Thrombin Receptor (F2R) (NM_001992) Human Untagged Clone

CAT#: SC118924

F2R (untagged)-Human coagulation factor II (thrombin) receptor (F2R)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thrombin Receptor
• Synonyms: CF2R; HTR; PAR-1; PAR1; TR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118924 sequence for NM_001992 edited (data generated by NextGen Sequencing)
  ATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTG
  TCTGCCCGCACCCGGGCCCGCAGGCCAGAATCAAAAGCAACAAATGCCACCTTAGATCCC
  CGGTCATTTCTTCTCAGGAACCCCAATGATAAATATGAACCATTTTGGGAGGATGAGGAG
  AAAAATGAAAGTGGGTTAACTGAATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTT
  CAAAAACAACTTCCTGCATTCATCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGG
  CTGACACTCTTTGTCCCATCTGTGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAAC
  ATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTAC
  ATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGC
  TATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCA
  GCATTTTACTGTAACATGTACGCCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGG
  TTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCC
  TTCACTTGTCTGGCCATCTGGGCTTTGGCCATCGCAGGGGTAGTGCCTCTGCTCCTCAAG
  GAGCAAACCATCCAGGTGCCCGGGCTCAACATCACTACCTGTCATGATGTGCTCAATGAA
  ACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTCTGCTGTCTTCTTTTTT
  GTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCGATGTCTTAGCTCTTCC
  GCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTCAGCTGCTGTTTTCTGC
  ATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGCGCATTACTCATTCCTT
  TCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCTCTGTGTCTGTGTCAGC
  AGCATAAGCTGCTGCATCGACCCCCTAATTTACTATTACGCTTCCTCTGAGTGCCAGAGG
  TACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAGCAGTTATAACAGCAGT
  GGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCTGAATAACAGCATATAC
  AAAAAGCTGTTAACTTAG
  
  Clone variation with respect to NM_001992.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001992 unedited
  GGCTCCGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAGCGCTCGCCGAGGG
  TCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGC
  TCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCC
  TCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGG
  CCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAG
  AATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATG
  ATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACA
  GATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAG
  AAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACA
  CCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGA
  AAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGCCCACGGCAGATGTGC
  TGGTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGCCAGT
  TTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTACCATGTACGCCCTCT
  ATCTTGCTCATGACAGTCATAGGCATTGACCCGGTTCCTGGCTTGTGGTGTATCCCATGC
  AGNNCCCTTCTCTGGCGGACTCTGGGGAGGGGCTTTCTTTCACTTGGCTGGCCATCTGGG
  CCTTTGGCCTCGCCAGGGTAGGGCCTCTGCTCCTAAGGAGCAACCATTCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001992 unedited
  TGACCGCGGCCGCAATTTANAGTCGAGTTTTTTTTTTTTTTTTTTATTTTTATTTTTTGA
  GATGGAGTCTTGCTCTGTTGCCCAGGCTGGAGCACAGTGGCGCGATCTCGGCTCACTACA
  AGGTCCGCCTCCTGGGTTCACGCCAGTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTA
  CAGGTGCCTGCCACCACGCCCGGCTAATTTTTTTTGCATTTTTAGTAGAGACGGGTTTCA
  CCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCCGCCTCAGCCTCCC
  AAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGGCCTGCTCTTTTAAATAAACATT
  TGCCTTGCAACTTTCTATTTTGTTTAAAATGCTACCATTTTTTCGTAAGATAAGAGATAT
  GTTTCAACCTAATTTCCAAATTACTTTGTGAATTCCATGAAGATTTTCTATACTTGATAC
  TTAGGAGGTTAAAAAATGCTTTTAAAGTCTCATTAAGTCTTAAGTAAATGGAAATTATAG
  AATAATTTTCCTAGATTTCAATGGCAATTCTATTTTCTTCTGTTATTTCTAAGCAGACTG
  ACATGATTGATTCTTAAAAGAAAACCTGATTTGAGCAATCTATTGAGCAAAACATTTGTA
  AATAAATAGTGCCCAATTAAATCAGACTTATTTTAAAAAATATATGTCCTGTACTGCATT
  GNTTTCATTAAGTTANNTACCACAGAAATAGTACCCCAATGTTCAAACTTCCTTAGCTGA
  CAGGCCTTGGTTTGTGTGAACATATTTATTCACATACATCACACTCTATGCATGATCTAC
  TTTGCTCTTGAGGGGCAGGCTCTGTCTTATCCACTCTGTAACTCAGCACCTATTGGATGG
  CCCAGCGCATATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001992
• Insert Size: 3870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001992.2, NP_001983.1
• RefSeq Size: 3592 bp
• RefSeq ORF: 1278 bp
• Locus ID: 2149
• UniProt ID: P25116
• Cytogenetics: 5q13.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Complement and coagulation cascades, Endocytosis, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).