ALDH4A1 (NM_003748) Human Untagged Clone

CAT#: SC109971

ALDH4A1 (untagged)-Human aldehyde dehydrogenase 4 family, member A1 (ALDH4A1), nuclear gene encoding mitochondrial protein, transcript variant P5CDhL

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDH4A1
• Synonyms: ALDH4; P5CD; P5CDh
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003748, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCTGCCGGCGCCCGCGCTCCGCCGCGCCCTGCTGTCCCGCCCCTGGACCGGGGCCGGCCTGCGGT
  GGAAGCACACCTCCTCCCTGAAGGTGGCCAACGAGCCCGTCTTAGCCTTCACGCAGGGCAGCCCTGAGCG
  AGATGCCCTGCAAAAGGCCTTGAAGGACCTGAAGGGCCGGATGGAAGCCATCCCATGCGTGGTGGGGGAT
  GAGGAGGTGTGGACGTCGGACGTGCAGTACCAAGTGTCGCCTTTTAACCATGGACATAAGGTGGCCAAGT
  TCTGTTATGCAGACAAGAGCCTGCTCAACAAAGCCATTGAGGCTGCCCTGGCTGCCCGGAAAGAGTGGGA
  CCTGAAGCCTATTGCAGACCGGGCCCAGATCTTCCTGAAGGCGGCAGACATGCTGAGTGGGCCGCGCAGG
  GCTGAGATCCTCGCCAAGACCATGGTGGGACAGGGTAAGACCGTGATCCAAGCGGAGATTGACGCTGCAG
  CGGAACTCATCGACTTCTTCCGGTTCAATGCCAAGTATGCGGTGGAGCTGGAGGGGCAGCAGCCCATCAG
  CGTGCCCCCGAGCACCAACAGCACGGTGTACCGGGGTCTGGAGGGCTTCGTGGCGGCCATCTCGCCCTTT
  AACTTCACTGCAATCGGCGGCAACCTGGCGGGGGCACCGGCCCTGATGGGCAACGTGGTCCTATGGAAGC
  CCAGTGACACTGCCATGCTGGCCAGCTATGCTGTCTACCGCATCCTTCGGGAGGCTGGCCTGCCCCCCAA
  CATCATCCAGTTTGTGCCAGCTGATGGGCCCCTATTTGGGGACACTGTCACCAGCTCAGAGCACCTCTGT
  GGCATCAACTTCACAGGCAGTGTGCCCACCTTCAAACACCTGTGGAAGCAGGTGGCCCAGAACCTGGACC
  GGTTCCACACCTTCCCACGCCTGGCTGGAGAGTGCGGCGGAAAGAACTTCCACTTCGTGCACCGCTCGGC
  CGACGTGGAGAGCGTGGTGAGCGGGACCCTCCGCTCAGCCTTCGAGTACGGTGGCCAGAAGTGTTCCGCG
  TGCTCGCGTCTCTACGTGCCGCACTCGCTGTGGCCGCAGATCAAAGGGCGGCTGCTGGAGGAGCACAGTC
  GGATCAAAGTGGGCGACCCTGCAGAGGATTTTGGGACCTTCTTCTCTGCAGTGATTGATGCCAAGTCCTT
  TGCCCGTATCAAGAAGTGGCTGGAGCACGCACGCTCCTCACCCAGCCTCACCATCCTGGCCGGGGGCAAG
  TGTGATGACTCCGTGGGCTACTTTGTGGAGCCCTGCATCGTGGAGAGCAAGGACCCTCAGGAGCCCATCA
  TGAAGGAGGAGATCTTCGGGCCTGTACTGTCTGTGTACGTCTACCCGGATGACAAGTACAAGGAGACGCT
  GCAGCTGGTTGACAGCACCACCAGCTATGGCCTCACGGGGGCAGTGTTCTCCCAGGATAAGGACGTCGTG
  CAGGAGGCCACAAAGGTGCTGAGGAATGCTGCCGGCAACTTCTACATCAACGACAAGTCCACTGGCTCGA
  TAGTGGGCCAGCAGCCCTTTGGGGGGGCCCGAGCCTCTGGAACCAATGACAAGCCAGGGGGCCCACACTA
  CATCCTGCGCTGGACGTCGCCGCAGGTCATCAAGGAGACACATAAGCCCCTGGGGGACTGGAGCTACGCG
  TACATGCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003748 unedited
  NTTTACACCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGCCGAGATGCTGCTGCCGGCGCCCGCGCTCCGCCGCGCCCTGCTGT
  CCCGCCCCTGGACCGGGGCCGGCCTGCGGTGGAAGCACACCTCCTCCCTGAAGGTGGCCA
  ACGAGCCCGTCTTAGCCTTCACGCAGGGCAGCCCTGAGCGAGATGCCCTGCAAAAGGCCT
  TGAAGGACCTGAAGGGCCGGATGGAAGCCATCCCATGCGTGGTGGGGGATGAGGAGGTGT
  GGACGTCGGACGTGCAGTACCAAGTGTCGCCTTTTAACCATGGACATAAGGTGGCCAAGT
  TCTGTTATGCAGACAAGAGCCTGCTCAACAAAGCCATTGAGGCTGCCCTGGCTGCCCGGA
  AAGAGTGGGACCTGAAGCCTATTGCAGACCGGGCCCAGATCTTCCTGAAGGCGGCAGACA
  TGCTGAGTGGGCCGCGCAGGGCTGAGATCCTCGCCAAGACCCTGGTGGGACAGGGTAAGA
  CCGCGATCCAAGCGGAGATTGACGCTGCAGCGGAACTCATTGACTTCTTTCGGCTCAATG
  CCAGTATGCCGGGGAGCCTGAAGGGCCCAATCCCCTCCACGCGCCCCCGACACCACCAGA
  ACGGGGACCCCGGGGGTTGAAGCCCCCCTCCCCCTTCTCTCTCCCTCACCTTCCCCCCTC
  TCCGGTCCTCCTCTCCGCGCCCCCTTCCCCCTCCTCCCCTCCCCCACCACCCCCCTCCCC
  TACCTTCTTCCTTCCTCTCTTTCCTCATTACTCCTCCTCTCTCTCTCCTACCCCCCTACT
  ACTTCACATCACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003748 unedited
  GGATTCGTGTANGTTGCCGCACTCGANGTCGAGTTATAACCTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTAAAA
  AACAGGCCTTTTATTGGGGGGGATTTGAACAAATCCCATTGCCCCCTCACAGGGGAAAAC
  CCGTAAAACTAAAAATACTCCAGCCTGGGCCCCCTTTTTTTCACCTGCCCCGGCCCCTTT
  TGGGAAAAGACCCATTTCCCGGAAGGGCCCAAAACCCCCACCCAGCCCAGTTGGGACCAG
  CCCCCCCCCCCTTCCATGGGCCAGTGGCCCTTGGGGAATGGCCCCCCCCCCTCCGGGGGG
  GGGCCCCTGCCGGCCTCCTGGAAGAAAACCCAACACTTTTACAGGCCCCTAAAAACCCTC
  CCTGAAAATCATGCCTTACAAATTTTTGACCCCTCAAAATCCAAGCAGGAGGCATTTGGG
  GGAAGAATCGGGGGCACAAATTGGCTGCGTCAAAAAAAAAATGGGCCACTGAGGGACTGG
  GCCCACAAAACCACTCCCAGGCAGAAGAAGATTTTAAAGGGAAAGTCCTTGGGCCATGCT
  GACAAACCCAAAGGCCGGCCTTTGGGGGCTGGGAAAAAGGAAGGCCCATTTTTGCCTTGG
  CCTGGGTCACCTTGGGACAAGGGGGGGGTGACCTCCCTGGGGGGAAAATCCCAAACCCCC
  GGAACCGGGCTCTTAAAGAGGGGAGGGCCCTTCCACGGTGGGGCCCCGGGAGATCCAAAG
  GGGGAGGGTTGTCTCTATGAACAAAGACCCCCTCCAAGGGTTTTCCCAAATATACGGGCC
  CTAAACCGGGAGGGCCCCTCCCTCCCCCCCCCCCTTGGGAGGGGGGGCCTCCAAGGGCCC
  CCACAAACCCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003748
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003748.2, NP_003739.2
• RefSeq Size: 3160 bp
• RefSeq ORF: 1692 bp
• Locus ID: 8659
• UniProt ID: P30038
• Cytogenetics: 1p36.13
• Domains: aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (P5CDhL) represents the longest transcript. Both variants P5CDhL and P5CDhS encode the same protein (isoform a).