Fibrinogen gamma chain (FGG) (NM_000509) Human Untagged Clone

CAT#: SC119829

FGG (untagged)-Human fibrinogen gamma chain (FGG), transcript variant gamma-A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fibrinogen gamma chain
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119829 sequence for NM_000509 edited (data generated by NextGen Sequencing)
  ATGAGTTGGTCCTTGCACCCCCGGAATTTAATTCTCTACTTCTATGCTCTTTTATTTCTC
  TCTTCAACATGTGTAGCATATGTTGCTACCAGAGACAACTGCTGCATCTTAGATGAAAGA
  TTCGGTAGTTATTGTCCAACTACCTGTGGCATTGCAGATTTCCTGTCTACTTATCAAACC
  AAAGTAGACAAGGATCTACAGTCTTTGGAAGACATCTTACATCAAGTTGAAAACAAAACA
  TCAGAAGTCAAACAGCTGATAAAAGCAATCCAACTCACTTATAATCCTGATGAATCATCA
  AAACCAAATATGATAGACGCTGCTACTTTGAAGTCCAGGAAAATGTTAGAAGAAATTATG
  AAATATGAAGCATCGATTTTAACACATGACTCAAGTATTCGATATTTGCAGGAAATATAT
  AATTCAAATAATCAAAAGATTGTTAACCTGAAAGAGAAGGTAGCCCAGCTTGAAGCACAG
  TGCCAGGAACCTTGCAAAGACACGGTGCAAATCCATGATATCACTGGGAAAGATTGTCAA
  GACATTGCCAATAAGGGAGCTAAACAGAGCGGGCTTTACTTTATTAAACCTCTGAAAGCT
  AACCAGCAATTCTTAGTCTACTGTGAAATCGATGGGTCTGGAAATGGATGGACTGTGTTT
  CAGAAGAGACTTGATGGCAGTGTAGATTTCAAGAAAAACTGGATTCAATATAAAGAAGGA
  TTTGGACATCTGTCTCCTACTGGCACAACAGAATTTTGGCTGGGAAATGAGAAGATTCAT
  TTGATAAGCACACAGTCTGCCATCCCATATGCATTAAGAGTGGAACTGGAAGACTGGAAT
  GGCAGAACCAGTACTGCAGACTATGCCATGTTCAAGGTGGGACCTGAAGCTGACAAGTAC
  CGCCTAACATATGCCTACTTCGCTGGTGGGGATGCTGGAGATGCCTTTGATGGCTTTGAT
  TTTGGCGATGATCCTAGTGACAAGTTTTTCACATCCCATAATGGCATGCAGTTCAGTACC
  TGGGACAATGACAATGATAAGTTTGAAGGCAACTGTGCTGAACAGGATGGATCTGGTTGG
  TGGATGAACAAGTGTCACGCTGGCCATCTCAATGGAGTTTATTACCAAGGTGGCACTTAC
  TCAAAAGCATCTACTCCTAATGGTTATGATAATGGCATTATTTGGGCCACTTGGAAAACC
  CGGTGGTATTCCATGAAGAAAACCACTATGAAGATAATCCCATTCAACAGACTCACAATT
  GGAGAAGGACAGCAACACCACCTGGGGGGAGCCAAACAGGCTGGAGACGTTTAA
  
  Clone variation with respect to NM_000509.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000509 unedited
  GCGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACCAGGCTCGGAGC
  TCCGGGCACTCAGACATCATGAGTTGGTCCTTGCACCCCCGGAATTTAATTCTCTACTTC
  TATGCTCTTTTATTTCTCTCTTCAACATGTGTAGCATATGTTGCTACCAGAGACAACTGC
  TGCATCTTAGATGAAAGATTCGGTAGTTATTGTCCAACTACCTGTGGCATTGCAGATTTC
  CTGTCTACTTATCAAACCAAAGTAGACAAGGATCTACAGTCTTTGGAAGACATCTTACAT
  CAAGTTGAAAACAAAACATCAGAAGTCAAACAGCTGATAAAAGCAATCCAACTCACTTAT
  AATCCTGATGAATCATCAAAACCAAATATGATAGACGCTGCTACTTTGAAGTCCAGGAAA
  ATGTTAGAAGAAATTATGAAATATGAAGCATCGATTTTAACACATGACTCAAGTATTCGA
  TATTTGCAGGAAATATATAATTCAAATAATCAAAAGATTGTTAACCTGAAAGAGAAGGTA
  GCCCAGCTTGAAGCACAGTGCCAGGAACCTTGCAAAGACACGGTGCAAATCCATGATATC
  ACTGGGAAAGATTGTCAAGACATTGCCAATAAGGGAGCTAAACAGAGCGGGCTTTACTTT
  ATTAAACCTCTGANAGCTAACCAGCAATTCTTAGTCTACTGTGAAATCGATGGGTCTGGA
  AATGGATGGACTGTGTTTCAGAGAGACTTGATGGCAGTGTAGATTTCAAGAAAAACTGNG
  ATTCATATANAGAAGGATTTGGACATCTGTCTCCTACTGGCACAACAGAATTTTGGCTGG
  GAAATGAGAAGATTCATTGATAGCACACAGTCTGCCTCCCATATGCATTTAGAGTGGACT
  GGNAGATGGNATGGCAGACCAGTACTGCGNCTATTGCCATGTTCAGGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000509 unedited
  NNNNTTTTTGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTT
  TTAGTCTTAGGAAACAAAGGTTTATTGAAATGCATGTAGATAAATTATCATCAGCATAAA
  ACTGTTATGGAGTTTTCAACATGGGGTCTTTTGCTCTTAAAATGAAGTGAAGCTTTGCAA
  GTCCATTGTCCAATAGGAAAAATATTATATCTCTCTGTTCAGATAAAGTCCTTTAAAAAA
  GTAAATCTCTTTTGAAACGGTCTTTTAAACGTCTCCAGCCTGTTTGGCTCCCCCCAGGTG
  GTGTTGCTGTCCTTCTCCAATTGTGAGTCTGTTGAATGGGATTATCTTCATAGTGGTTTT
  CTTCATGGAATACCACCGGGTTTTCCAAGTGGCCCAAATAATGCCATTATCATAACCATT
  AGGAGTAGATGCTTTTGAGTAAGTGCCACCTTGGTAATAAACTCCATTGAGATGGCCAGC
  GTGACACTTGTTCATCCACCAACCAGATCCATCCTGTTCAGCACAGTTGCCTTCAAACTT
  ATCATTGTCATTGTCCCAGGTACTGAACTGCATGCCATTATGGGATGTGAAAAACTTGTC
  ACTAGGATCATCGCCAAAATCAAAGCCATCAAAGGCATCTCCAGCATCCCCACCAGCGAA
  GTAGGCATATGTTAGGCGGTACTTGTCAGCTTCAGGTCCCACCTTGAACATGGCATAGTC
  TGCAGTACTGGTTCTGCCATTCCAGTCTTCCAGTTCCACTCTTAATGCATATNGGGATGG
  CANGACTGGTGTGCTTATCAAATGAATCTTCTCATTTCCAGCCAAAATNCTGTTGTGCCA
  GTAGGAAACAGATGTCCCAATCCTTNCTTATATTGAAATCCAGTTTTTCTTGAAATCTAC
  ACTGCCCCATCAGTCTCTTCTGAAACACAGNTCCATNCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000509
• Insert Size: 1570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000509.4, NP_000500.2
• RefSeq Size: 1665 bp
• RefSeq ORF: 1314 bp
• Locus ID: 2266
• UniProt ID: P02679
• Cytogenetics: 4q32.1
• Domains: FBG
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (gamma-A) is the predominant transcript and is shorter than variant gamma-B. Variant gamma-A encodes isoform gamma-A, which is shorter and has a distinct C-terminus compared to isoform gamma-B.