGGGCCCTCACGCCTGAAGCGGGCAGGAAGCT
  CCGGGATGGATTTCGGGTCTTTGGAGACCGTGGTGGCCAACTCTGCCTTCATCGCCGCCC
  GAGGCAGCTTTGACGGCAGCAGCTCCCAACCCTCCCGGGACAAGAAGTACCTGGCCAAGC
  TCAAGCTGCCCCCGCTGTCCAAGTGTGAGTCCCTCCGCGACAGCCTCAGCCTGGAGTTTG
  AGAGTGTGTGCTTGGAGCAGCCCATCGGCAAGAAGCTCTTTCAGCAGTTCCTACAATCGG
  CAGAGAAGCACCTGCCGGCCCTGGAGCTCTGGAAAGACATCGAGGACTATGACACGGCAG
  ACAATGACCTCCAGCCACAGAAGGCCCAGACCATCCTGGCCCAGTACCTGGACCCCCAGG
  CCAAACTCTTCTGCAGCTTCCTGGATGAGGGGATAGTGGCGAAGTTTAAGGAGGGGCCTG
  TGGAGATCCAGGACGGGCTCTTCCAGCCCCTGCTGCAGGCCACCCTGGCACACCTGGGCC
  AAGCCCCCTTCCAGGAGTACCTGGGCAGCCTGTACTTCCTGAGGTTCCTGCAGTGGAAGT
  GGCTGGAAGCCCAGCCCATGGGGGAGGACTGGTTCCTGGACTTCAGGGTCCTGGGGAAAG
  GGGGCTTCGGGGAGGTGTCGGCCTGCCAGATGAAGGCGACCGGCAAGCTGTATGCCTGCA
  AGAAGCTGAACAAGAAGCGGCTGAAGAAGAGGAAGGGCTACCAGGGTGCTATGGTGGAGA
  AGAAGATTCTGATGAAAGTACACAGCAGGTTCATCGTGTCTCTGGCCTATGCGTTTGAAA
  CCAAAGCCGACCTCTGTCTGGTGATGACCATCATGAACGGAGGTGACATCAGGTACCACA
  TCTACAACGTGAATGAGGAGAACCCTGGCTTCCCGGAGCCGCGCGCCCTCTTCTACACGG
  CGCAGATCATCTGCGGCCTGGAGCACCTGCACCAGAGGCGGATCGTCTACCGCGACCTCA
  AGCCCGAGAACGTGCTGCTGGACAATGACGGCAATGTCCGGATCTCTGACCTTGGGCTGG
  CCGTGGAGCTGCTGGACGGACAGAGCAAGACCAAGGGCTACGCAGGGACCCCAGGTTTCA
  TGGCCCCCGAGCTCCTGCAGGGCGAGGAGTACGACTTCTCCGTGGACTACTTTGCCCTGG
  GGGTCACCCTGTATGAGATGATTGCGGCCAGAGGACCCTTCCGAGCCCGTGGAGAGAAGG
  TGGAGAACAAGGAGCTGAAGCACCGGATCATCTCAGAGCCCGTGAAGTACCCTGATAAGT
  TCAGCCAGGCCAGCAAGGACTTCTGCGAGGCGCTGCTGGAGAAGGACCCGGAGAAGCGCC
  TGGGGTTCAGAGATGAGACCTGCGACAAGCTCCGTGCCCACCCCCTCTTCAAGGACCTTA
  ACTGGAGGCAGCTGGAGGCTGGGATGCTGATGCCCCCTTTCATCCCAGACTCCAAAACTG
  TCTACGCAAAGGATATTCAGGACGTGGGTGCCTTTTCCACCGTCAAAGGTGTGGCCTTTG
  ACAAAACAGACACAGAATTCTTTCAGGAATTTGCCACTGGCAACTGCCCCATCCCCTGGC
  AGGAGGAGATGATCGAGACGGGCATCTTTGGCGAGCTGAACGTGTGGCGCTCGGACGGTC
  AGATGCCGGACGACATGAAGGGCATCTCCGGGGGCTCCAGCTCCTCGTCCAAGTCAGGGA
  TGTGTCTGGTTTCCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002929 unedited
  TCAGGCCTGCTCTGTCTGTGCACGCTCCCAACTTGGCCTCGGCTGGATGGGCCCTCACGC
  CTGAAGCGGGCAGGAAGCTCCGGGATGGATTTCGGGTCTTTGGAGACCGTGGTGGCCAAC
  TCTGCCTTCATCGCCGCCCGAGGCAGCTTTGACGGCAGCAGCTCCCAACCCTCCCGGGAC
  AAGAAGTACCTGGCCAAGCTCAAGCTGCCCCCGCTGTCCAAGTGTGAGTCCCTCCGCGAC
  AGCCTCAGCCTGGAGTTTGAGAGTGTGTGCTTGGAGCAGCCCATCGGCAAGAAGCTCTTT
  CAGCAGTTCCTACAATCGGCAGAGAAGCACCTGCCGGCCCTGGAGCTCTGGAAAGACATC
  GAGGACTATGACACGGCAGACAATGACCTCCAGCCACAGAAGGCCCAGACCATCCTGGCC
  CAGTACCTGGACCCCCAGGCCAAACTCTTCTGCAGCTTCCTGGATGAGGGGATAGTGGCG
  AAGTTTAAGGAGGGGCCTGTGGAGATCCAGGACGGGCTCTTCCAGCCCCTGCTGCAGGCC
  ACCCTGGCACACCTGGGCCAAGCCCCCTTCCAGGAGTACCTGGGCAGCCTGTACTTCCTG
  AGGTTCCTGCAGTGGAAGTGGCTGGAAGCCCAGCCCATGGGGGAGGACTGGTTCCTGGAC
  TTCAAGGTCCTGGGGAAAGGGGGCTTCGGGGAGGTGTCGGCCTGGCCGATGAAAGCGACC
  GGCAAGCTGTATGCCTGCAAAAAGCTGAACAAGAAGCCGCTGAAGAAAAAGAAAGGCTAC
  CAGGGTGCTATGGTGGAGAAGAAGATTCTGATGAAAGTCCACAGCAGGTTCATCGTGTCT
  CTGGCCTATGCGTTTTAAACCAAACCAACCCCGTCCGGTGATGACATCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002929 unedited
  GGGCCATTGGNGATGGCACTTCCAGGNCCAGNAAAGCACTGGGNGAGGGTCACAGGGATG
  CCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGACTAGGAAACC
  AGACACATCCCTGACTTGGACGAGGAGCTGGAGCCCCCGGAGATGCCCTTCATGTCGTCC
  GGCATCTGACCGTCCGAGCGCCACACGTTCAGCTCGCCAAAGATGCCCGTCTCGATCATC
  TCCTCCTGCCAGGGGATGGGGCAGTTGCCAGTGGCAAATTCCTGAAAGAATTCTGTGTCT
  GTTTTGTCAAAGGCCACACCTTTGACGGTGGAAAAGGCACCCACGTCCTGAATATCCTTT
  GCGTAGACAGTTTTGGAGTCTGGGATGAAAGGGGGCATCAGCATCCCAGCCTCCAGCTGC
  CTCCAGTTAAGGTCCTTGAAGAGGGGGTGGGCACGGAGCTTGTCGCAGGTCTCATCTCTG
  AACCCCAGGCGCTTCTCCGGGTCCTTCTCCAGCAGCGCCTCGCAGAAGTCCTTGCTGGCC
  TGGCTGAACTTATCAGGGTACTTCACGGGCTCTGAGATGATCCGGTGCTTCAGCTCCTTG
  TTCTCCACCTTCTCTCCACGGGCTCGGAAGGGTCCTCTGGCCGCAATCATCTCATACAGG
  GTGACCCCCAGGGCAAAGTAGTCCACGGAGAAGTCGTACTCCTCGCCCTGCAGGAGCTCG
  GNGGCCATGAAACCTGNGGTCCCTGCGTAGCCCTTGGTCTTGCTCTGTCCGTCCAGCAGC
  TCCACGGCCAGCCCCAAGTCAGAGATCCGGACATTGCCGTCATTGTCCAGCAGCACGTTC
  TCGGGCTTGAGGTCGCGGTAGACGATCCGCCTCTGGTGCAAGTGCTCCAGGCCGCAAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_002929
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002929.2, NP_002920.1
• RefSeq Size: 2100 bp
• RefSeq ORF: 1692 bp
• Locus ID: 6011
• UniProt ID: Q15835
• Cytogenetics: 13q34
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]