KCNK10 (NM_138317) Human Untagged Clone

CAT#: SC121923

KCNK10 (untagged)-Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNK10
• Synonyms: K2p10.1; PPP1R97; TREK-2; TREK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138317, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAATTTCCAATCGAGACGCCAAGAAAACAGGTGAACTGGGATCCTAAAGTGGCCGTTCCCGCAGCAG
  CACCGGTGTGCCAGCCCAAGAGCGCCACTAACGGGCAACCCCCGGCTCCGGCTCCGACTCCAACTCCGCG
  CCTGTCCATTTCCTCCCGAGCCACAGTGGTAGCCAGGATGGAAGGCACCTCCCAAGGGGGCTTGCAGACC
  GTCATGAAGTGGAAGACGGTGGTTGCCATCTTTGTGGTTGTGGTGGTCTACCTTGTCACTGGCGGTCTTG
  TCTTCCGGGCATTGGAGCAGCCCTTTGAGAGCAGCCAGAAGAATACCATCGCCTTGGAGAAGGCGGAATT
  CCTGCGGGATCATGTCTGTGTGAGCCCCCAGGAGCTGGAGACGTTGATCCAGCATGCTCTTGATGCTGAC
  AATGCGGGAGTCAGTCCAATAGGAAACTCTTCCAACAACAGCAGCCACTGGGACCTCGGCAGTGCCTTTT
  TCTTTGCTGGAACTGTCATTACGACCATAGGGTATGGGAATATTGCTCCGAGCACTGAAGGAGGCAAAAT
  CTTTTGTATTTTATATGCCATCTTTGGAATTCCACTCTTTGGTTTCTTATTGGCTGGAATTGGAGACCAA
  CTTGGAACCATCTTTGGGAAAAGCATTGCAAGAGTGGAGAAGGTCTTTCGAAAAAAGCAAGTGAGTCAGA
  CCAAGATCCGGGTCATCTCAACCATCCTGTTCATCTTGGCCGGCTGCATTGTGTTTGTGACGATCCCTGC
  TGTCATCTTTAAGTACATCGAGGGCTGGACGGCCTTGGAGTCCATTTACTTTGTGGTGGTCACTCTGACC
  ACGGTGGGCTTTGGTGATTTTGTGGCAGGGGGAAACGCTGGCATCAATTATCGGGAGTGGTATAAGCCCC
  TAGTGTGGTTTTGGATCCTTGTTGGCCTTGCCTACTTTGCAGCTGTCCTCAGTATGATCGGAGATTGGCT
  ACGGGTTCTGTCCAAAAAGACAAAAGAAGAGGTGGGTGAAATCAAGGCCCATGCGGCAGAGTGGAAGGCC
  AATGTCACGGCTGAGTTCCGGGAGACACGGCGAAGGCTCAGCGTGGAGATCCACGATAAGCTGCAGCGGG
  CGGCCACCATCCGCAGCATGGAGCGCCGGCGGCTGGGCCTGGACCAGCGGGCCCACTCACTGGACATGCT
  GTCCCCCGAGAAGCGCTCTGTCTTTGCTGCCCTGGACACCGGCCGCTTCAAGGCCTCATCCCAGGAGAGC
  ATCAACAACCGGCCCAACAACCTGCGCCTGAAGGGGCCGGAGCAGCTGAACAAGCATGGGCAGGGTGCGT
  CCGAGGACAACATCATCAACAAGTTCGGGTCCACCTCCAGACTCACCAAGAGGAAAAACAAGGACCTCAA
  AAAGACCTTGCCCGAGGACGTTCAGAAAATCTACAAGACCTTCCGGAATTACTCCCTGGACGAGGAGAAG
  AAAGAGGAGGAGACGGAAAAGATGTGTAACTCAGACAACTCCAGCACAGCCATGCTGACGGACTGTATCC
  AGCAGCACGCTGAGTTGGAGAACGGAATGATACCCACGGACACCAAAGACCGGGAGCCGGAGAACAACTC
  ATTACTTGAAGACAGAAACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138317 unedited
  GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCAGTGGGCTTCTCCG
  TAGCCCCAGCCTTCAGGCTGCGCAGCCCCTAACCTGCCGGCACCGCTTTTGGGAAGCAGC
  TTGGCTCTTCCATCTCCCCAAGCCTTCCTCTCCATCCTTTCATCCACCCCTCGCCGATCT
  TACTTTTTCTTTTACCCACGGGGCCGCGGCCGGCCACCCCCTGGCCGGTGCANACACCCA
  AGCCCTCTCCAGTTCTCCCCCAAGCTAATATTTTCCCACCTGTCTTTTTCTGGGGTTCCT
  CCCCGAACCAATCCCAGGCTTCCCCATCCTCGGAAAATGGTTTGGTGGACTGGCTAACCG
  AAGCGTGGAAAGCTTGGAGACCTTATTAATAATTGGGGTCCTTTCAATTCTTCCCCTTTT
  GGGCAACGAAACCATTGAAATTTCCAATCGAGACGCCCAAGAAACAAGTGAACTGGGATC
  CCTAAGTGGCCGTTCCCGGCACAGCACCGGTGTTGCCAGCCCCAAGAGCGCCACTAACGG
  GCAACCCCCGGGCTCCGGCTCCGACTCCAAACTCCGCGCCCTGTCCATTTCCCTCCCGAG
  CCCACAGTGGTAGCCCAGGATGGAAGGCACCCTNCCCAAGGGGGCTTGCAGAACCGTCAT
  GGAAGTGGGAAGAACGGTGGTTGCCATCCTTTGTGGTTGTGGTGGTCTACCTTGTCACTG
  GCGGTCTTTGTCTTCCGGCATTGGANCAGCCCTTTGAAAGCAGCCCGAAGAATACCTTCC
  GCCTTGGAGAAGAGCGGAATTTCCGCGGGAAACGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138317 unedited
  CAACTCAACGTGCTGCTGGATACAGTCCGTCAGCATGGCTGTGCTGGAGTTGNNTGAGTT
  ACACATCTTTTCCGTCTCCTCCTCTTTCTTCTCCTCGTCCAGGGAGTAATTCCGGAAGGT
  CTTGTAGATTTTCTGAACGTCCTCGGGCAAGGTCTTTTTGAGGTCCTTGTTTTTCCTCTT
  GGTGAGTCTGGAGGTGGACCCGAACTTGTTGATGATGTTGTCCTCGGACGCACCCTGCCC
  ATGCTTGTTCAGCTGCTCCGGCCCCTTCAGGCGCAGGTTGTTGGGCCGGTTGTTGATGCT
  CTCCTGGGATGAGGCCTTGAAGCGGCCGGTGTCCAGGGCAGCAAAGACAGAGCGCTTCTC
  GGGGGACAGCATGTCCAGTGAGTGGGCCCGCTGGTCCAGGCCCAGCCGCCGGCGCTCCAT
  GCTGCGGATGGTGGCCGCCCGCTGCAGCTTATCGTGGATCTCCACGCTGAGCCTTCGCCG
  TGTCTCCCGGAACTCAGCCGTGACATTGGCCTTCCACTCTGCCGCATGGGCCTTGATTTC
  ACCCACCTCTTCTTTTGTCTTTTTGGACAGAACCCGTAGCCAATCTCCGATCATACTGAG
  GACAGCTGCAAAGTAGGCAAGGCCAACAAGGATCCAAAACCACACTAGGNGCTTATACCA
  CTCCCGATAATTGATGCCAGCGTTTCCCCTTGCCACAAATCACCAAAGCCCACCGTGGTC
  AGAGTGACCACCACANAATAAATTGACTTCAAAGCCGTCCAGGCCCTCATGTACTTAAGA
  TGACCACCGGTTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138317
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138317.1, NP_612190.1
• RefSeq Size: 2484 bp
• RefSeq ORF: 1632 bp
• Locus ID: 54207
• UniProt ID: P57789
• Cytogenetics: 14q31.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (2, also known as TREK2 splice variant c) has a different 5' terminal exon compared to transcript variant 1, and encodes a slightly longer isoform (2) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.