UMPS (NM_000373) Human Untagged Clone

CAT#: SC119938

UMPS (untagged)-Human uridine monophosphate synthetase (UMPS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UMPS
• Synonyms: OPRT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119938 sequence for NM_000373 edited (data generated by NextGen Sequencing)
  ATGGCGGTCGCTCGTGCAGCTTTGGGGCCATTGGTGACGGGTCTGTACGACGTGCAGGCT
  TTCAAGTTTGGGGACTTCGTGCTGAAGAGCGGGCTTTCCTCCCCCATCTACATCGATCTG
  CGGGGCATCGTGTCTCGACCGCGTCTTCTGAGTCAGGTTGCAGATATTTTATTCCAAACT
  GCCCAAAATGCAGGCATCAGTTTTGACACCGTGTGTGGAGTGCCTTATACAGCTTTGCCA
  TTGGCTACAGTTATCTGTTCAACCAATCAAATTCCAATGCTTATTAGAAGGAAAGAAACA
  AAGGATTATGGAACTAAGCGTCTTGTAGAAGGAACTATTAATCCAGGAGAAACCTGTTTA
  ATCATTGAAGATGTTGTCACCAGTGGATCTAGTGTTTTGGAAACTGTTGAGGTTCTTCAG
  AAGGAGGGCTTGAAGGTCACTGATGCCATAGTGCTGTTGGACAGAGAGCAGGGAGGCAAG
  GACAAGTTGCAGGCGCACGGGATCCGCCTCCACTCAGTGTGTACATTGTCCAAAATGCTG
  GAGATTCTCGAGCAGCAGAAAAAAGTTGATGCTGAGACAGTTGGGAGAGTGAAGAGGTTT
  ATTCAGGAGAATGTCTTTGTGGCAGCGAATCATAATGGTTCTCCCCTTTCTATAAAGGAA
  GCACCCAAAGAACTCAGCTTCGGTGCACGTGCAGAGCTGCCCAGGATCCACCCAGTTGCA
  TCGAAGCTTCTCAGGCTTATGCAAAAGAAGGAGACCAATCTGTGTCTATCTGCTGATGTT
  TCACTGGCCAGAGAGCTGTTGCAGCTAGCAGATGCTTTAGGACCTAGTATCTGCATGCTG
  AAGACTCATGTAGATATTTTGAATGATTTTACTCTGGATGTGATGAAGGAGTTGATAACT
  CTGGCAAAATGCCATGAGTTCTTGATATTTGAAGACCGGAAGTTTGCAGATATAGGAAAC
  ACAGTGAAAAAGCAGTATGAAGGAGGTATCTTTAAAATAGCTTCCTGGGCAGATCTAGTA
  AATGCTCACGTGGTGCCAGGCTCAGGAGTTGTGAAAGGCCTGCAAGAAGTGGGCCTGCCT
  TTGCATCGGGGGTGCCTCCTTATTGCGGAAATGAGCTCCACCGGCTCCCTGGCCACTGGG
  GACTACACTAGAGCAGCGGTTAGAATGGCTGAGGAGCACTCTGAATTTGTTGTTGGTTTT
  ATTTCTGGCTCCCGAGTAAGCATGAAACCAGAATTTCTTCACTTGACTCCAGGAGTTCAG
  TTGGAAGCAGGAGGAGATAATCTTGGCCAACAGTACAATAGCCCACAAGAAGTTATTGGC
  AAACGAGGTTCCGATATCATCATTGTAGGTCGTGGCATAATCTCAGCAGCTGATCGTCTG
  GAAGCAGCAGAGATGTACAGAAAAGCTGCTTGGGAAGCGTATTTGAGTAGACTTGGTGTT
  TGA
  
  Clone variation with respect to NM_000373.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000373 unedited
  GTCAGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGCCTCGTGCCGA
  ATTCGGCACGAGGAGGCAGCGCGCGACAATGGCGGTCGCTCGTGCAGCTTTGGGGCCATT
  GGTGACGGGTCTGTACGACGTGCAGGCTTTCAAGTTTGGGGACTTCGTGCTGAAGAGCGG
  GCTTTCCTCCCCCATCTACATCGATCTGCGGGGCATCGTGTCTCGACCGCGTCTTCTGAG
  TCAGGTTGCAGATATTTTATTCCAAACTGCCCAAAATGCAGGCATCAGTTTTGACACCGT
  GTGTGGAGTGCCTTATACAGCTTTGCCATTGGCTACAGTTATCTGTTCAACCAATCAAAT
  TCCAATGCTTATTAGAAGGAAAGAAACAAAGGATTATGGAACTAAGCGTCTTGTAGAAGG
  AACTATTAATCCAGGAGAAACCTGTTTAATCATTGAAGATGTTGTCACCAGTGGATCTAG
  TGTTTTGGAAACTGTTGAGGTTCTTCAGAAGCTGGGCTTGAAGGTCACTGATGCCATAGT
  GCTGTTGGACAGAGAGCAGGGAGGCAAGGACAAGTTGCAGGCGCACGGGATCCGCCTCCA
  CTCAGTGTGTACATTGTCCAAAATGCTGGAGATTCTCGAGCAGCAGAAAAAAGTTGATGC
  TGAGACAGTTGGGAGAGTGAAGAGGTTTATTCAGGAGAATGTCTTTGTGCCAGCGAATCA
  TAATGGTTCTCCCCTTTCTATAAAGGAAGCACCCCAAAGAACTCAGCTTTCGTTGCACGT
  GCCAAAGCTGCCCAGGGATCCACCCCAGTTGCATCGAAAGCTTCTCAAGCTTATGCAAAA
  GGAAGGAGACCAATCTGGGNCTATCTGCTGATGTTTCACTGGCCAGAGNACTGNTGCAGC
  TAACAGATGCTTTAGACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000373 unedited
  GGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCAAGCTTAAAAAT
  GAACTTATTATAGTATCAATGCGGTGATTACAAATTACTCAATATTTCCTAAAGACCATG
  AGAACTCCAGAACCCATTCTTACACAGGCCCTGTGGAAGAATCCAAGCAGGAATAATTGG
  ATTATTTCCAGCCAGTGACTTTCAGGGGACCACATATCTTCAATGTCTTCACATTGTATC
  TGAAAAATGTATCTGAAGCACTCAAACACCAAGTCTACTCAAATACGCTTCCCAAGCAGC
  TTTTCTGTACATCTCTGCTGCTTCCAGACGATCAGCTGCTGAGATTATGCCACGACCTAC
  AATGATGATATCGGAACCTCGTTTGCCAATAACTTCTTGTGGGCTATTGTACTGTTGGCC
  AAGATTATCTCCTCCTGCTTCCAACTGAACTCCTGGAGTCAAGTGAAGAAATTCTGGTTT
  CATGCTTACTCGGGAGCCAGAAATAAAACCAACAACAAATTCAGAGTGCTCCTCAGCCAT
  TCTAACCGCTGCTCTAGTGTAGTCCCCAGTGGCCAGGGAGCCGGTGGAGCTCATTTCCGC
  AATAAGGAGGCACCCCCGATGCAAAGGCAGGCCCACTTCTTGCAGGCCTTTCACAACTCC
  TGAGCCTGGCACCACGTGAGCATTTACTAGATCTGCCCAGGAAGCTATTTTAAAGATACC
  TCCTAAGAAGAAGGGGGAAAAAAGATATTTGCTGATTTTCAACAAAACCTCTAACCCCTC
  TGTCCACCAACCAACTANCATATGAAATNAATCTCCAGTNACATGTTAATGGAGAGATCA
  AAACTCTTCAGGAACTTNTAGTGATATCAGCAGACCCATGGTTCCATCTGTGAAACTGTA
  TCTGTTATTCAGGTAAGTANCTGGGTGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000373
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000373.1, NP_000364.1
• RefSeq Size: 2244 bp
• RefSeq ORF: 1443 bp
• Locus ID: 7372
• UniProt ID: P11172
• Cytogenetics: 3q21.2
• Domains: Pribosyltran, OMPdecase
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
• Gene Summary: This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.