Cytokeratin 7 (KRT7) (NM_005556) Human Untagged Clone

CAT#: SC116650

KRT7 (untagged)-Human keratin 7 (KRT7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytokeratin 7
• Synonyms: CK7; K2C7; K7; SCL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116650 sequence for NM_005556 edited (data generated by NextGen Sequencing)
  ATGTCCATCCACTTCAGCTCCCCGGTATTCACCTCGCGCTCAGCCGCCTTCTCGGGCCGC
  GGCGCCCAGGTGCGCCTGAGCTCCGCTCGCCCCGGCGGCCTTGGCAGCAGCAGCCTCTAC
  GGCCTCGGCGCCTCGCGGCCGCGCGTGGCCGTGCGCTCTGCCTATGGGGGCCCGGTGGGC
  GCCGGCATCCGCGAGGTCACCATTAACCAGAGCCTGCTGGCCCCGCTGCGGCTGGACGCC
  GACCCCTCCCTCCAGCGGGTGCGCCAGGAGGAGAGCGAGCAGATCAAGACCCTCAACAAC
  AAGTTTGCCTCCTTCATCGACAAGGTGCGGTTTCTGGAGCAGCAGAACAAGCTGCTGGAG
  ACCAAGTGGACGCTGCTGCAGGAGCAGAAGTCGGCCAAGAGCAGCCGCCTCCCAGACATC
  TTTGAGGCCCAGATTGCTGGCCTTCGGGGTCAGCTTGAGGCACTGCAGGTGGATGGGGGC
  CGCCTGGAGGCGGAGCTGCGGAGCATGCAGGATGTGGTGGAGGACTTCAAGAATAAGTAC
  GAAGATGAAATTAACCGCCGCACAGCTGCTGAGAATGAGTTTGTGGTGCTGAAGAAGGAT
  GTGGATGCTGCCTACATGAGCAAGGTGGAGCTGGAGGCCAAGGTGGATGCCCTGAATGAT
  GAGATCAACTTCCTCAGGACCCTCAATGAGACGGAGTTGACAGAGCTGCAGTCCCAGATC
  TCCGACACATCTGTGGTGCTGTCCATGGACAACAGTCGCTCCCTGGACCTGGACGGCATC
  ATCGCTGAGGTCAAGGCACAGTATGAGGAGATGGCCAAATGCAGCCGGGCTGAGGCTGAA
  GCCTGGTACCAGACCAAGTTTGAGACCCTCCAGGCCCAGGCTGGGAAGCATGGGGACGAC
  CTCCGGAATACCCGGAATGAGATTTCAGAGATGAACCGGGCCATCCAGAGGCTGCAGGCT
  GAGATCGACAACATCAAGAACCAGCGTGCCAAGTTGGAGGCCGCCATTGCCGAGGCTGAG
  GAGCGTGGGGAGCTGGCGCTCAAGGATGCTCGTGCCAAGCAGGAGGAGCTGGAAGCCGCC
  CTGCAGCGGGCCAAGCAGGATATGGCACGGCAGCTGCGTGAGTACCAGGAACTCATGAGC
  GTGAAGCTGGCCCTGGACATCGAGATCGCCACCTACCGCAAGCTGCTGGAGGGCGAGGAG
  AGCCGGTTGGCTGGAGATGGAGTGGGAGCCGTGAATATCTCTGTGATGAATTCCACTGGT
  GGCAGTAGCAGTGGCGGTGGCATTGGGCTGACCCTCGGGGGAACCATGGGCAGCAATGCC
  CTGAGCTTCTCCAGCAGTGCGGGTCCTGGGCTCCTGAAGGCTTATTCCATCCGGACCGCA
  TCCGCCAGTCGCAGGAGTGCCCGCGACTGA
  
  Clone variation with respect to NM_005556.3
  135 a=>g;557 a=>g;798 g=>a;1091 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005556 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCTCCTCCTCGCCCGCCG
  CTAGGTCCATCCCGGCCCAGCCACCATGTCCATCCACTTCAGCTCCCCGGTATTCACCTC
  GCGCTCAGCCGCCTTCTCGGGCCGCGGCGCCCAGGTGCGCCTGAGCTCCGCTCGCCCCGG
  CGGCCTTGGCAGCAGCAGCCTCTACGGCCTCGGCGCCTCGCGGCCGCGCGTGGCCGTGCG
  CTCTGCCTATGGGGGCCCGGTGGGCGCCGGCATCCGCGAGGTCACCATTAACCAGAGCCT
  GCTGGCCCCGCTGCGGCTGGACGCCGACCCCTCCCTCCAGCGGGTGCGCCAGGAGGAGAG
  CGAGCAGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTTCT
  GGAGCAGCAGAACAAGCTGCTGGAGACCAAGTGGACGCTGCTGCAGGAGCAGAAGTCGGC
  CAAGAGCAGCCGCCTCCCAGACATCTTTGAGGCCCAGATTGCTGGCCTTCGGNGTCAGCT
  TGAGGCACTGCAGGTGGATGGGGGCCGCCTGGAGGCGGAGCTGCGGAGCATGCANGATGT
  GGTGGAGGACTTCAAGAAATAGTACGAAGATGAAATTAACCGCCGCACAGCTGCTGAGAA
  TGAGTTTGTTGGTGCTGAAGAAAGGATGTGATGCTGCCTACATGAGCANGGTGGAGCTGG
  GAGCCANGGTGGATGCCCTGATGATGANGATCACTTNCTCAGGACCCTCATGANACAGGA
  GTGACAGAGCTGCAGTCCCAGATCTCCACCATNNTGGGGGGGGGCTGTCCATGGGACACA
  GTCGCTCCCTTGACACTGNACGCATCATNNCNTGAGGTCAGGCACAGATGTGGGAGAATG
  GCCAATGCANCCGGGCTGAGGCTGAGCCTGGACCCAAACAAGTTTGAGACCTCAGNCCAG
  CTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005556 unedited
  GCGCCCTTCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTTCACTCAGGCCTCAAAATGA
  GGCTGCTTTATTGGAAGCTATTCTGACATCACTTTCCAAACTGTCTCACTGTCTTGGGAC
  CAGGCATGGGAGGCAGGGGTGGGAATCTTCTTGTGATTGTGGGTGGTGGCTGGAGGATTG
  GATTGGTGGGAGGCGGCTCATTCGCGGGCACTCCTGCGACTGGCGGATGCGGTCCGGATG
  GAATAATCCTTCAGGAGCCCAGGACCCGCACTGCTGGAGAAGCTCAGGGCATTGCTGCCC
  ATGGTTCCCCCGAGGGTCAGCCCATTGCCACCGCCACTGCTACTGCCACCAGTGGAATTC
  ATCACAGATATATTCACGGCTCCCACTCCATCTCCAGCCAACCGGCTCTCCTCGCCCTCC
  ATAAGTTTGCGGTAGGTGGCGATCTCAATGTCCAGGCCCAGCTTCACGCTCATGAGTTCC
  TGGAACTCACGCAGCTGCCGTGCCTTATCCTGCTTGGCCCGCTGCAGGGCGGCTTCCATC
  CTCTCCTGCTTGGCCCGATCATCCTTGACCGCCACCTCCCACCCCTCCTTATCTTAGACT
  ATGGCGGCCTCCAACTTGGCCCGTTGGTTCTTGATGTTGGCCATCCCACCCTGAGACCTC
  TGGATGGCCCCCTTTATTCTCTGAAATTCCCTTCTCGGGGTATTTCCGGAGGGTCTCCCC
  TTTCCTCCCCAGCCTGGGGCCGGGAAGGTTCTTAAATTTGGTTCTGGTACACATGTTTAA
  CCCTAACCCCGGTTGATTTTGACCCTCTCCTCTTTCTGGGCCCTCGACCTTTACGTTGAT
  GCCCCCCCATCTCCAGCGGCCCTCCTTTTTCTCTGGTCACTCCTCCATATTTGCCAGTAT
  TCCGACACTGCACTCCTTCCCTCTCGTCTTCTGGGGTCTCTTGGATTTCTTTCCTTTTAC
  GGGTCTCCCTTCGGCCCCACTCTCCCTTTGTTTGTGCGCTATCCCCCCTCTCCTCACACC
  ACTTTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005556
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005556.3, NP_005547.3
• RefSeq Size: 1753 bp
• RefSeq ORF: 1410 bp
• Locus ID: 3855
• UniProt ID: P08729
• Cytogenetics: 12q13.13
• Domains: filament
• Protein Families: ES Cell Differentiation/IPS
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]