AATF (NM_012138) Human Untagged Clone

CAT#: SC115532

AATF (untagged)-Human apoptosis antagonizing transcription factor (AATF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AATF
• Synonyms: BFR2; CHE-1; CHE1; DED
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115532 sequence for NM_012138 edited (data generated by NextGen Sequencing)
  ATGGCGGGGCCGCAGCCCCTGGCGCTGCAACTGGAACAGTTGTTGAACCCGCGACCAAGC
  GAGGCGGACCCTGAAGCGGACCCCGAGGAAGCCACTGCTGCCAGGGTGATTGACAGGTTT
  GATGAAGGGGAAGATGGGGAAGGTGATTTCCTAGTAGTGGGTAGCATTAGAAAACTGGCA
  TCAGCCTCCCTCTTGGACACGGACAAAAGGTATTGCGGCAAAACCACCTCTAGAAAAGCA
  TGGAATGAAGACCATTGGGAGCAGACTCTGCCAGGATCGTCTGATGAGGAAATATCTGAT
  GAGGAAGGGTCTGGAGATGAAGATTCAGAGGGACTGGGTCTGGAGGAATATGATGAGGAC
  GACCTGGGTGCTGCTGAGGAACAGGAGTGTGGTGATCACAGGGAGAGCAAGAAGAGCAGA
  AGCCACTCTGCAAAAACACCGGGCTTCAGTGTCCAGAGTATCAGTGACTTTGAGAAATTT
  ACCAAGGGAATGGATGACCTTGGGAGCAGTGAGGAGGAGGAAGACGAAGAGAGTGGCATG
  GAAGAAGGGGATGACGCGGAAGACTCCCAAGGCGAGAGTGAGGAAGACAGGGCTGGAGAT
  AGAAACAGTGAGGATGATGGTGTGGTGATGACCTTCTCTAGTGTCAAAGTTTCTGAGGAA
  GTGGAGAAAGGAAGAGCCGTGAAGAACCAGATAGCACTGTGGGACCAGCTCTTGGAAGGA
  AGGATCAAACTACAAAAAGCTCTGTTGACCACCAACCAGCTTCCTCAACCAGATGTTTTC
  CCATTGTTCAAGGACAAAGGTGGCCCAGAATTTTCCAGTGCCCTGAAAAATAGTCACAAG
  GCACTTAAAGCATTGTTGAGGTCATTGGTAGGTCTTCAGGAAGAGTTGCTTTTCCAGTAC
  CCAGACACTAGATATCTAGTAGATGGGACAAAGCCCAATGCGGGAAGTGAGGAGATTTCT
  AGTGAAGATGATGAGCTGGTAGAAGAGAAGAAGCAGCAACGAAGAAGGGTCCCTGCAAAG
  AGGAAGCTGGAGATGGAGGACTATCCCAGCTTCATGGCAAAGCGCTTTGCCGACTTTACA
  GTCTACAGGAACCGCACACTTCAGAAATGGCACGATAAGACCAAACTGGCTTCTGGAAAA
  CTGGGGAAGGGTTTTGGTGCCTTTGAACGCTCAATCTTGACTCAGATCGACCATATTCTG
  ATGGACAAAGAGAGATTACTTCGAAGGACACAGACCAAGCGCTCTGTCTATCGAGTTCTT
  GGCAAACCTGAGCCAGCAGCTCAGCCTGTCCCAGAGAGTTTGCCAGGGGAACCGGAGATC
  CTTCCTCAAGCCCCTGCTAATGCTCATCTGAAGGACTTGGATGAAGAAATCTTTGATGAT
  GATGACTTTTACCACCAGCTCCTTCGAGAACTCATAGAACGGAAGACCAGCTCCTTGGAT
  CCCAACGATCAGGTGGCCATGGGAAGGCAGTGGCTTGCAATCCAGAAGTTACGAAGCAAA
  ATCCACAAAAAAGTAGATAGGAAAGCCAGCAAAGGCAGGAAACTTCGGTTTCATGTCCTT
  AGCAAGCTACTGAGTTTCATGGCACCTATTGACCATACTACAATGAATGATGATGCCAGG
  ACAGAACTGTACCGCTCTCTTTTTGGCCAGCTCCACCCTCCCGACGAAGGCCACGGGGAT
  TGA
  
  Clone variation with respect to NM_012138.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012138 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCTCGTGCCGAATTCGGCACG
  AGGCATTTACGTGCGCGAAGCGGAGTGGACCGGGAGCTGGTGACGATGGCGGGGCCGCAG
  CCCCTGGCGCTGCAACTGGAACAGTTGTTGAACCCGCGACCAAGCGAGGCGGACCCTGAA
  GCGGACCCCGAGGAAGCCACTGCTGCCAGGGTGATTGACAGGTTTGATGAAGGGGAAGAT
  GGGGAAGGTGATTTCCTAGTAGTGGGTAGCATTAGAAAACTGGCATCAGCCTCCCTCTTG
  GACACGGACAAAAGGTATTGCGGCAAAACCACCTCTAGAAAAGCATGGAATGAAGACCAT
  TGGGAGCAGACTCTGCCAGGATCGTCTGATGAGGAAATATCTGATGAGGAAGGGTCTGGA
  GATGAAGATTCAGAGGGACTGGGTCTGGAGGAATATGATGAGGACGACCTGNGTGCTGCT
  GAGGAACAGGAGTGTGGTGATCACAGGGAGAGCAAGAAGAGCAGAAGCCACTCTGCAAAA
  ACACCGGGCTTCAGTGTCCAGAGTATCAGTGACTTTGAGAAATTTACCAAGGGAATGGAT
  GACCTTGGGAGCAGTGAGGAGGAGGAAGACGAAGAGAGTGGCATGGAAGAAGGGGATGAC
  GCGGAAGACTCCCAAGCGAGAGTGAGGAAGACAGGGCTGGAGATAGAACAGTGAGATGAT
  GGGTGTGGGNGATGACCTTCTCTATGTCAAAGTTTCTGAGGAAGTGGAGAAAGAAAAGCC
  GTGAAGACCAGNAAGCACTGTGGGACAGCTCTTGGAGGGAAGATCAACTACAAAAGCTCT
  GTTGACCACAACAGCTTCTCAACAGAGTTTTCCATTGTCAGCACAAGGTGCCANAATTTN
  CAGTGCTGAAATAGTCCAGACTAAGCATTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012138 unedited
  GGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGCGGTAACAATGCTCTTTATT
  TGTGGCGTTTAAAGGCGGGGTTGGGCGGGACAGCGCCCTTGCGTGTATTAGGCACAGGTT
  CGGAACGCAGCATCTTTCCAGGGGCTTCCCTACTAGCTCAGCCCCATAAAGTCACTGGTT
  TCCTCCATCTGGACCAGCAGCCGCACCAGCCAAGGCGCCCACTGGGTGTCGGAGGTGGGC
  GATGTCAATCCCCGTGGCCTTCGTCGGGAGGGTGGAGCTGGCCAAAAAGAGAGCGGTACA
  GTTCTGTCCTGGCATCATCATTCATTGTAGTATGGTCAATAGGTGCCATGAAACTCAGTA
  GCTTGCTAAGGACATGAAACCGAAGTTTCCTGCCTTTGCTGGCTTTCCTATCTACTTTTT
  TGTGGATTTTGCTTCGTAACTTCTGGATTGCAAGCCACTGCCTTCCCATGGCCACCTGAT
  CGTTGGGATCCAAGGAGCTGGTCTTCCGTTCTATGAGTTCTCGAAGGAGCTGGTGGTAAA
  AGTCATCATCATCAAAGATTTCTTCATCCAAGTCCTTCAGATGAGCATTAGCAGGGGCTT
  GAGGAAGGATCTCCGGTTCCCCTGGCAAACTCTCTGGGACAGGCTGAGCTGCTGGCTCAG
  GTTTGCCAAGAACTCGATAGACAGAGCGCTTGGTCTGTGTCCTTCGAAGTAATCTCTCTT
  TGTCCATCAGAATATGGTCGATCTGAGTCAAGATTGAGCGTTCAAAGGCACCCAAAACAC
  TTCCCCNNAGTTTCCAGAAGCCAGTNTTGGTCTTTATCGTGCCATTTCTGAAGTGTGCGG
  TTCCTGTAGACTGGTAAGTCCGCAAAGCGCTTTGCCATGAGCTGGGATAGTCCTCCATCT
  CCAGCTTTCTCTTTCAGGGACCCTNNCTCNGTGCTGCGTTCTTCCTTCTACAGCTCATCA
  TCTTCACTAAAATCTCCTCCCTCCCGCATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012138
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012138.2, NP_036270.1
• RefSeq Size: 2081 bp
• RefSeq ORF: 1683 bp
• Locus ID: 26574
• UniProt ID: Q9NY61
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]