BDKRB1 (NM_000710) Human Untagged Clone

CAT#: SC122572

BDKRB1 (untagged)-Human bradykinin receptor B1 (BDKRB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BDKRB1
• Synonyms: B1BKR; B1R; BKB1R; BKR1; BRADYB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000710 edited
  CTCAGTCCGTCGGCCCAGACTGAAGTGCAGTGGCACAATCATAGCTCGCTGCAGCCTCGA
  CCTTCCAGGCTTAAACGATTCTCCCACCTCAGCCTCTCGAGTTGCTGGGACCACAGGTCA
  CTGTGCATGGCATCATCCTGGCCCCCTCTAGAGCTCCAATCCTCCAACCAGAGCCAGCTC
  TTCCCTCAAAATGCTACGGCCTGTGACAATGCTCCAGAAGCCTGGGACCTGCTGCACAGA
  GTGCTGCCGACATTTATCATCTCCATCTGTTTCTTCGGCCTCCTAGGGAACCTTTTTGTC
  CTGTTGGTCTTCCTCCTGCCCCGGCGGCAACTGAACGTGGCAGAAATCTACCTGGCCAAC
  CTGGCAGCCTCTGATCTGGTGTTTGTCTTGGGCTTGCCCTTCTGGGCAGAGAATATCTGG
  AACCAGTTTAACTGGCCTTTCGGAGCCCTCCTCTGCCGTGTCATCAACGGGGTCATCAAG
  GCCAATTTGTTCATCAGCATCTTCCTGGTGGTGGCCATCAGCCAGGACCGCTACCGCGTG
  CTGGTGCACCCTATGGCCAGCCGGAGGCAGCAGCGGCGGAGGCAGGCCCGGGTCACCTGC
  GTGCTCATCTGGGTTGTGGGGGGCCTCTTGAGCATCCCCACATTCCTGCTGCGATCCATC
  CAAGCCGTCCCAGATCTGAACATCACCGCCTGCATCCTGCTCCTCCCCCATGAGGCCTGG
  CACTTTGCAAGGATTGTGGAGTTAAATATTCTGGGTTTCCTCCTACCACTGGCTGCGATC
  GTCTTCTTCAACTACCACATCCTGGCCTCCCTGCGAACGCGGGAGGAGGTCAGCAGGACA
  AGGTGCGGGGGCCGCAAGGATAGCAAGACCACAGCGCTGATCCTCACGCTCGTGGTTGCC
  TTCCTGGTCTGCTGGGCCCCTTACCACTTCTTTGCCTTCCTGGAATTCTTATTCCAGGTG
  CAAGCAGTCCGAGGCTGCTTTTGGGAGGACTTCATTGACCTGGGCCTGCAATTGGCCAAC
  TTCTTTGCCTTCACTAACAGCTCCCTGAATCCAGTAATTTATGTCTTTGTGGGCCGGCTC
  TTCAGGACCAAGGTCTGGGAACTTTATAAACAATGCACCCCTAAAAGTCTTGCTCCAATA
  TCTTCATCCCATAGGAAAGAAATCTTCCAACTTTTCTGGCGGAATTAAAACAGCATTGAA
  CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000710 unedited
  GTCATTATTGTATACGACTCATATAGGGCGGCCGCCAGTGTGATGGAATCTGCAGAATTC
  GCCCTTCTGTGCATGGCATCATCCTGGCCCCCTCTAGAGCTCCAATCCTCCAACCAGAGC
  CAGCTCTTCCCTCAAAATGCTACGGCCTGTGACAATGCTCCAGAAGCCTGGGACCTGCTG
  CACAGAGTGCTGCCAACATTTATCATCTCCATCTGTTTCTTCGGCCTCCTAGGGAACCTT
  TTTGTCCTGTTGGTCTTCCTCCTGCCCCGGCGGCAACTGAACGTGGCAGAAATCTACCTG
  GCCAACCTGGCAGCCTCTGATCTGGTGTTTGTCTTGGGCTTGCCCTTCTGGGCAGAGAAT
  ATCTGGAACCAGTTTAACTGGCCTTTCGGAGCCCTCCTCTGCCGTGTCATCAACGGGGTC
  ATCAAGGCCAATTTGTTCATCAGCATCTTCCTGGTGGTGGCCATCAGCCAGGACCGCTAC
  CGCGTGCTGGTGCACCCTATGGCCAGCCGGAGGCAGCAGCGGCGGAGGCAGGCCCGGGTC
  ACCTGCGTGCTCATCTGGGTTGTGGGGGGCCTCTTGAGCATCCCCACATTCCTGCTGCGA
  TCCATCCAAGCCGTCCCAGATCTGAACATCACCGCCTGCATCCTGCTCCTCCCCCATGAG
  GCCTGGCACTTTGCAAGGATTGTGGAGTTAAATATTCTGGGTTTCCTCCTACCACTGGCT
  GCGATCGTCTTCTTCAACTACCACATCCTGGCCTCCCTGCGAACGCGGGAGGAGGTCAGC
  AGGACAAGGTGCGGGGGCCGCAGGATAGCAAGACACAGCGCTGATCCTCACGCTCGTGGT
  TGCCTTCCTGGTCTGCTNGGCCCNCTACCACTTCTTGCCTTCCTGGAATTCTTATTCCCA
  GTGCAAGCAGTCCGAGGCTGCTTTTGTGGAGACTTCATTGACCTGGCCCTGCAATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000710 unedited
  AGGAAAGCACTGGGGNAGGGTCACAGGGCATGCCACCCGGGTATCTGTTCAGGAAAACAG
  CTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGATATCGGTACCGAGCTCGGATCC
  ACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGGTTCAATGCTGTTTTAATTCCG
  CCAGAAAAGTTGGAAGATTTCTTTCCTATGGGATGAAGATATTGGAGCAAGACTTTTAGG
  GGTGCATTGTTTATAAAGTTCCCAGACCTTGGTCCTGAAGAGCCGGCCCACAAAGACATA
  AATTACTGGATTCAGGGAGCTGTTAGTGAAGGCAAAGAAGTTGGCCAATTGCAGGCCCAG
  GTCAATGAAGTCCTCCCAAAAGCAGCCTCGGACTGCTTGCACCTGGAATAAGAATTCCAG
  GAAGGCAAAGAAGTGGTAAGGGGCCCAGCAGACCAGGAAGGCAACCACGAGCGTGAGGAT
  CAGCGCTGTGGTCTTGCTATCCTTGCGGCCCCCGCACCTTGTCCTGCTGACCTCCTCCCG
  CGTTCGCAGGGAGGCCAGGATGTGGTAGTTGAAGAAGACGATCGCAGCCAGTGGTAGGAG
  GAAACCCAGAATATTTAACTCCACAATCCTTGCAAAGTGCCAGGCCTCATGGGGGGGAAG
  GAGCANGGATGCAGGCGGGTGATGTTCATATCTGGGACGGGCTTGAATGGATCGCACAGG
  AGGTGGGNGGGATGCTCAAAAAGCCCCCCACACCCCAAATAGCACGCATGTGAACCCGGG
  CCTGCCTTCGCCGCTGCTGCCTTCGGCTGGCCATAGGGTGCACCACCACGCGGGTACGGT
  CCGGGCTGATGGCCACCACCCGAAAATGCTGGATGAACATTGGCCCTTGAAGACACCGTT
  TATAGACCC
  
• Restriction Sites: Please inquire
• ACCN: NM_000710
• Insert Size: 1235 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000710.2, NP_000701.2
• RefSeq Size: 1319 bp
• RefSeq ORF: 1062 bp
• Locus ID: 623
• UniProt ID: P46663
• Cytogenetics: 14q32.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. The protein encoded by this gene belongs to the G-protein coupled receptor 1 family. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. [provided by RefSeq, Aug 2020]