Stk36 (NM_001173986) Rat Tagged ORF Clone

CAT#: RR217587

Stk36 (myc-DDK-tagged) - Rat serine/threonine kinase 36 (Stk36)

Product Images

Circular map for RR217587

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stk36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217587 representing NM_001173986
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAGTACCACGTTTTGGAGATGATTGGAGAAGGCTCTTTTGGGAGAGTGTATAAGGGCCGGAAAA
  AATACAGTGCTCAGGTGGTGGCCTTGAAGTTCATCCCCAAACTGGGGCGCTCAGAGAAAGAGCTGAGGAA
  TCTGCAACGAGAGATTGAAATCATGCGGGGTCTGTGGCATCCCAACATTGTGCATATGCTTGACAGCTTC
  GAGACTGACAAGGAGGTGGTGGTGGTGACAGACTATGCTGAGGGAGAGCTCTTTCAGATTCTGGAAGATG
  ATGGAAAACTTCCTGAAGAGCAGGTTCAGGCCATCGCCGCCCAGTTGGTGTCAGCCCTGTACTATCTGCA
  TTCCCACCGCATCCTACACCGAGACATGAAACCTCAGAACATTCTTCTCGCCAAGGGCGGTGGCATCAAA
  CTCTGTGACTTTGGATTTGCCCGAGCTATGAGCACCAACACCATGGTGCTGACCTCAATCAAAGGCACAC
  CGCTCTATATGTCTCCAGAACTGGTGGAGGAGCGACCATATGACCACACGGCAGACCTCTGGTCTGTAGG
  CTGCATCCTGTATGAGCTGGCTGTAGGCACACCTCCCTTCTACACCACCAGCATCTTTCAACTGGTTAGC
  CTCATTCTCAAGGACCCTGTGCGCTGGCCCTCCACCATTACTTCCTGCTTCAAGAACTTCTTGCAGGGGC
  TGCTCACTAAGGACCCCCGGCAGCGTCTGTCCTGGCCAGACCTCTTACATCACCCCTTTATTGCCGGCCG
  TGTCACCATAATAACCGAACCAGCAGGCTCCAACTTGGGCACCCCATTTACTAGTCGCCTACCCCCAGAA
  CTTCAGGTCCTAAAAGATGAACAGGCCCATCGACTGGCACCCAAGGGTAACCAGTCCCGCATCCTGCGCC
  AGGCCTGTAAACGCATGGCTGAAGAAGCCAAGCAGAAGGAAGACCAAAATACAGGATCCACCCTTGATCA
  AGAAGCTAGGCTCAGCAAGGTGACACCCAGCACAGCCCCTGTGCCTGGTCTAAAGGCCACTCCTCAGGAG
  TCAAGCCTCTTGGCTGGCTTACTGGCTTCAGAAATGAAGAACAACTGGGAAGACTGGGGGGCTGGAGAAG
  CACCCCCTACACCTCGGGAAAACCACATCACCTTGGAGTGTGAACAAGCCTTCCCTGAGCTGAGGCCAGA
  GACGATGGGTCGGCGGAGCACCGATGCAGTGGATCCTGAAAACGAGGAGCCAGACAGTGATGACGAGTGG
  CAACGCTTACTCGAGACTATGGAGCCAGTGCCTGTGCAGCTGAAGTCCCCCCTCACCCTGCTGTGTAATC
  CTGACTTCTGCCAGCATATCCAGAGTCAGCTGCGCGGGACTGGCGAGCAGATCCTGAAAGGCGTCCTGGA
  TGGTGCTTCCCACCTCCTTCCTGCACTCCGCATCCTGAGCAGTCTCCTGTCCAGCTGCAGTGACTCTGTG
  CTCTTGTATTCCTTCTGCCAGGAGGTGGGACTGCCCGAGCTGCCTCTCAGTCTTCTTAGGTACAGCCAGG
  AGAGCAGCAGTATCCAGCAGCAACCTTGGTACGGGGCCTTCTTACGGGAACTGGTGGCTGTGGTTCAGGC
  CTACTTTTCATGCACCTTCAATCTGGAGAGGAGCCAGACAGGTGACAGCCTACAGGTGTTTGAGGAGGCT
  GCCAGCCTCTTTTTGGACTTGTTGGGGAAGCTGTTGGCCCAACCAGATGATTCCGAGCCGACATTTCGAA
  GGGATAGCCTCATGTGCTTTGCTGTCTTGTGTGAGGCTGTGGATGGGAACAGCCGGGCCATCTCCAAAGC
  CTTTTATGCCAGCTTGTTGACCACACAGCGTGCTGTGTTGGACGGGCTCCTTCACGGCCTAACGGTTCCA
  CAACTCCCTTTCCACACACCACCAGGAGCCCCACAAGTGAGCCAGCCACTGCGGGAGCAGAGTGAAGATG
  TACCCGGAGCCATCTCTTCTGCCCTGGCAGCCATGTGCACTGCCCCTGTGGGGTTGCCCAGCTGTTGGGA
  CGCCAAGGAGCAGGTCTCTTGGCATTTGGCCAACCAGCTAACCGAGGACAGCAGCCAACTGAGGCCATCC
  CTCATCTCTGGTCTGCGGCACCATATCCTATGCCTCCACCTTCTCAAGGTTCTCTACTCCTGCTGCTATA
  TTAGTGAGCGCCTGTGCCATATTCTGGGACAAGAGCCTCTGGCCTTGGAGTCGCTGCTCATGTTAGTCCA
  GGGCAAGGTGAAAGTAGCAGACTGGGAAGAGTCCACTGAGGTGGCACTTTACCTCCTGTCCCTTCTTGTC
  TTTCGGCTCCAAGATCTACCTTCAGGGATGGAGAAGCTGGGCAGTGAGGTCGCTACTCTCTTCACCCACT
  CACACATCGTCTCTCTTGTGAATGCAGCAGCCTGTCTCTTAGGACAGCTTGGTCAGCAAGGGGTGACCTT
  TGACCTCCAGCCCCGGGAATGGATAGCTGCAGCTGCACATGCCTTGTCTGCCCCTGCAGAGGTCCGGCTG
  ACTCCACCGTGTAGTTGTGGCTTCTATGATGGCCTCCTCGTTCTTCTGCTTCAGCTCCTTACACAGGGGA
  AGCCTGGCCTGATCAGGGACGTGGCAGGTTCAGAGGTGTGGACCATTCTGTGGCACCGCTTTTCCATGGC
  TCTGAGACTATCTGAGGAGGTGTCTGCCCAGGAAGATGACCTGTCACTATCAAGTCCACCCAGCCTAGAG
  CCAGACTGGACACTGATTTCACCCCAAGGCATGGCAGCCTTGCTGAGCCTGGCAATGGCCATCTTCACCC
  AGGAGCCCCAGTTATGCCTGAGCCACCTGTCCCAGCATGGCAGTGTCCTCATGCTGACCCTGAAGCACCT
  GCTTTCACCCAGCTTCTTGCACCGCCTGAGCCAGGCGCCTCAGGGGTCTGAGTTTCTCCCCGTTGTGGTG
  CTCTCTGTGTGCAAGCTGCTCTGCTTCCCCTTCGCCCTGGATGTGGATGCTGACCTCCTCGGAGGCATCT
  TGGCTGACCTCAGGGCCTCAGAAGTTGCAGTCTACCTGCTGCAGGTCTGCTGCCACCACCTTCCATTGTT
  CCAAGCAGAGCTGCCCATTGGCCTCCTTACACGCCTGGCCCTCATGGATCCCACCTCTCTCAAGCAGTTT
  GTGAACACAGTAGCCACCTCTTCTAGAGCCATCATCTCATTCCTCTCTGTTGTTCTCCTGTGTGACCAAC
  CCCTCATGGTCTCTGACCTCCTGTCCCTGCTGACACACACAACCCGGATTCTATCCCCCAGCCACTTGTC
  CTTTATCCAGGAGCTCCTGTCTGGCTCTGATGAATCCTATCGGCCACTGCGCAGCCTCCTAGGCCACTCA
  GAGAATGCTGTGCGGGTCCGTACTTACAGGCTCCTGGGACATTTATTACAACACAGCATGGCCCTACGTG
  GGGCACTACAGAGCCAGTCAGGACTGCTCAACCTTCTGCTGCTAGGGCTTGGAGACAAGGACCCTGCCGT
  GCGACGCAGTGCCAGCTTTGCTGTGGGCAATGCAGCCTACCAGGCTGGTCCCTTGGGACCTGCCTTGGCA
  GCTGCAGTGCCCAGTATGACCCAGCTGCTGGGAGATGCTCAGGATGCTATCCGGCGCAATGCTGCATCAG
  CTCTAGGCAATCTGGGACCTGAAGGATTGGGCAAGGAGCTATTAAAGTGCCAAGTACCCCAGCGGCTCCT
  AGAAATGGCGTGTGGAGACCCTCAACCAACTGTCAAAGAGGCTGCCCTTATTGCCCTCCGGAGCCTCCGA
  CAGGAGCCCTGTATTCATCAGGTGCTGGTGTCGCTGGGTGCCAGTGAGAAATTAACCTTGCTGTCTTTGG
  GGCATCAGTTACTGCCAAGCAGCAATAGCAGGCCTACCTCTGCCAGACACTGCAGGAAGCTTATCCACCT
  CCTGAGGCCGACCCACAGCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217587 representing NM_001173986
  Red=Cloning site Green=Tags(s)
  
  MEKYHVLEMIGEGSFGRVYKGRKKYSAQVVALKFIPKLGRSEKELRNLQREIEIMRGLWHPNIVHMLDSF
  ETDKEVVVVTDYAEGELFQILEDDGKLPEEQVQAIAAQLVSALYYLHSHRILHRDMKPQNILLAKGGGIK
  LCDFGFARAMSTNTMVLTSIKGTPLYMSPELVEERPYDHTADLWSVGCILYELAVGTPPFYTTSIFQLVS
  LILKDPVRWPSTITSCFKNFLQGLLTKDPRQRLSWPDLLHHPFIAGRVTIITEPAGSNLGTPFTSRLPPE
  LQVLKDEQAHRLAPKGNQSRILRQACKRMAEEAKQKEDQNTGSTLDQEARLSKVTPSTAPVPGLKATPQE
  SSLLAGLLASEMKNNWEDWGAGEAPPTPRENHITLECEQAFPELRPETMGRRSTDAVDPENEEPDSDDEW
  QRLLETMEPVPVQLKSPLTLLCNPDFCQHIQSQLRGTGEQILKGVLDGASHLLPALRILSSLLSSCSDSV
  LLYSFCQEVGLPELPLSLLRYSQESSSIQQQPWYGAFLRELVAVVQAYFSCTFNLERSQTGDSLQVFEEA
  ASLFLDLLGKLLAQPDDSEPTFRRDSLMCFAVLCEAVDGNSRAISKAFYASLLTTQRAVLDGLLHGLTVP
  QLPFHTPPGAPQVSQPLREQSEDVPGAISSALAAMCTAPVGLPSCWDAKEQVSWHLANQLTEDSSQLRPS
  LISGLRHHILCLHLLKVLYSCCYISERLCHILGQEPLALESLLMLVQGKVKVADWEESTEVALYLLSLLV
  FRLQDLPSGMEKLGSEVATLFTHSHIVSLVNAAACLLGQLGQQGVTFDLQPREWIAAAAHALSAPAEVRL
  TPPCSCGFYDGLLVLLLQLLTQGKPGLIRDVAGSEVWTILWHRFSMALRLSEEVSAQEDDLSLSSPPSLE
  PDWTLISPQGMAALLSLAMAIFTQEPQLCLSHLSQHGSVLMLTLKHLLSPSFLHRLSQAPQGSEFLPVVV
  LSVCKLLCFPFALDVDADLLGGILADLRASEVAVYLLQVCCHHLPLFQAELPIGLLTRLALMDPTSLKQF
  VNTVATSSRAIISFLSVVLLCDQPLMVSDLLSLLTHTTRILSPSHLSFIQELLSGSDESYRPLRSLLGHS
  ENAVRVRTYRLLGHLLQHSMALRGALQSQSGLLNLLLLGLGDKDPAVRRSASFAVGNAAYQAGPLGPALA
  AAVPSMTQLLGDAQDAIRRNAASALGNLGPEGLGKELLKCQVPQRLLEMACGDPQPTVKEAALIALRSLR
  QEPCIHQVLVSLGASEKLTLLSLGHQLLPSSNSRPTSARHCRKLIHLLRPTHST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173986
• ORF Size: 3942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001173986.1, NP_001167457.1
• RefSeq Size: 5031 bp
• RefSeq ORF: 3945 bp
• Locus ID: 301516
• Cytogenetics: 9q33
• MW: 144.4 kDa