Clock (NM_001289832) Rat Tagged ORF Clone

CAT#: RR217246

Clock (myc-DDK-tagged) - Rat clock circadian regulator (Clock), transcript variant 2

Product Images

Circular map for RR217246

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clock
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217246 representing NM_001289832
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCAATTGTTGACAGAGATGACAGTAGTATTTTTGATG
  GATTGGTGGAAGAAGATGACAAGGACAAAGCAAAAAGAGTATCTAGAAACAAATCAGAAAAGAAACGTAG
  AGATCAGTTCAATGTACTCATTAAGGAACTGGGGTCTATGCTTCCTGGTAATGCTAGAAAGATGGACAAG
  TCTACTGTTCTACAGAAGAGCATTGATTTTTTACGCAAACATAAAGAGATCACTGCACAGTCAGATGCTA
  GTGAAATTCGACAGGACTGGAAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTAGAGGC
  TCTTGATGGTTTTTTTTTAGCGATCATGACAGATGGAAGTATAATATATGTATCTGAGACGGTAACTTCG
  TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAGGGAGAACATT
  CAGAGGTTTATAAGATACTATCTACTCACCTGCTGGAAAGTGACTCCTTAACCCCCGAGGACTTAAAATC
  AAAAAATCAGTTAGAATTCTGTTGTCACATGCTTCGAGGAACAATAGACCCAAAGGAGCCATCCACCTAT
  GAATATGTGAGATTTATAGGAAATTTTAAATCTTTAAACAGTGTATCAACTTCAACACATAATGGTTTCG
  AAGGAACTATACAGCGCACACACAGGCCTTCTTATGAAGATAGAGTTTGTTTCGTAGCTACTGTCAGATT
  AGCTACCCCTCAGTTCATCAAGGAAATGTGTACTGTTGAAGAACCGAATGAAGAGTTCACATCTAGACAC
  AGTTTAGAATGGAAGTTTCTATTTCTAGATCACAGGGCGCCGCCAATCATAGGCTATCTGCCGTTTGAAG
  TCCTGGGAACATCAGGCTATGATTACTATCATGTGGACGACCTAGAAAGTCTGGCAAAATGTCATGAGCA
  CTTAATGCAATATGGAAAAGGCAAATCATGTTACTATAGGTTCTTGACCAAAGGACAGCAGTGGATTTGG
  CTTCAGACTCATTACTATATCACTTACCATCAGTGGAACTCAAGGCCAGAGTTCATCGTTTGTACTCACA
  CTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGGCGAGAACTTGGCGTTGAGGAGTCTCTTCCCGA
  GACAGCTGCTGACAAAAGCCAAGATTCTGGGTCTGACAATCGTATAAACACAGTGAGTCTCAAGGAAGCA
  CTGGAAAGGTTCGATCACAGCCCAACTCCTTCTGCCTCCTCCAGAAGCTCAAGAAAGTCCTCGCACACAG
  CAGTCTCAGACCCTTCCTCAACGCCGACAAAGATCCCCACTGATACTAGCACTCCTCCCAGACCGCACCT
  GCCAGCTCATGAGAAGATGACACAGCGGAGGTCATCCTTCAGTAGTCAGTTTCAGCTTTCAGCTCAGTTA
  GGAGCCATGCAGCATCTCAAAGACCAGCTAGAGCAGCGGACACGCATGATAGAAGCAAATATTCATCGGC
  AGCAAGAAGAACTAAGGAAAATTCAAGAACAACTTCAAATGGTCCATGGTCAAGGGCTACAGATGTTTTT
  GCAGCAATCAAACCCTGGACTGAATCTTGGTTCTGTTCAACTTTCCTCTGGAAATTCCAATATCCAGCAA
  CTCACACCTATAAATATGCAAGGCCAAGTTGTCCCTGTTAACCAGATTCAGAGTGGGGTGAATGCTGGCC
  ATGTCAGCACAGGCCAGCACATGATCCAGCAGCAGACTTTACAAAGTACATCAACTCAGAGTCAACAGAG
  TGTAATGAGTGGACACAGTCAGCCAACGTCTCTTCCAAACCAGACGCCGAGCACTCTGACAGCCCCACTG
  TACAATACGATGGTGATCTCTCAGCCTGCGGCTGGGAGCATGGTCCCGATTCCATCCAGTATGCCGCAGA
  ATAGCACCCAGAGTGCTACAGTCACCACGTTCACTCAGGACAGACAGATAAGGTTTTCTCAAGGTCAGCA
  ACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGTGGGGCCGTCATGGTGCCAAGTACCATGCTTATG
  GGTCAGGTAGTGACTGCCTATCCTACTTTTGCCACACAACAGCAGCAGGCACAGGCCTTGTCAGTAACAC
  AGCAACAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCCGCAGCAGGCGCAGCAGCC
  CCAGTCACAGCAGAGTTCCCAGGACCAGCCGCATCCTTCAGTTCAGCAGCCAGCTCAGCTGACCCAGCCA
  CCACAACAGTTCTTACAGACATCTCGGTTGCTCCATGGGAATCCTTCGACACAGCTCATCCTCTCCGCTG
  CCTTTCCACTACAACAGAGCACTTTCCCTCCTTCGCACCACCAGCAACACCAGCAACAGCAGCTCCATCG
  GCACAGGACTGACAGCCTGACTGACCCTTCCAAGGTCCAGCCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217246 representing NM_001289832
  Red=Cloning site Green=Tags(s)
  
  MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDK
  STVLQKSIDFLRKHKEITAQSDASEIRQDWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSETVTS
  LLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEDLKSKNQLEFCCHMLRGTIDPKEPSTY
  EYVRFIGNFKSLNSVSTSTHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRH
  SLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLESLAKCHEHLMQYGKGKSCYYRFLTKGQQWIW
  LQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGVEESLPETAADKSQDSGSDNRINTVSLKEA
  LERFDHSPTPSASSRSSRKSSHTAVSDPSSTPTKIPTDTSTPPRPHLPAHEKMTQRRSSFSSQFQLSAQL
  GAMQHLKDQLEQRTRMIEANIHRQQEELRKIQEQLQMVHGQGLQMFLQQSNPGLNLGSVQLSSGNSNIQQ
  LTPINMQGQVVPVNQIQSGVNAGHVSTGQHMIQQQTLQSTSTQSQQSVMSGHSQPTSLPNQTPSTLTAPL
  YNTMVISQPAAGSMVPIPSSMPQNSTQSATVTTFTQDRQIRFSQGQQLVTKLVTAPVACGAVMVPSTMLM
  GQVVTAYPTFATQQQQAQALSVTQQQQQQQQQQQQQQQQQPQQAQQPQSQQSSQDQPHPSVQQPAQLTQP
  PQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPPSHHQQHQQQQLHRHRTDSLTDPSKVQPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289832
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289832.1, NP_001276761.1
• RefSeq Size: 9201 bp
• RefSeq ORF: 2499 bp
• Locus ID: 60447
• UniProt ID: Q9WVS9
• Cytogenetics: 14p11
• MW: 94 kDa
• Gene Summary: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with Arntl (Bmal1) that binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Polymorphisms in this gene may be associated with behavioral changes, obesity, and metabolic syndrome. Two transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]