Nlrp4 (NM_001172164) Rat Tagged ORF Clone

CAT#: RR217386

Nlrp4 (myc-DDK-tagged) - Rat NLR family, pyrin domain containing 4 (Nlrp4)

Product Images

Circular map for RR217386

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nlrp4
• Synonyms: Nalp4; Nlrp4e; RGD1563529; RGD1564841
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217386 representing NM_001172164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCATTCTTTTCTGATTTTGGCCTTATGTGGTACTTGGAAGAGTTAAACAAGAAAGAATTCGTGA
  AGTTTAAGGAATTCCTCAAACAGGAGATTTTGCAGTTGAGGCTGAAACAGATTTCTTGGACCAAAGTGAA
  GAAGGCATCTCGGGAAGACCTTGCCAACTTACTGTTGAAACATTATGAGGAGAAGCAAGCCTGGGACATG
  ACCTTCAAAATCTTCCAGAAGATGAACAGGAAGGATCTCATTGAGAGGGCAGGAAGAGAGATTGCTGGTC
  ACTCAAAGTTATATCAAGCTCATTTGAAGAAGAAGCTGACCCATGATTATGCCAGAAAGTTTAACATCAA
  AGCTCAAGATCTCTTTAAGCAGAAATTTACCCAGGATGACTGTGATCGTTTTGAGAACCTTCTTGTATCA
  AAGGCAACTGGAAAGAAGCCACACATGGTGTTCCTACAAGGAGTGGCGGGAATTGGCAAGTCACTGATGT
  TGACAAAGTTAATGCTGGCCTGGTCAGAAGGCATCGTGTTTCAGAACAAATTCTCCTACATCTTCTACTT
  CTGCTGTCAAGATGTGAAGCAGTTAAAGAGAGCAAGTCTGGCAGAACTGATCTCCAGAGAGTGGCCCAAT
  GCCTCGGCTCCCACAGCGGAGATCCTCTCCCAGCCGGAGAAACTCTTGTTTATCATCGACAGCTTGGAAG
  TGATGGAATGCAATATGTCCGAACGGGAATCGGAGCTGTGTGATAACTGCACGGAGAAGCAGCCCGTGAG
  TTTACTGCTGAGCAGTTTGCTCAGGAGGAAGATGCTCCCCGAATCCTCTTTCCTCATCTCCGCTACCCCA
  GAGACTTTTGAGAAAATGGAGGACAGGATTGAGTGCACCAATGTGAAAATAATAACAGGATTCAATGAGA
  ACAATATTAAGATGTATTTCCGCAGCTTGTTCCAAGATAAGAACAGAACCCTGGAGGCCTTCAGTTTAGT
  GAGAGAAAATGAGCAGCTGTTCAATGTATGTCAGGTCCCTGTGCTCTGCTGGATGGTGGCTACCTGTATT
  AAAAAGGAGATAGAGAAGGGAAGAGACCCAGTCTTCATCTGCCGACGTACCACCTCTCTGTACACCACTC
  ACATCTTCAATTTGTTCACTCCACAAAATGCCCAGTATCCAAGTAAGAAAAGCCAAGACCAGCTGCAGGG
  CTTGTGTTCTCTAGCTGCCGAGGGCATGTGGACCGACACATTTGTGTTTAGTGAGGAGGCGCTCAGGAGA
  AATGGAATCCTGGATTCTGACATCCCCACATTGCTGGACAGACGGATCCTTGAAAGGAGCAAGGAATCTG
  AAAGTTGTTACATTTTCCTCCACCCATCTCTCCAGGAGGTCTGTGCTGCCGTCTTTTATCTGCTAAAGAG
  CCACTTGGACCACCCTAGCCAGGACGTAAAGAGTGTAGAGGCACTCTTGTTTACATTTCTGAAGAAAGCC
  AAAGTACAGTGGATCTTTTTAGGCTGTTTCCTCTTTGGCCTTTTACATGAATCGGAACAAGAAAAGCTAG
  AGATGTTTTTCGGCCACCAGTTGTCCCAGGAAATAAAGCATCAGCTGTATCAGTGCCTGGAAACCATAAG
  TGTCAATGAAGAGCTTCAAGAACAGATAGATGGCATGAAGTTGTTTTACTGTCTGTTTGAGATGGAGGAT
  GAAGCCTTCTTAATGCAAGCAATGAACTGTATGGAGCAGATTAACTTTGTGGCTAAGGATTATTCTGATG
  TTATTGTTGCTGCCTACTGCTTAAAACACTGTTCTACCCTGAAGAAACTATCCTTTTCTACCCAAAATAT
  CCTGAGTGAAGAACAAGAACACAGCTATACGGAAAAGCTACTCATCTGTTGGCATCATATGTGCTCTGTG
  CTTATAAGCAGTAAGGACATCCACGTACTCCAAGTAAAAGACACTAATCTCAATGAGACAGCCTTTTGGG
  TCTTGTATAATCATCTGAAGTACCCCAGCTGCACCCTTAAAGTACTCGTGGTCAATAATGTGACCTTCCT
  GTGTGATAACCACCTATTCTTTGAGTTGATTCAGAACCAACGTTTGCAGCACCTGAACCTCAGCCTCACG
  TTCCTCTCCCACAGTGATGTGAAACTGTTGTGTGATGTCTTGAACCAGGCCGAGTGCAACATAGAAAAGC
  TGATGATAGCAGCCTGTAACCTTTCCCCTGATGACTGCAAGGTCTTTGCCTCTGTCCTGATCAGCAGCAA
  GATGTTGAAGCATCTTAATTTGTCATCCAACAACTTGGACAAAGGGATCTCCTCACTGTGCAAGGCTTTG
  TGCCACCCAGACTGCATTCTGAAGCACCTGGTGTTAGCCAACTGTTCCCTCAGTGAGCAATGTTGGGACT
  ATCTCTCAGAAGTTGTTAGGCGGAACAAAACCCTGAGCCACCTAGACATCAGCTCCAATGACCTGAAGGA
  TGAGGGACTGAAAGTTCTCTGTGGAGCTCTGACTCTCCCAGACAGTGGCCTGATATCACTAAGTGTGAGA
  CATTGTCTCATCACCACTAGTGGTTGCCAGGACCTGGCTGAAGTCTTGAGGCACAACCAGAACCTGAGGA
  GCCTACAGGTTTCAAACAATAAGATCGAAGACGCTGGTGTGAAGCTGCTGTGTGATGCTATAAAGCAACC
  CAACTGCCACTTAGAGAATATTGGGTTGGAGGCCTGCGAGCTAACTGGTGCCTGTTGTAAGGATCTTGCT
  TCTGCTTTTGTCCACTGTAAAACCCTGTGGGGTATCAACCTGCTAGAGAACGCCTTGGACCACAGTGGGT
  TGGTCGTGCTCTTTGAGGCTCTGAAACAGCAAAAGTGTACCCTGCATGTTCTTGGACTTCGAATTACTGA
  CTTTGATAAGGAAACCCAGGAGTTCCTGATTGCAGAGGAAGAGAAAAACCCATATTTGAGCATCCTAAGC
  AATGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217386 representing NM_001172164
  Red=Cloning site Green=Tags(s)
  
  MASFFSDFGLMWYLEELNKKEFVKFKEFLKQEILQLRLKQISWTKVKKASREDLANLLLKHYEEKQAWDM
  TFKIFQKMNRKDLIERAGREIAGHSKLYQAHLKKKLTHDYARKFNIKAQDLFKQKFTQDDCDRFENLLVS
  KATGKKPHMVFLQGVAGIGKSLMLTKLMLAWSEGIVFQNKFSYIFYFCCQDVKQLKRASLAELISREWPN
  ASAPTAEILSQPEKLLFIIDSLEVMECNMSERESELCDNCTEKQPVSLLLSSLLRRKMLPESSFLISATP
  ETFEKMEDRIECTNVKIITGFNENNIKMYFRSLFQDKNRTLEAFSLVRENEQLFNVCQVPVLCWMVATCI
  KKEIEKGRDPVFICRRTTSLYTTHIFNLFTPQNAQYPSKKSQDQLQGLCSLAAEGMWTDTFVFSEEALRR
  NGILDSDIPTLLDRRILERSKESESCYIFLHPSLQEVCAAVFYLLKSHLDHPSQDVKSVEALLFTFLKKA
  KVQWIFLGCFLFGLLHESEQEKLEMFFGHQLSQEIKHQLYQCLETISVNEELQEQIDGMKLFYCLFEMED
  EAFLMQAMNCMEQINFVAKDYSDVIVAAYCLKHCSTLKKLSFSTQNILSEEQEHSYTEKLLICWHHMCSV
  LISSKDIHVLQVKDTNLNETAFWVLYNHLKYPSCTLKVLVVNNVTFLCDNHLFFELIQNQRLQHLNLSLT
  FLSHSDVKLLCDVLNQAECNIEKLMIAACNLSPDDCKVFASVLISSKMLKHLNLSSNNLDKGISSLCKAL
  CHPDCILKHLVLANCSLSEQCWDYLSEVVRRNKTLSHLDISSNDLKDEGLKVLCGALTLPDSGLISLSVR
  HCLITTSGCQDLAEVLRHNQNLRSLQVSNNKIEDAGVKLLCDAIKQPNCHLENIGLEACELTGACCKDLA
  SAFVHCKTLWGINLLENALDHSGLVVLFEALKQQKCTLHVLGLRITDFDKETQEFLIAEEEKNPYLSILS
  NV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172164
• ORF Size: 2946 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172164.2, NP_001165635.2
• RefSeq Size: 3387 bp
• RefSeq ORF: 2949 bp
• Locus ID: 499069
• Cytogenetics: 1q12
• MW: 113 kDa