FGR (NM_005248) Human Untagged Clone

CAT#: SC116870

FGR (untagged)-Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGR
• Synonyms: c-fgr; c-src2; p55-Fgr; p55c-fgr; p58-Fgr; p58c-fgr; SRC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005248 edited
  ATGGGCTGTGTGTTCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGC
  CTGGAAGGGGACTTCAGAAGCTACGGGGCAGCAGACCACTATGGGCCTGACCCCACTAAG
  GCCCGGCCTGCATCCTCATTTGCCCACATCCCCAACTACAGCAACTTCTCCTCTCAGGCC
  ATCAACCCTGGCTTCCTTGATAGTGGCACCATCAGGGGTGTGTCAGGGATTGGGGTGACC
  CTGTTCATTGCCCTGTATGACTATGAGGCTCGAACTGAGGATGACCTCACCTTCACCAAG
  GGCGAGAAGTTCCACATCCTGAACAATACTGAAGGTGACTGGTGGGAGGCTCGGTCTCTC
  AGCTCCGGAAAAACTGGCTGCATTCCCAGCAACTACGTGGCCCCTGTTGACTCAATCCAA
  GCTGAAGAGTGGTACTTTGGAAAGATTGGGAGAAAGGATGCAGAGAGGCAGCTGCTTTCA
  CCAGGCAACCCCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCACCAAAGGTGCCTAC
  TCCCTGTCCATCCGGGACTGGGATCAGACCAGAGGCGATCATGTGAAGCATTACAAGATC
  CGCAAACTGGACATGGGCGGCTACTACATCACCACACGGGTTCAGTTCAACTCGGTGCAG
  GAGCTGGTGCAGCACTACATGGAGGTGAATGACGGGCTGTGCAACCTGCTCATCGCGCCC
  TGCACCATCATGAAGCCGCAGACGCTGGGCCTGGCCAAGGACGCCTGGGAGATCAGCCGC
  AGCTCCATCACGCTGGAGCGCCGGCTGGGCACCGGCTGCTTCGGGGATGTGTGGCTGGGC
  ACGTGGAACGGCAGCACTAAGGTGGCGGTGAAGACGCTGAAGCCGGGCACCATGTCCCCG
  AAGGCCTTCCTGGAGGAGGCGCAGGTCATGAAGCTGCTGCGGCACGACAAGCTGGTGCAG
  CTGTACGCCGTGGTGTCGGAGGAGCCCATCTACATCGTGACCGAGTTCATGTGTCACGGC
  AGCTTGCTGGATTTTCTCAAGAACCCAGAGGGCCAGGATTTGAGGCTGCCCCAATTGGTG
  GACATGGCAGCCCAGGTAGCTGAGGGCATGGCCTACATGGAACGCATGAACTACATTCAC
  CGCGACCTGAGGGCAGCCAACATCCTGGTTGGGGAGCGGCTGGCGTGCAAGATCGCAGAC
  TTTGGCTTGGCGCGTCTCATCAAGGACGATGAGTACAACCCCTGCCAAGGTTCCAAGTTC
  CCCATCAAGTGGACAGCCCCAGAAGCTGCCCTCTTTGGCAGATTCACCATCAAGTCAGAC
  GTGTGGTCCTTTGGGATCCTGCTCACTGAGCTCATCACCAAGGGCCGAATCCCCTACCCA
  GGCATGAATAAACGGGAAGTGTTGGAACAGGTGGAGCAGGGCTACCACATGCCGTGCCCT
  CCAGGCTGCCCAGCATCCCTGTACGAGGCCATGGAACAGACCTGGCGTCTGGACCCGGAG
  GAGAGGCCTACCTTCGAGTACCTGCAGTCCTTCCTGGAGGACTACTTCACCTCCGCTGAA
  CCACAGTACCAGCCCGGGGATCAGACATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005248 unedited
  TTACGATTTTGTAATACGACTTACTATAGGGCGGCCGCGNAATCGGCACGAGGCCGGCAC
  TCGCGGCTGCTGGAGCCCCGGCTGGCTCACCCCGGGGCCGGGCAGAATTGGGCTCCAGGT
  CTCTGACCCCTCCCAAGGATCATGCCGCAGCCCCACTGACCCAGGAGTAGGGGCCTAAGG
  GCAGGGAACCTGGAATGGGCTGTGTGTTCTGCAAGAAATTGGAGCCGGTGGCCACGGCCA
  AGGAGGATGCTGGCCTGGAAGGGGACTTCAGAAGCTACGGGGCAGCAGACCACTATGGGC
  CTGACCCCACTAAGGCCCGGCCTGCATCCTCATTTGCCCACATCCCCAACTACAGCAACT
  TCTCCTCTCAGGCCATCAACCCTGGCTTCCTTGATAGTGGCACCATCAGGGGTGTGTCAG
  GGATTGGGGTGACCCTGTTCATTGCCCTGTATGACTATGAGGCTCGAACTGAGGATGACC
  TCACCTTCACCAAGGGCGAGAAGTTCCACATCCTGAACAATACTGAAGGTGACTGGTGGG
  AGGCTCGGTCTCTCAGCTCCGGAAAAACTGGCTGCATTCCCAGCAACTACGTGGCCCCTG
  TTGACTCAATCCAAGCTGAAGAGTGGTACTTTGGAAAGATTGGGAGAAAGGATGCAGAGA
  GGCAGCTGCTTTCACCAGGCAACCCCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCA
  CCAAAGGTGCCTACTCCCTGTCCATCCGGGACTGGGATCAGACCAGAGGCGATCATGTGA
  AGCATTTACAGATCCGCANACTGGACATGGGCGGCTACTACATCACCACACGGGGTTCAG
  TTCAACTCGGTGGCAGAGCTGGTGCAGCACTACATGGNAGGTGAATGACGGGCTGTGCAA
  CCTGCTCATCGCGCCCTGCACCATCATGAAGCCGCAGACGCTGGNCCTGGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005248 unedited
  TGGACCGCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG
  AGGATTCATATTGGCATTTTACTTATTTACAGAATCAATAAACCAACACATACACACTAT
  TCAGAGAGGGGGGAAGTGCTCTGCAACCTTCTCCCTCAAACCTGGGCCCAGACCCCAGTC
  CTGGACCACTGCATCCACCCAGCAGGAAAGGGGTCCAGCCAAGACTTTTCCTGACTTTGT
  AACTTACAGACACAAGAGAATAGAGGGTAGAAGGGAAATTCTTGGCACCTGGACTAGAGT
  GAGATAAAAGGAGAGTAGGAAAGCAGTGATAGGAGAGAAGTGAGGGAGGTACATACAGTT
  TTATAAATAACTAGACAAGGTCTGAGCACTTTGGGTGGGGATGGAGTGAGAAAGGCTACA
  GGCATGTAGGGGCCTAAGTGGAAAAGGAAGAAATAGTGCTTGGGGCCAGAGCGGATGAGA
  GATCAGCTCTGGGCCTCCTTTTGCCCCATCTGTAAAACAAGTAGGTTGCCCTAGGTGGTG
  TCAGAGCAGCCACGTCCTCGGTGATGCTAGGACTCTATGGGGTTCTAAGCCAGCCTGGGG
  GCTTTGGAAGAACAGCTCTGGGGATTGGCAAGGACTGGTGGCCACCGCCAGAGAGGGTTG
  ATGCCCGGACAGGCTATGTCTGATCCCCGGGCTGGTACTGTGGTTCAGCGGAGGTGAAGT
  AGTCCTCCAGGAGGACTGCAGGTACTNCGAGGTAGGCCTCTCCTCCGGGTCCAGACGCCA
  GGTCTGTTCCATGCCCTCGTCAGGGATGCTGGNCAANCTGGAGGGCACCGCATGTGGGTA
  CCCTGCTTCCACCTGTCCAACACTTCCGTTTTTCATGCCTGGGTAGGGGATTCGCCCCTT
  GGGATGAGCTCAATGAGCCAGATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005248
• Insert Size: 2450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005248.1, NP_005239.1
• RefSeq Size: 2354 bp
• RefSeq ORF: 1590 bp
• Locus ID: 2268
• UniProt ID: P09769
• Cytogenetics: 1p35.3
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway
• Gene Summary: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) initiates at exon M4 and represents the longest transcript. It is expressed in myelomonocytic cells. Variants 1, 2, and 3 encode the same isoform.