HTR1D (NM_000864) Human Untagged Clone

CAT#: SC119613

HTR1D (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 1D (HTR1D)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HTR1D
• Synonyms: 5-HT1D; HT1DA; HTR1DA; HTRL; RDC4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000864, RT-PCR generated
  ATGTCCCCACTGAACCAGTCAGCAGAAGGCCTTCCCCAGGAGGCCTCCAACAGATCCCTG
  AATGCCACAGAAACCTCAGAGGCTTGGGATCCCAGGACCCTCCAGGCGCTCAAGATCTCC
  CTTGCCGTGGTCCTTTCCGTCATCACACTGGCCACAGTCCTCTCCAATGCCTTTGTACTC
  ACCACCATCTTACTCACCAGGAAGCTCCACACCCCTGCCAACTACCTGATTGGCTCCCTG
  GCCACCACCGACCTCTTGGTTTCCATCTTGGTAATGCCCATCAGCATCGCCTATACCATC
  ACCCACACCTGGAACTTTGGCCAAATCTTGTGTGACATCTGGCTGTCCTCTGACATCACG
  TGCTGCACAGCCTCCATCCTGCATCTCTGTGTCATTGCTCTGGACAGGTACTGGGCAATC
  ACAGATGCCCTGGAATACAGTAAACGCAGGACGGCTGGCCACGCGGCCACCATGATCGCC
  ATTGTCTGGGCCATCTCCATCTGCATCTCCATCCCCCCGCTCTTCTGGCGGCAGGCCAAG
  GCCCAGGAGGAGATGTCGGACTGTCTGGTGAACACCTCTCAGATTTCCTACACCATCTAC
  TCCACCTGTGGGGCCTTCTACATTCCCTCGGTGTTGCTCATCATCCTATATGGCCGGATC
  TACCGGGCTGCCCGGAACCGCATCCTGAATCCACCCTCACTCTATGGGAAGCGCTTCACC
  ACGGCCCACCTCATCACAGGCTCTGCCGGGTCCTCGCTCTGCTCGCTCAACTCCAGCCTC
  CATGAGGGGCACTCGCACTCGGCTGGCTCCCCTCTCTTTTTCAACCACGTGAAAATCAAG
  CTTGCTGACAGTGCCCTGGAACGCAAGAGGATTTCTGCTGCTCGAGAAAGGAAAGCCACT
  AAAATCCTGGGCATCATTCTGGGGGCCTTTATCATCTGCTGGCTGCCCTTCTTCGTGGTG
  TCTCTGGTCCTCCCCATCTGCCGGGACTCCTGCTGGATCCACCCGGCGCTCTTTGACTTC
  TTCACCTGGCTAGGCTATTTAAACTCCCTCATCAATCCAATAATCTACACTGTGTTTAAT
  GAAGAGTTTCGGCAAGCTTTTCAGAAAATTGTCCCTTTCCGGAAGGCCTCCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000864 unedited
  GGAACTCGAGATTTGTTAAACGACTTACTATAGGCGGCCGCGNAATCANATCTGGTACCG
  AGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTGAGGTCTGTGGGA
  AGAGAGAGCCACCTAGCATGTCCCCACTGAACCAGTCAGCAGAAGGCCTTCCCCAGGAGG
  CCTCCAACAGATCCCTGAATGCCACAGAAACCTCAGAGGCTTGGGATCCCAGGACCCTCC
  AGGCGCTCAAGATCTCCCTTGCCGTGGTCCTTTCCGTCATCACACTGGCCACAGTCCTCT
  CCAATGCCTTTGTACTCACCACCATCTTACTCACCAGGAAGCTCCACACCCCTGCCAACT
  ACCTGATTGGCTCCCTGGCCACCACCGACCTCTTGGTTTCCATCTTGGTAATGCCCATCA
  GCATCGCCTATACCATCACCCACACCTGGAACTTTGGCCAAATCTTGTGTGACATCTGGC
  TGTCCTCTGACATCACGTGCTGCACAGCCTCCATCCTGCATCTCTGTGTCATTGCTCTGG
  ACAGGTACTGGGCAATCACAGATGCCCTGGAATACAGTAAACGCAGGACGGCTGGCCACG
  CGGCCACCATGATCGCCATTGTCTGGGCCATCTCCATCTGCATCTCCATCCCCCCGCTCT
  TCTGGCGGCAGGCCAAGGCCCAGGAGGAGATGTCGGACTGTCTGGTGAACACCTCTCAGA
  TTTCCTACACCATCTACTCCACCTGTGGGGCCTTCTACATTCCCTCGGTGTTGCTCATCA
  TCCTATATGGGCCGGATCTACCGGGCTGCCCGGAACCGCATCCTGAATCCACCCTCACTC
  TATGGGGAGCGCTTCACCACGGCCCACCTCATCACAGGCTCTGCCGGGTACTCGCTCTGC
  TCGCTCACTCCAGCCTCCATGAGGGGCACT
  
• Restriction Sites: Please inquire
• ACCN: NM_000864
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000864.3, NP_000855.1
• RefSeq Size: 2855 bp
• RefSeq ORF: 1134 bp
• Locus ID: 3352
• UniProt ID: P28221
• Cytogenetics: 1p36.12
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.[UniProtKB/Swiss-Prot Function]