FOXO4 (NM_005938) Human Untagged Clone

CAT#: SC116424

FOXO4 (untagged)-Human forkhead box O4 (FOXO4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FOXO4
• Synonyms: AFX; AFX1; MLLT7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116424 sequence for NM_005938 edited (data generated by NextGen Sequencing)
  ATGGATCCGGGGAATGAGAATTCAGCCACAGAGGCTGCCGCGATCATAGACCTAGATCCC
  GACTTCGAACCCCAGAGCCGTCCCCGCTCCTGCACCTGGCCCCTTCCCCGACCAGAGATC
  GCTAACCAGCCGTCCGAGCCGCCCGAGGTGGAGCCAGATCTGGGGGAAAAGGTACACACG
  GAGGGGCGCTCAGAGCCGATCCTGTTGCCCTCTCGGCTCCCAGAGCCGGCCGGGGGCCCC
  CAGCCCGGAATCCTGGGGGCTGTAACAGGTCCTCGGAAGGGAGGCTCCCGCCGGAATGCC
  TGGGGAAATCAGTCATATGCAGAACTCATCAGCCAGGCCATTGAAAGCGCCCCGGAGAAG
  CGACTGACACTTGCCCAGATCTACGAGTGGATGGTCCGTACTGTACCCTACTTCAAGGAC
  AAGGGTGACAGCAACAGCTCAGCAGGATGGAAGAACTCGATCCGCCACAACCTGTCCCTG
  CACAGCAAGTTCATCAAGGTTCACAACGAGGCCACCGGCAAAAGCTCTTGGTGGATGCTG
  AACCCTGAGGGAGGCAAGAGCGGCAAAGCCCCCCGCCGCCGGGCCGCCTCCATGGATAGC
  AGCAGCAAGCTGCTCCGGGGCCGCAGTAAAGCCCCCAAGAAGAAACCATCTGTGCTGCCA
  GCTCCACCCGAAGGTGCCACTCCAACGAGCCCTGTCGGCCACTTTGCCAAGTGGTCAGGC
  AGCCCTTGCTCTCGAAACCGTGAAGAAGCCGATATGTGGACCACCTTCCGTCCACGAAGC
  AGTTCAAATGCCAGCAGTGTCAGCACCCGGCTGTCCCCCTTGAGGCCAGAGTCTGAGGTG
  CTGGCGGAGGAAATACCAGCTTCAGTCAGCAGTTATGCAGGGGGTGTCCCTCCCACCCTC
  AATGAAGGTCTAGAGCTGTTAGATGGGCTCAATCTCACCTCTTCCCATTCCCTGCTATCT
  CGGAGTGGTCTCTCTGGCTTCTCTTTGCAGCATCCTGGGGTTACCGGCCCCTTACACACC
  TACAGCAGCTCCCTTTTCAGCCCAGCAGAGGGGCCCCTGTCAGCAGGAGAAGGGTGCTTC
  TCCAGCTCCCAGGCTCTGGAGGCCCTGCTCACCTCTGATACGCCACCACCCCCTGCTGAC
  GTCCTCATGACCCAGGTAGATCCCATTCTGTCCCAGGCTCCGACTCTTCTGTTGCTGGGG
  GGGCTTCCTTCCTCCAGTAAGCTGGCCACGGGCGTCGGCCTGTGTCCCAAGCCCCTAGAG
  GCTCCAGGCCCCAGCAGTCTGGTTCCCACCCTTTCTATGATAGCACCACCTCCAGTCATG
  GCAAGTGCCCCCATCCCCAAGGCTCTGGGGACTCCTGTGCTCACACCCCCTACTGAAGCT
  GCAAGCCAAGACAGAATGCCTCAGGATCTAGATCTTGATATGTATATGGAGAACCTGGAG
  TGTGACATGGATAACATCATCAGTGACCTCATGGATGAGGGCGAGGGACTGGACTTCAAC
  TTTGAGCCAGATCCCTGA
  
  Clone variation with respect to NM_005938.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005938 unedited
  CCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG
  CTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTC
  GGCACGAGAGGTTGCAGAAAAAGTGTCTTCGCTCGGCAGAGGTTACAGGTGGCATCTCAG
  AAAGAGCTTTGAGGCTACAGGCTGTAGTCGGGAAGGGGATCGGAGAACTGTGTGAAGGGA
  CAGCTTAGGGACTAGCGTCCTGGGACTAGGGGGAAGTTCGCGACTTTCTGAAGACTGGCA
  GGAATGTGCCTCCTGGCCCTCGATGCTTCCCCCCTGAGGGGAGGCATCGTGAGGGACTGT
  GGCAGGCTTCACTGAACGCTGAGCCGGGGAGGTCCAACTCCACGTATGGATCCGGGGAAT
  GAGAATTCAGCCACAGAGGCTGCCGCGATCATAGACCTAGATCCCGACTTCGAACCCCAG
  AGCCGTCCCCGCTCCTGCACCTGGCCCCTTCCCCGACCAGAGATCGCTAACCAGCCGTCC
  GAGCCGCCCGAGGTGGAGCCAGATCTGGGGGAAAAGGTACACACGGAGGGGCGCTCAGAG
  CCGATCCTGTTGCCCTCTCGGCTCCCAGAGCCGGCCGGNGGCCCCCAGCCCGGAATCCTN
  GGGGCTGTAACAGGTCCTCGGAAGGGAGGCTCCCGCCGGAATGCCTGGNGAAATCAGTCA
  TATGCAGAACTCATCAGCCAGGCCATTGAAAGCGCCCCGGAGAAGCGATTGACACTTGCC
  CAGATCTACGAGTGGATGGTCCGTACTGTACCCTACTTCAAGGAACAAGGTGACAGNCAC
  AGCTCANCAGGATGGGAGAACTCGATCCGNCCAACCTGTCCCTGCACACAAGTTCATCAA
  GGTTCACCACGAGGCCAACGGCAAAAGCTCTGGNTNATGCTGAACCCTGAGGNAGGCAGA
  GCGGCAAAGCCCCCCCCGCCCGGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005938 unedited
  CCCCTCGAAAAGNCATTTTTTTCATTTCATTGCATTTGNACACACTTGCCCCACTCCAAT
  CTAGCACATGATACAATCTGGGTAGGCCAGGCGCTGACACAGGAAATGATGGGAACCCAC
  AGCAGGGGTGGGGGAGGGGACGATGCAAAGGGCACTGGGGGAAGGGCTGCTTTGGGGGCT
  GGGGTGGAGAAGGAGCCCCAGATGGCCTCCCTGGCAGGTGCTAGTAAACCTGATCCACAT
  CCCCCCCCATCCCTGCTCATTTCCCAGACCCTCAGCCAAGCTCCCCAGCTCCCCCTTCCC
  TGTTCCTCCCCTCCCCGCTGCCTGCTGCCACACACACTTCCACATAACCCCTGCAGTCAG
  GGAAGGGGAAGAAGGGCAGGGACAGCTGTCAGGGGCCTTGAACAGGGAGGTCTCTGTACC
  TAACTTTACCATCCTAAATCCTTCCCTCCTTCTCTTCTCTCCCCTCCCAGTCCCTTCCCT
  GGCCCCAAGCTAATTCCAGGCCTTCAATGTACTCTCCCACCATAGCACTAACCCATGCTG
  GGTCATAGAAGAGTGTGGCTGCACCCTAACACCACACACTCCCAGGCACAAGTTGAAGGA
  ATGAGGGGACCTGGGTTAGCTTTTTTGTTAGGGAAACTCTAACCTATTTCTGCATAGACC
  TTTCTCTATACCTGGTCCGTAGGGGAGCTCTTCCCTGTGTTTACTATTACTCCCCACCTC
  TCCCCACTTCCCTGTGTCTCCTAATATTTATTGTCTGTAGTGTTTGTATCATGTCACTAT
  CACAAAGCCTTCAGATAGAATAAAAAGCATTCTGCCCACCACTAGCACCCTGCCATGGTT
  AAAAAATCTCTATATTTGAGTTTATATTTCTTTAAAAGTTTATAAAAAGCCTTTCTGTTA
  TCTGCAGCCTTCTAAGGGTGATTTGGCCTGGCGAGCCACTCCAGTGCTCAGACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005938
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005938.1, NP_005929.1
• RefSeq Size: 3171 bp
• RefSeq ORF: 1506 bp
• Locus ID: 4303
• UniProt ID: P98177
• Cytogenetics: Xq13.1
• Domains: FH
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1 also known as AFX alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.