5HT3A receptor (HTR3A) (NM_000869) Human Untagged Clone

CAT#: SC122578

HTR3A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 3A (HTR3A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 5HT3A receptor
• Synonyms: 5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000869 edited
  GCAAGCTGGCCCTTGGTGGGCCTCGTCCTGAGCACTCGGAGGCACTCCTATGCTTGGAAA
  GCTCGCTATGCTGCTGTGGGTCCAGCAGGCGCTGCTCGCCTTGCTCCTCCCCACACTCCT
  GGCACAGGGAGAAGCCAGGAGGAGCCGAAACACCACCAGGCCCGCTCTGCTGAGGCTGTC
  GGATTACCTTTTGACCAACTACAGGAAGGGTGTGCGCCCCGTGAGGGACTGGAGGAAGCC
  AACCACCGTATCCATTGACGTCATTGTCTATGCCATCCTCAACGTGGATGAGAAGAATCA
  GGTGCTGACCACCTACATCTGGTACCGGCAGTACTGGACTGATGAGTTTCTCCAGTGGAA
  CCCTGAGGACTTTGACAACATCACCAAGTTGTCCATCCCCACGGACAGCATCTGGGTCCC
  GGACATTCTCATCAATGAGTTCGTGGATGTGGGGAAGTCTCCAAATATCCCGTACGTGTA
  TATTCGGCATCAAGGCGAAGTTCAGAACTACAAGCCCCTTCAGGTGGTGACTGCCTGTAG
  CCTCGACATCTACAACTTCCCCTTCGATGTCCAGAACTGCTCGCTGACCTTCACCAGTTG
  GCTGCACACCATCCAGGACATCAACATCTCTTTGTGGCGCTTGCCAGAAAAGGTGAAATC
  CGACAGGAGTGTCTTCATGAACCAGGGAGAGTGGGAGTTGCTGGGGGTGCTGCCCTACTT
  TCGGGAGTTCAGCATGGAAAGCAGTAACTACTATGCAGAAATGAAGTTCTATGTGGTCAT
  CCGCCGGCGGCCCCTCTTCTATGTGGTCAGCCTGCTACTGCCCAGCATCTTCCTCATGGT
  CATGGACATCGTGGGCTTCTACCTGCCCCCCAACAGTGGCGAGAGGGTCTCTTTCAAGAT
  TACACTCCTCCTGGGCTACTCGGTCTTCCTGATCATCGTTTCTGACACGCTGCCGGCCAC
  TGCCATCGGCACTCCTCTCATTGGTGTCTACTTTGTGGTGTGCATGGCTCTGCTGGTGAT
  AAGTTTGGCCGAGACCATCTTCATTGTGCGGCTGGTGCACAAGCAAGACCTGCAGCAGCC
  CGTGCCTGCTTGGCTGCGTCACCTGGTTCTGGAGAGAATCGCCTGGCTACTTTGCCTGAG
  GGAGCAGTCAACTTCCCAGAGGCCCCCAGCCACCTCCCAAGCCACCAAGACTGATGACTG
  CTCAGCCATGGGAAACCACTGCAGCCACATGGGAGGACCCCAGGACTTCGAGAAGAGCCC
  GAGGGACAGATGTAGCCCTCCCCCACCACCTCGGGAGGCCTCGCTGGCGGTGTGTGGGCT
  GCTGCAGGAGCTGTCCTCCATCCGGCAATTCCTGGAAAAGCGGGATGAGATCCGAGAGGT
  GGCCCGAGACTGGCTGCGCGTGGGCTCCGTGCTGGACAAGCTGCTATTCCACATTTACCT
  GCTGGCGGTGCTGGCCTACAGCATCACCCTGGTTATGCTCTGGTCCATCTGGCAGTACGC
  TTGAGTGGGTACAGCCCAGTGGAGGAGGGGGTACAGTCCTGGTTAGGTGGGGACAGAGGA
  TTTCTGCTTAGGCCCCTCAGGACCCAGGGAATGCCAGGGACATTTTCAAGACACAGACAA
  AGTCCCGTGCCCTGTTTCCAATGCCAATTCATCTCAGCAATCACAAGCCAAGGTCTGAAC
  CCTTCCACCAAAAACTGGGTGTTCAAGGCCCTTACACCCTTGTCCCACCCCCAGCAGCTC
  ACCATGGCTTTAAAACATGCTCTCTTAGATCAGGAGAAACTCGGGCACTCCCTAAGTCCA
  CTCTAGTTGTGGACTTTTCCCCATTGACCCTCACCTGAATAAGGGACTTTGGAATTCTGC
  TTCTCTTTCACAACTTTGCTTTTAGGTTGAAGGCAAAACCAACTCTCTACTACACAGGCC
  TGATAACTCTGTACGAGGCTTCTCTAACCCCTAGTGTCTTTTTTTTCTTCACCTCACTTG
  TGGCAGCTTCCCTGAACACTCATCCCCCATCAGATGATGGGAGTGGGAAGAATAAAATGC
  AGTGAAACCCTAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000869 unedited
  NNNNCCGAGTACACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGC
  AAGCTGGCCCTTGGTGGGCCTCGTCCTGAGCACCTCGGAGCACTCCTATGCTTGGAAAGC
  TCGCTATGCTGCTGTGGGTCCAGCAGGCGCTGCTCGCCTTGCTCCTCCCCACACTCCTGG
  CACAGGGAGAAGCCAGGAGGAGCCGAAACACCACCAGGCCCGCTCTGCTGAGGCTGTCGG
  ATTACCTTTTGACCAACTACAGGAAGGGTGTGCGCCCCGTGAGGGACTGGAGGAAGCCAA
  CCACCGTATCCATTGACGTCATTGTCTATGCCATCCTCAACGTGGATGAGAAGAATCAGG
  TGCTGACCACCTACATCTGGTACCGGCAGTACTGGACTGATGAGTTTCTCCAGTGGAACC
  CTGAGGACTTTGACAACATCACCAAGTTGTCCATCCCCACGGACAGCATCTGGGTCCCGG
  ACATTCTCATCAATGAGTTCGTGGATGTGGGGAAGTCTCCAAATATCCCGTACGTGTATA
  TTCGGCATCAAGGCGAAGTTCAGAACTACAAGCCCCTTCAGGTGGTGACTGCCTGTAGCC
  TCGACATCTACAACTTCCCCTTCGATGTCCAGAACTGCTCGCTGACCTTCACCAGTTGGC
  TGCACACCATCCAGGACATCAACATCTCTTTGTGGCGCTTGCCAGATAAGGTGAAATCCG
  ACAGGAGTGTCTTCATGAACCAGGGAGAGTGGGAGTTGCTGGGGGTGCTGCCCTACTTTC
  GGGAGTTCAGCATGGAAAGCAGTAACTACTATGCAGAAATGAAGTTCTATGTGGTCATCC
  GCCGGCGGCCCCTN
  
• Restriction Sites: Please inquire
• ACCN: NM_000869
• Insert Size: 2085 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000869.2, NP_000860.1
• RefSeq Size: 2260 bp
• RefSeq ORF: 1437 bp
• Locus ID: 3359
• UniProt ID: P46098
• Cytogenetics: 11q23.2
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), that is missing an internal segment compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.