TAB1 (NM_006116) Human Untagged Clone

CAT#: SC116337

TAB1 (untagged)-Human TGF-beta activated kinase 1/MAP3K7 binding protein 1 (TAB1), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAB1
• Synonyms: 3'-Tab1; MAP3K7IP1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116337 sequence for NM_006116 edited (data generated by NextGen Sequencing)
  ATGGCGGCGCAGAGGAGGAGCTTGCTGCAGAGTGAGCAGCAGCCAAGCTGGACAGATGAC
  CTGCCTCTCTGCCACCTCTCTGGGGTTGGCTCAGCCTCCAACCGCAGCTACTCTGCTGAT
  GGCAAGGGCACTGAGAGCCACCCGCCAGAGGACAGCTGGCTCAAGTTCAGGAGTGAGAAC
  AACTGCTTCCTGTATGGGGTCTTCAACGGCTATGATGGCAACCGAGTGACCAACTTCGTG
  GCCCAGCGGCTGTCCGCAGAGCTCCTGCTGGGCCAGCTGAATGCCGAGCACGCCGAGGCC
  GATGTGCGGCGTGTGCTGCTGCAGGCCTTCGATGTGGTGGAGAGGAGCTTCCTGGAGTCC
  ATTGACGACGCCTTGGCTGAGAAGGCAAGCCTCCAGTCGCAATTGCCAGAGGGAGTCCCT
  CAGCACCAGCTGCCTCCTCAGTATCAGAAGATCCTTGAGAGACTCAAGACGTTAGAGAGG
  GAAATTTCGGGAGGGGCCATGGCCGTTGTGGCGGTCCTTCTCAACAACAAGCTCTACGTC
  GCCAATGTCGGTACAAACCGTGCACTTTTATGCAAATCGACAGTGGATGGGTTGCAGGTG
  ACACAGCTGAACGTGGACCACACCACAGAGAACGAGGATGAGCTCTTCCGTCTTTCGCAG
  CTGGGCTTGGATGCTGGAAAGATCAAGCAGGTGGGGATCATCTGTGGGCAGGAGAGCACC
  CGGCGGATCGGGGATTACAAGGTTAAATATGGCTACACGGACATTGACCTTCTCAGCGCT
  GCCAAGTCCAAACCAATCATCGCAGAGCCAGAAATCCATGGGGCACAGCCGCTGGATGGG
  GTGACGGGCTTCTTGGTGCTGATGTCGGAGGGGTTGTACAAGGCCCTAGAGGCAGCCCAT
  GGGCCTGGGCAGGCCAACCAGGAGATTGCTGCGATGATTGACACTGAGTTTGCCAAGCAG
  ACCTCCCTGGACGCAGTGGCCCAGGCCGTCGTGGACCGGGTGAAGCGCATCCACAGCGAC
  ACCTTCGCCAGTGGTGGGGAGCGTGCCAGGTTCTGCCCCCGGCACGAGGACATGACCCTG
  CTAGTGAGGAACTTTGGCTACCCGCTGGGCGAAATGAGCCAGCCCACACCGAGCCCAGCC
  CCAGCTGCAGGAGGACGAGTGTACCCTGTGTCTGTGCCATACTCCAGCGCCCAGAGCACC
  AGCAAGACCAGCGTGACCCTCTCCCTTGTCATGCCCTCCCAGGGCCAGATGGTCAACGGG
  GCTCACAGTGCTTCCACCCTGGACGAAGCCACCCCCACCCTCACCAACCAAAGCCCGACC
  TTAACCCTGCAGTCCACCAACACGCACACGCAGAGCAGCAGCTCCAGCTCTGACGGAGGC
  CTCTTCCGCTCCCGGCCCGCCCACTCGCTCCCGCCTGGCGAGGACGGTCGTGTTGAGCCC
  TATGTGGACTTTGCTGAGTTTTACCGCCTCTGGAGCGTGGACCATGGCGAGCAGAGCGTG
  GTGACAGCACCGTAG
  
  Clone variation with respect to NM_006116.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006116 unedited
  NNGGCTCCTTCCCCCGCCCGTTCCCTCAAAGGGCGGTAGGCGTGTACGGTGGGAAGGTCT
  ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG
  CGGCCGCGAATTCGGCACGAGGGGGGTTCCTCCAAGATGGCGGCGCAGAGGAGGAGCTTG
  CTGCAGAGTGAGCAGCAGCCAAGCTGGACAGATGACCTGCCTCTCTGCCACCTCTCTGGG
  GTTGGCTCAGCCTCCAACCGCAGCTACTCTGCTGATGGCAAGGGCACTGAGAGCCACCCG
  CCAGAGGACAGCTGGCTCAAGTTCAGGAGTGAGAACAACTGCTTCCTGTATGGGGTCTTC
  AACGGCTATGATGGCAACCGAGTGACCAACTTCGTGGCCCAGCGGCTGTCCGCAGAGCTC
  CTGCTGGGCCAGCTGAATGCCGAGCACGCCGAGGCCGATGTGCGGCGTGTGCTGCTGCAG
  GCCTTCGATGTGGTGGAGAGGAGCTTCCTGGAGTCCATTGACGACGCCTTGGCTGAGAAG
  GCAAGCCTCCAGTCGCAATTGCCAGAGGGAGTCCCTCAGCACCAGCTGCCTCCTCAGTAT
  CAGAAGATCCTTGAGAGACTCAAGACGTTAGAGAGGGAAATTTCGGGAGGGGCCATGGCC
  GTTGTGGCGGTCCTTCTCAACAACAAGCTCTACGTCGCCAATGTCGGTACAAACCGTGCA
  CTTTTATGCAAATCGACAGTGGATGGGTTGCAGGTGACACAGCTGAACGTGGACCACACC
  ACAGAGAACGAGGATGAGCTCTTCCGTCTTTCGCAGCTGGGCTTGGATGCTGGAAAGATC
  AAGCAGGTGGGGGATCATCTGTGGGCAGGAGAGCACCCGGCGGATCGGGGATTACAAGGT
  AAATATGGCTACACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006116 unedited
  GCGGTATACCAGTACGCGCCCCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  AATATCATAATGTGTAATGCCACCATTTATGACTTCTTTACTACATTAAAAAATGACCCT
  GTTTCTAGAGACCAAACAGCACCACAGAGGCATTCTTGGAAGAAAATTCCTTTTGTTCCC
  AAGCCTTTGTGACTGACTTTAAATCCTCTCACCTGCTTTAATAAAGATGGCTTCAAAGTG
  GGGGGGGGGGGAGTGAGCGAGGACCCTGGGCTGAGACCTGTTTTTCTTCCATTTCTGCTG
  TGGCTTCCCACAGCTCCCTGGTTCCACACCAAGCCCTGCTCTGCCGCAAGAAAATGGATT
  CCCAGGCCACAAAGCTGTTAGGCCTTTGACTTTGCAAAAACCAAAAACCCCAAAAGCTGG
  GCCACAGCGGCTAAACCCTGGTGGGCCCGTCCGGGGCATGGGGGGCACGGACACTGGCAG
  ATGGGGAGGGCGTCCAAAAAACCAGGGGATCTGGGAGGAGGAACAACAGGGTATACGACT
  AACCACGGCTGACCCCCCGGACCCACGGATCAACCAAAGTTCCGACTACCCCCCGCCCAT
  TACCATCAACACCGCACAGACAAACCCATCGCCCCGCAACAGACCGGGGGACAAACTGCG
  CGAACACCCCGCAGAGAAATGAGGCTGCCAAACGCCCACACAGAGGAACTCCCCCGGGCC
  CGTCCACACACCTCTTCTCCTCCTCTCACCATCCTCACACACACACCCCCCACCCCAGGA
  CACTCCTCCACCACATTATATATAAATTAACCCTCCCTATAACGATAATAATAGCGCGTC
  ACACCCCCCCCAAAATATGAAACACACCATTTAACGCACCGCCTTCAATCATTGACACCC
  CAAATAAAGACATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006116
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006116.2, NP_006107.1
• RefSeq Size: 3240 bp
• RefSeq ORF: 1515 bp
• Locus ID: 10454
• UniProt ID: Q15750
• Cytogenetics: 22q13.1
• Domains: PP2C
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, NOD-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (alpha) encodes the longer isoform (alpha).