TMS1 (PYCARD) (NM_013258) Human Untagged Clone

CAT#: SC115313

PYCARD (untagged)-Human PYD and CARD domain containing (PYCARD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMS1
• Synonyms: ASC; CARD5; TMS; TMS-1; TMS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_013258 edited
  GAATTCGGCACGAGGCATGGGGCGCGCGCGCGACGCCATCCTGGATGCGCTGGAGAACCT
  GACCGCCGAGGAGCTCAAGAAGTTCAAGCTGAAGCTGCTGTCGGTGCCGCTGCGCGAGGG
  CTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCATGGACGCCTTGGACCTCACCGACAA
  GCTGGTCAGCTTTTACCTGGAGACCTACGGCGCCGAGCTCACCGCTAACGTGCTGCGCGA
  CATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGCGGCCACGCACCAGGGCTCTGGAGC
  CGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGCAGCCAAGCCAGGCCTGCACTTTAT
  AGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCACAAACGTTGAGTGGCTGCTGGATGC
  TCTGTACGGGAAGGTCCTGACGGATGAGCAGTACCAGGCAGTGCGGGCCGAGCCCACCAA
  CCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACCAGCCTGGAACTGGACCTGCAAGGA
  CTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTACCTGGTGGAGGACCTGGAGCGGAG
  CTGAGGCTCCTTCCCAGCAACACTCCGGTCAGCCCCTGGCAATCCCACCAAATCATCCTG
  AATCTGATCTTTTTATACACAATATACGAAAAGCCAGCTTGAAAAAAAAAAAAAAAAAAC
  TCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013258 unedited
  TACGACTCTCTATAGGGCGGCCGCGAATTCGGCACGAGGCATGGGGCGCGCGCGCGACGC
  CATCCTGGATGCGCTGGAGAACCTGACCGCCGAGGAGCTCAAGAAGTTCAAGCTGAAGCT
  GCTGTCGGTGCCGCTGCGCGAGGGCTACGGGCGCATCCCGCGGGGCGCGCTGCTGTCCAT
  GGACGCCTTGGACCTCACCGACAAGCTGGTCAGCTTTTACCTGGAGACCTACGGCGCCGA
  GCTCACCGCTAACGTGCTGCGCGACATGGGCCTGCAGGAGATGGCCGGGCAGCTGCAGGC
  GGCCACGCACCAGGGCTCTGGAGCCGCGCCAGCTGGGATCCAGGCCCCTCCTCAGTCGGC
  AGCCAAGCCAGGCCTGCACTTTATAGACCAGCACCGGGCTGCGCTTATCGCGAGGGTCAC
  AAACGTTGAGTGGCTGCTGGATGCTCTGTACGGGAAGGTCCTGACCGATGAGCAGTACCA
  GGCAGTGCGGGCCGAGCCCACCAACCCAAGCAAGATGCGGAAGCTCTTCAGTTTCACACC
  AGCCTGGAACTGCACCTGCAAAGACTTGCTCCTCCAGGCCCTAAGGGAGTCCCAGTCCTA
  CCTGGTGGAGGACCTGGAACGGAGCTGAAGCTTCTTCCCACACACACTTCCGTTAAGCCC
  CTGCCAATCCCACCAAATAATCCTGAATCTGATCTTTTATTCACCTTTTACGAAAAGCCC
  CTTTGAAAAAAAAAAAAAAAACTTCACCTTAATTGCGGCCCCGGGCATATTTTTTCCCTG
  ACAAATCCCCGGGGGATCTCTGGAACCCTCCCAAAGCCTTTCCTGGGCCTGAAAATTGGA
  CCTCCAGGGGCCCCCAACCTTGTCCTAAAAAATAAAATTGCCAATTTGGTCGCCACGGGG
  GCCCCTTTAAAATATCGGGGGAAGGGGGGGGTTCTGACCCACGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013258 unedited
  GTACCGCGGCNCGCATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTCAAGCTGGCTTTTC
  GTATATTGTGTATAAAAAGATCAGATTCAGGATGATTTGGTGGGATTGCCAGGGGCTGAC
  CGGAGTGTTGCTGGGAAGGAGCCTCAGCTCCGCTCCAGGTCCTCCACCAGGTAGGACTGG
  GACTCCCTTAGGGCCTGGAGGAGCAAGTCCTTGCAGGTCCAGTTCCAGGCTGGTGTGAAA
  CTGAAAAGCTTCCGCATCTTGCTTGGGTTGGTGGGCTCGGCCCGCACTGCCTGGTACTGC
  TCATCCGTCAGGACCTTCCCGTACAGAGCATCCACCAGCCACTCAACGTTTGTGACCCTC
  CCGATAAGCGCACCCCGGTGCTGGTCTATAAAGCGCACGCCTGGCTTGGCTGCCGACTGA
  GGAGGGGCCTGGATCCCACCTGGGGCGGCTTCACAGCCCTGGTGCCTGGCCGCCTGCCCC
  TGCTCCGCCCTCTCCTGCAGGCCCCTTGCCCCCCATCGCCCTCCCGGTCACTTTCGCGCT
  CATCGTCTCCCCCGTTCACCCCCTCCCTTTCTTCGCTAGCTCCCCCCTTTTCCTTCACCT
  TCCCCCCTCCCCCATACCTCTTCTCCATCTCTCCGTCCTACCCGCCCTATTCTCCGCCCC
  GATTCCTGATCTCCCTCCTTTCCCCGCTTTCTCGCCACCCTCCCCTTCTTCTTTCTCCCT
  CCCCCCACCCCCCTCCCTGTCCCCACCCTCTTCAACCTCTCCCCCCCTTCTATCCCCCGC
  ACCCCCCCCCTACGCCTATACGCCTCTCTAACACCCCCCCCGCTCTTCTCCCCCCCCCCC
  GTTCTCCCCCCCCCCACCTCCTATTGCCTCGCCTGCCCTCCTGTTTTTTCCCTGCCGCCC
  ACGTCTCTTCACCCCATCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013258
• Insert Size: 730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013258.3, NP_037390.2
• RefSeq Size: 936 bp
• RefSeq ORF: 588 bp
• Locus ID: 29108
• UniProt ID: Q9ULZ3
• Cytogenetics: 16p11.2
• Domains: PAAD_DAPIN
• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway
• Gene Summary: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).